LOCUS       sequence-375243-       13579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-XRCC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13579)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 13579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-XRCC6 zeo
FEATURES             Location/Qualifiers
     source          1..13579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2993..3058
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4930..4954)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5004..6215
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6052..6071
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6274..6891,6892..6897,6898..6933,6934..6936,6937..6972,
                     6973..6975,6976..7011,7012..7020)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7058..7631
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7225..7242)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7452..7471
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        7653..7700
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7719..8093
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8267..8855
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8320..8340)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8738..8749)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8858..8874)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8859..8875)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9384..9564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9590..9607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9596..9820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9866..10294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9953..9972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10163..10184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10303..10323)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10308..10637
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10488..10623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10550..10569
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10685..10732
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10751..11125
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11255..11376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11292..11311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11346..11365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11425..11441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11425..11441)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11438..11460)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11449..11465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11473..11479,11480..11497,11498..11503))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11518..11539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11656..11673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11827..12415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11907..11926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12586..13377,13378..13446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13209..13228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13447..13551)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga tggactacaa
     3001 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagac
     3061 tagtatgtca gggtgggagt catattacaa aaccgagggc gatgaagaag cagaggaaga
     3121 acaagaagag aaccttgaag caagtggaga ctataaatat tcaggaagag atagtttgat
     3181 ttttttggtt gatgcctcca aggctatgtt tgaatctcag agtgaagatg agttgacacc
     3241 ttttgacatg agcatccagt gtatccaaag tgtgtacatc agtaagatca taagcagtga
     3301 tcgagatctc ttggctgtgg tgttctatgg taccgagaaa gacaaaaatt cagtgaattt
     3361 taaaaatatt tacgtcttac aggagctgga taatccaggt gcaaaacgaa ttctagagct
     3421 tgaccagttt aaggggcagc agggacaaaa acgtttccaa gacatgatgg gccacggatc
     3481 tgactactca ctcagtgaag tgctgtgggt ctgtgccaac ctctttagtg atgtccaatt
     3541 caagatgagt cataagagga tcatgctgtt caccaatgaa gacaaccccc atggcaatga
     3601 cagtgccaaa gccagccggg ccaggaccaa agccggtgat ctccgagata caggcatctt
     3661 ccttgacttg atgcacctga agaaacctgg gggctttgac atatccttgt tctacagaga
     3721 tatcatcagc atagcagagg atgaggacct cagggttcac tttgaggaat ccagcaagct
     3781 agaagacctg ttgcggaagg ttcgcgccaa ggagacaagg aagcgagcac tcagcaggtt
     3841 aaagctgaag ctcaacaaag atatagtgat ctctgtgggc atttataatc tggtccagaa
     3901 ggctctcaag cctcctccaa taaagctcta tcgggaaaca aatgaaccag tgaaaaccaa
     3961 gacccggacc tttaatacaa gtacaggcgg tttgcttctg cctagcgata ccaagaggtc
     4021 tcagatctat gggagtcgtc agattatact ggagaaagag gaaacagaag agctaaaacg
     4081 gtttgatgat ccaggtttga tgctcatggg tttcaagccg ttggtactgc tgaagaaaca
     4141 ccattacctg aggccctccc tgttcgtgta cccagaggag tcgctggtga ttgggagctc
     4201 aaccctgttc agtgctctgc tcatcaagtg tctggagaag gaggttgcag cattgtgcag
     4261 atacacaccc cgcaggaaca tccctcctta ttttgtggct ttggtgccac aggaagaaga
     4321 gttggatgac cagaaaattc aggtgactcc tccaggcttc cagctggtct ttttaccctt
     4381 tgctgatgat aaaaggaaga tgccctttac tgaaaaaatc atggcaactc cagagcaggt
     4441 gggcaagatg aaggctatcg ttgagaagct tcgcttcaca tacagaagtg acagctttga
     4501 gaaccccgtg ctgcagcagc acttcaggaa cctggaggcc ttggccttgg atttgatgga
     4561 gccggaacaa gcagtggacc tgacattgcc caaggttgaa gcaatgaata aaagactggg
     4621 ctccttggtg gatgagttta aggagcttgt ttacccacca gattacaatc ctgaagggaa
     4681 agttaccaag agaaaacacg ataatgaagg ttctggaagc aaaaggccca aggtggagta
     4741 ttcagaagag gagctgaaga cccacatcag caagggtacg ctgggcaagt tcactgtgcc
     4801 catgctgaaa gaggcctgcc gggcttacgg gctgaagagt gggctgaaga agcaggagct
     4861 gctggaagcc ctcaccaagc acttccagga ctgacaattg gaagactaat gcgttttcga
     4921 gatatctaga cccagctttc ttgtacaaag tggttgataa catctcgagc cataaagatg
     4981 gttaattaac ccacccaaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     5041 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     5101 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     5161 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     5221 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     5281 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     5341 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     5401 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     5461 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     5521 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     5581 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     5641 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     5701 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     5761 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     5821 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     5881 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     5941 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     6001 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     6061 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     6121 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     6181 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttggtaccga gctcggatct
     6241 ccaccccgta ccggtcctgc agtcgaattc accatgtcta gactggacaa gagcaaagtc
     6301 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     6361 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     6421 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     6481 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     6541 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     6601 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     6661 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     6721 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     6781 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     6841 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     6901 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     6961 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     7021 ctaagtaagg atccgcggcc gcactagagg aattccgccc ctctcccccc ccccccccta
     7081 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     7141 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     7201 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     7261 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     7321 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     7381 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     7441 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     7501 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     7561 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     7621 cacgatgata agcttaccgg tacgcgtgat gtgttgacaa ttaatcatcg gcatagtata
     7681 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     7741 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     7801 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     7861 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     7921 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     7981 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     8041 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacatct
     8101 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     8161 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     8221 attgttgttg tttagtccct cccaattcga tatcaagctt atcgataatc aacctctgga
     8281 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8341 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8401 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8461 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8521 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8581 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8641 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     8701 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8761 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8821 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     8881 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     8941 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     9001 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9061 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     9121 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     9181 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     9241 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     9301 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     9361 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9541 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     9601 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     9661 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     9721 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     9781 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     9841 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9901 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9961 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10021 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10081 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10141 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    10201 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    10261 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    10321 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10381 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10441 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10501 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10561 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10621 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    10681 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
    10741 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    10801 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    10861 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    10921 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    10981 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    11041 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    11101 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    11161 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    11221 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    11281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11341 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    11401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    11461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    12301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    13021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13561 ccgaaaagtg ccacctgac
//
