LOCUS       sequence-375235-       11887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express DHX37-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Dong MB, Wang G, Chow RD, Ye L, Zhu L, Dai X, Park JJ, Kim HR, 
            Errami Y, Guzman CD, Zhou X, Chen KY, Renauer PA, Du Y, Shen J, Lam 
            SZ, Zhou JJ, Lannin DR, Herbst RS, Chen S
  TITLE     Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo
            CRISPR Screens in CD8 T Cells.
  JOURNAL   Cell. 2019 Aug 22;178(5):1189-1204.e23. doi: 
            10.1016/j.cell.2019.07.044.
  PUBMED    31442407
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.07"; journalName: "Cell"; date: "2019-08-22- 
            22"; volume: "178"; issue: "5"; pages: "1189-1204.e23"
COMMENT     Sequence Label: pRC54-DHX37-GFP
FEATURES             Location/Qualifiers
     source          1..11887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6337..6357
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6358..6375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6391..7101
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(6430..6451)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6691..6710)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7038..7059
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7108..7134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7174..7227
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7228..7626
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7692..7872
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7898..7915)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7904..8128
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8174..8602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8261..8280)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8471..8492
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8611..8631)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8616..8945
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8796..8931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8858..8877
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8993..9040
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9059..9433
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9563..9684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9600..9619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9654..9673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9733..9749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9733..9749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9746..9768)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9757..9773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9781..9787,9788..9805,9806..9811))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9826..9847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9964..9981)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10135..10723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10215..10234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10894..11685,11686..11754))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11517..11536
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11755..11859)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagacgtacg
     2881 agcgctatgg ggaagctgcg acgccgctac aacgtgaagg ggcggctgca ggccgatccc
     2941 cggcccgcca agggccctga agcgcctccc gtacggctgg aactggaggg caaggatgtg
     3001 ttgaaaggag ttgatgaaag caatgttctg gtgctgcctg ggaagagaaa gaagaagacc
     3061 aaggcgccgc ccccatccaa gaaagagagg aagcccctga caaagaagga gaggaaggtg
     3121 ctacagaagg tcttagaaca gaaggagaag aagagccagc gtgccgaact gctgcagaaa
     3181 ctgagtgaag tccaagtatc ggaggccgag atgagtctct tctacaccac agccaagctc
     3241 ggcactgggg accgcatgta tcatatcaaa gagaggaagt ctggccagcc tgctgcccgg
     3301 ggccaggaga aggtcagtag cctcagcggg gcccaccgga agcgccacag gtcatcatca
     3361 acagaggaag actctgagtc ttcagaagat tcagagcctg aggaagccac agctgaccaa
     3421 cctaggacca gcacagggac agagccagtg catccgccac cagctccttc tgggatcaac
     3481 agccctgcac ccaccccgca gccccctcca tctgggatct ctgcacctcc aaaaacacca
     3541 gcctctgccc cgcctccacc cgtggccaag cctgctgtat tcatccccgt gaaccgcacc
     3601 cctgaaatgc aggaggagcg gctcaagctc ccgatcctcg ccgaggagca agccatcatg
     3661 gaggcggtgg ctgagcaccc cattgtcatt gtgtgtggcg agactggcag tgggaagact
     3721 acacaggtgc cccagtttct ctacgaagca ggctacagca gtgaggacag catcatcggt
     3781 gtcacagaac cccgccgagt ggccgcagta gccatgtccc agcgggtggc caaggagatg
     3841 aacctgtcac atcgggttgt ctcctaccag atccgctacg aagggaatgt gacagaagaa
     3901 accaggatca agttcatgac ggacggtgtg ctgctcaaag agatccagaa ggacttcctg
     3961 ctgctgaaat acaaggtggt gatcattgac gaagcccatg agcggagcgt ctacacagac
     4021 atccttctag ggctcctgtc ccgcattgtg gctctccggg ccaagaggca cctgcccctc
     4081 aagctgctca tcatgtcggc cacactgcgg gtggaggact tcacccagaa ccagcggctc
     4141 ttcaccaccc ctcccccggt catcaaggtt gagtcgaggc agttccctgt gactgtacat
     4201 ttcaacaagc ggaccccatt ggatgactac agtggtgaat gcttcaggaa ggtctgcaag
     4261 atccaccgga tgctgcctgc aggtggcatc ctggtatttc tcacagggca ggccgaggtg
     4321 catgcccttt gccgtcggct taggaaggcc ttcccctttc gttgctccca gccacaagaa
     4381 aaggaagagg actcagcaga agggatgcgg aggtttaaga agtcccggac aagggctagg
     4441 aaggcccagg ccatggcctt gccccagatc aacctggaca attactctgt gctgccagcc
     4501 ggcgaaggtg atgaggacag ggaggcagag atggacgatg aagaggaggc cctgggctct
     4561 gacctagatc tggacctggg tgacagtgag gcaaatgaag gtgagcagcc ggatgcctcc
     4621 ctgccccttc acgtgctccc gctctactct ttgctggccc ctgagaagca ggcacaggtc
     4681 ttcaaacctc ctcccgaggg gacgaggctg tgcgttgtgg ccaccaatgt ggctgaaacc
     4741 tccctcacca tccctggtat caagtatgtg gtggactgtg ggaaagtcaa aaagcgctat
     4801 tatgatcgtg tcacaggcgt gtcttccttc cgtgtcacct gggtctccca agcatccgct
     4861 gaccagcggg ctggtcgtgc aggccgcaca gagccaggcc actgctatag gctgtattcc
     4921 tcagctgttt tcggggactt tgagcagttt cctcctccag agatcacccg cagaccagtg
     4981 gaggacttga tcctgcaaat gaaagcactc agcattgaga aggtcatcaa cttcccgttc
     5041 cccaccccgc cgtccgtgga ggccctcgtt gctgcagagg agctgctggt tgccctgggg
     5101 gccctgcagg cacctccaaa acaggaaaga atgaagaagc tacagatgtc acagctgagc
     5161 tgccccatca ccgcgctggg gcggaccatg tccaccttcc ctgtggcacc ccgctatgcc
     5221 aagatgctgg ccctgagcca gcagcatggc tgcctgccct acaccattgc catcgtggct
     5281 gccatgactg tgcgtgagct gtttgaggag ctggacagac ctgctgccag tgaaaaggag
     5341 cttgccgagc taaagggccg ccgagcccgt gtggcccaga tgaagaggac ctgggccggg
     5401 caggggccat ctctgaagct tggcgacctc atggtgttgc tgggtgccgt gggagcctgt
     5461 gagtacgctg gctgctcacc ccagttctgc caagctaacg gactgcgcta caaggccatg
     5521 cttgagatcc ggcgcctgcg tggccagctg accactgcag tcaacgcagt gtgccccgag
     5581 gctgggcttt tcctggaccc caaaatgcag cctcctactg agagccaggt gacttacctg
     5641 cggcaaatca tggcagctgg tctgggtgac cacctggccc gccgggttca gagtgaggac
     5701 ttgctggacc ccaagtggaa gaatgcctac aagactcctc ttctagatga ccccgtcttc
     5761 atccacccca gctctgtact cttcaaagag ttgcctgagt tcgtggtcta ccaggaaatt
     5821 gtggagacaa ccaagatgta catgaaaggt gtctccaccg tggaaatcca gtggatcccc
     5881 tccctgctgc cttcctactg ccagtttgat gcacccctgg aggagccagc gcccagttac
     5941 tgcccagagt caggacaggt gttgtgccac cgggccagcg tgttctaccg tgtgggctgg
     6001 cctctccctg ccgtccaggt ggacttccct gaaggcattg accgctacaa atactttgca
     6061 aagtttctgc tggagggaca ggtcttccgc aagctggctt cattcaagag ctgcctgcta
     6121 tctagcccca gcaccatgct gaagacctgg gccaggctgc agcccaggac agagacccta
     6181 ttgagagccc tcgtggccca caaggctgat tctcgtgatt ccctgctggc tgcatggaag
     6241 aagaacccca aatacctgct ggctgagtac tgcgagtggc tcccaaaggc tatgcacagc
     6301 gatgttgaga agaactggcc ccctaccact gacagtgaga acctgtactt ccagggccat
     6361 catcatcatc atcatgaatt cggttctggt agcaagggcg aggagctgtt caccggggtg
     6421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     6481 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     6601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6661 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6781 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     6841 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     6901 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     6961 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     7021 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     7081 ggcatggacg agctgtacaa gggtacctac ccatacgatg ttccagatta cgcttggagc
     7141 cacacccagt tcgagaagtg tacaggcagt ggagagggca gaggaagtct gctaacatgc
     7201 ggtgacgtcg aggagaatcc tggcccaatg gccaagcctt tgtctcaaga agaatccacc
     7261 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     7321 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     7381 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     7441 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     7501 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     7561 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     7621 ggctaagaat taataaaaga tctttatttt cattagatct gtgtgttggt tttttgtgtc
     7681 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7741 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7801 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7861 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     7921 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7981 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8041 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8101 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     8161 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8521 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8581 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8701 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8761 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8821 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8881 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8941 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     9001 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9061 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     9121 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     9181 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     9241 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     9301 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     9361 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     9421 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9481 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9541 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9601 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9661 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9721 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9781 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9841 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9901 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9961 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10021 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10081 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10141 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10201 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10261 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10321 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10381 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10441 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10501 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10561 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10621 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10681 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10741 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10801 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10861 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10921 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10981 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    11041 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    11101 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    11161 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    11221 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11281 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11341 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11401 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11461 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11521 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11581 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11641 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11701 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11761 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11821 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11881 acctgac
//
