LOCUS       sequence-375234-       11941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express GFP-DHX37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Dong MB, Wang G, Chow RD, Ye L, Zhu L, Dai X, Park JJ, Kim HR, 
            Errami Y, Guzman CD, Zhou X, Chen KY, Renauer PA, Du Y, Shen J, Lam 
            SZ, Zhou JJ, Lannin DR, Herbst RS, Chen S
  TITLE     Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo
            CRISPR Screens in CD8 T Cells.
  JOURNAL   Cell. 2019 Aug 22;178(5):1189-1204.e23. doi: 
            10.1016/j.cell.2019.07.044.
  PUBMED    31442407
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.07"; journalName: "Cell"; date: "2019-08-22- 
            22"; volume: "178"; issue: "5"; pages: "1189-1204.e23"
COMMENT     Sequence Label: pRC55-GFP-DHX37
FEATURES             Location/Qualifiers
     source          1..11941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2869..2878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2875..2877,2878..2880,2881..3591)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2920..2941)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3181..3200)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3528..3549
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7057..7077
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7078..7095
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7096..7161
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7162..7188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7189..7212
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7228..7281
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7282..7680
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7746..7926
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7952..7969)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7958..8182
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8228..8656
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8315..8334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8525..8546
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8665..8685)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8670..8999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8850..8985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8912..8931
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9047..9094
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9113..9487
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9617..9738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9654..9673)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9708..9727
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9787..9803)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9787..9803)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9800..9822)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9811..9827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9835..9841,9842..9859,9860..9865))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9880..9901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10018..10035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10189..10777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10269..10288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10948..11739,11740..11808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11571..11590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11809..11913)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gggaattcgc caccatggga
     2881 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2941 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3001 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3061 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3121 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3181 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3241 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3301 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3361 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3421 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3481 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3541 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggtaccggt
     3601 ggaagcatgg ggaagctgcg acgccgctac aacgtgaagg ggcggctgca ggccgatccc
     3661 cggcccgcca agggccctga agcgcctccc gtacggctgg aactggaggg caaggatgtg
     3721 ttgaaaggag ttgatgaaag caatgttctg gtgctgcctg ggaagagaaa gaagaagacc
     3781 aaggcgccgc ccccatccaa gaaagagagg aagcccctga caaagaagga gaggaaggtg
     3841 ctacagaagg tcttagaaca gaaggagaag aagagccagc gtgccgaact gctgcagaaa
     3901 ctgagtgaag tccaagtatc ggaggccgag atgagtctct tctacaccac agccaagctc
     3961 ggcactgggg accgcatgta tcatatcaaa gagaggaagt ctggccagcc tgctgcccgg
     4021 ggccaggaga aggtcagtag cctcagcggg gcccaccgga agcgccacag gtcatcatca
     4081 acagaggaag actctgagtc ttcagaagat tcagagcctg aggaagccac agctgaccaa
     4141 cctaggacca gcacagggac agagccagtg catccgccac cagctccttc tgggatcaac
     4201 agccctgcac ccaccccgca gccccctcca tctgggatct ctgcacctcc aaaaacacca
     4261 gcctctgccc cgcctccacc cgtggccaag cctgctgtat tcatccccgt gaaccgcacc
     4321 cctgaaatgc aggaggagcg gctcaagctc ccgatcctcg ccgaggagca agccatcatg
     4381 gaggcggtgg ctgagcaccc cattgtcatt gtgtgtggcg agactggcag tgggaagact
     4441 acacaggtgc cccagtttct ctacgaagca ggctacagca gtgaggacag catcatcggt
     4501 gtcacagaac cccgccgagt ggccgcagta gccatgtccc agcgggtggc caaggagatg
     4561 aacctgtcac atcgggttgt ctcctaccag atccgctacg aagggaatgt gacagaagaa
     4621 accaggatca agttcatgac ggacggtgtg ctgctcaaag agatccagaa ggacttcctg
     4681 ctgctgaaat acaaggtggt gatcattgac gaagcccatg agcggagcgt ctacacagac
     4741 atccttctag ggctcctgtc ccgcattgtg gctctccggg ccaagaggca cctgcccctc
     4801 aagctgctca tcatgtcggc cacactgcgg gtggaggact tcacccagaa ccagcggctc
     4861 ttcaccaccc ctcccccggt catcaaggtt gagtcgaggc agttccctgt gactgtacat
     4921 ttcaacaagc ggaccccatt ggatgactac agtggtgaat gcttcaggaa ggtctgcaag
     4981 atccaccgga tgctgcctgc aggtggcatc ctggtatttc tcacagggca ggccgaggtg
     5041 catgcccttt gccgtcggct taggaaggcc ttcccctttc gttgctccca gccacaagaa
     5101 aaggaagagg actcagcaga agggatgcgg aggtttaaga agtcccggac aagggctagg
     5161 aaggcccagg ccatggcctt gccccagatc aacctggaca attactctgt gctgccagcc
     5221 ggcgaaggtg atgaggacag ggaggcagag atggacgatg aagaggaggc cctgggctct
     5281 gacctagatc tggacctggg tgacagtgag gcaaatgaag gtgagcagcc ggatgcctcc
     5341 ctgccccttc acgtgctccc gctctactct ttgctggccc ctgagaagca ggcacaggtc
     5401 ttcaaacctc ctcccgaggg gacgaggctg tgcgttgtgg ccaccaatgt ggctgaaacc
     5461 tccctcacca tccctggtat caagtatgtg gtggactgtg ggaaagtcaa aaagcgctat
     5521 tatgatcgtg tcacaggcgt gtcttccttc cgtgtcacct gggtctccca agcatccgct
     5581 gaccagcggg ctggtcgtgc aggccgcaca gagccaggcc actgctatag gctgtattcc
     5641 tcagctgttt tcggggactt tgagcagttt cctcctccag agatcacccg cagaccagtg
     5701 gaggacttga tcctgcaaat gaaagcactc agcattgaga aggtcatcaa cttcccgttc
     5761 cccaccccgc cgtccgtgga ggccctcgtt gctgcagagg agctgctggt tgccctgggg
     5821 gccctgcagg cacctccaaa acaggaaaga atgaagaagc tacagatgtc acagctgagc
     5881 tgccccatca ccgcgctggg gcggaccatg tccaccttcc ctgtggcacc ccgctatgcc
     5941 aagatgctgg ccctgagcca gcagcatggc tgcctgccct acaccattgc catcgtggct
     6001 gccatgactg tgcgtgagct gtttgaggag ctggacagac ctgctgccag tgaaaaggag
     6061 cttgccgagc taaagggccg ccgagcccgt gtggcccaga tgaagaggac ctgggccggg
     6121 caggggccat ctctgaagct tggcgacctc atggtgttgc tgggtgccgt gggagcctgt
     6181 gagtacgctg gctgctcacc ccagttctgc caagctaacg gactgcgcta caaggccatg
     6241 cttgagatcc ggcgcctgcg tggccagctg accactgcag tcaacgcagt gtgccccgag
     6301 gctgggcttt tcctggaccc caaaatgcag cctcctactg agagccaggt gacttacctg
     6361 cggcaaatca tggcagctgg tctgggtgac cacctggccc gccgggttca gagtgaggac
     6421 ttgctggacc ccaagtggaa gaatgcctac aagactcctc ttctagatga ccccgtcttc
     6481 atccacccca gctctgtact cttcaaagag ttgcctgagt tcgtggtcta ccaggaaatt
     6541 gtggagacaa ccaagatgta catgaaaggt gtctccaccg tggaaatcca gtggatcccc
     6601 tccctgctgc cttcctactg ccagtttgat gcacccctgg aggagccagc gcccagttac
     6661 tgcccagagt caggacaggt gttgtgccac cgggccagcg tgttctaccg tgtgggctgg
     6721 cctctccctg ccgtccaggt ggacttccct gaaggcattg accgctacaa atactttgca
     6781 aagtttctgc tggagggaca ggtcttccgc aagctggctt cattcaagag ctgcctgcta
     6841 tctagcccca gcaccatgct gaagacctgg gccaggctgc agcccaggac agagacccta
     6901 ttgagagccc tcgtggccca caaggctgat tctcgtgatt ccctgctggc tgcatggaag
     6961 aagaacccca aatacctgct ggctgagtac tgcgagtggc tcccaaaggc tatgcacagc
     7021 gatgttgaga agaactggcc ccctaccact gacagtgaga acctgtactt ccagggccat
     7081 catcatcatc atcatgacta caaagaccat gacggtgatt ataaagatca tgacatcgat
     7141 tacaaggatg acgatgacaa gtacccatac gatgttccag attacgcttg gagccacccc
     7201 cagttcgaga agtgtacagg cagtggagag ggcagaggaa gtctgctaac atgcggtgac
     7261 gtcgaggaga atcctggccc aatggccaag cctttgtctc aagaagaatc caccctcatt
     7321 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     7381 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     7441 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     7501 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     7561 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     7621 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     7681 gaattaataa aagatcttta ttttcattag atctgtgtgt tggttttttg tgtccggact
     7741 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7801 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7861 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7921 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     7981 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8041 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8101 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8161 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     8221 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8281 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8341 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8401 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8461 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8521 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8581 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8641 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     8701 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     8761 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8821 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8881 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     8941 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9001 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     9061 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     9121 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     9181 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     9241 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     9301 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     9361 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     9421 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     9481 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9541 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9601 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9661 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9721 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     9781 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9841 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9901 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9961 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10021 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10081 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10141 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10201 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10261 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10321 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10381 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10441 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10501 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10561 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10621 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10681 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10741 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10801 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10861 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10921 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10981 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11041 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11101 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11161 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11221 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11281 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11341 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11401 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11461 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11521 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11581 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11641 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11701 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11761 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11821 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11881 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11941 c
//
