LOCUS       sequence-37525-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Duxin JP, Dao B, Martinsson P, Rajala N, Guittat L, Campbell JL, 
            Spelbrink JN, Stewart SA
  TITLE     Human Dna2 is a nuclear and mitochondrial DNA maintenance protein.
  JOURNAL   Mol Cell Biol. 2009 Aug;29(15):4274-82. Epub 2009 Jun 1.
  PUBMED    19487465
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2009-08"; volume: "29(15)"; pages: "4274-82. Epub 
            2009 Jun 1"
COMMENT     Sequence Label: pLKO.1p shDna2
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    133..250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        299..809
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     728..746
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ttctggcttt tatatcttgt ggaaggacga aacaccggca tagccagtag tattcgatgc
       61 tcgagcatcg aatactactg gctatgtttt tgaattctcg acctcgagac aaatggcagt
      121 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      181 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      241 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg
      301 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
      361 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
      421 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
      481 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
      541 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
      601 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
      661 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
      721 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcantc ggctccctcg
      781 ttgaccgaat caccgacctc tctcccnagg gggatncacc ggagcttacc atgac
//
