LOCUS       sequence-37527-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz 
            J, Verkhusha VV
  TITLE     Photoactivatable mCherry for high-resolution two-color fluorescence 
            microscopy.
  JOURNAL   Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25.
  PUBMED    19169259
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-02"; volume: "6(2)"; pages: "153-9. Epub 2009
            Jan 25"
COMMENT     Sequence Label: pTfR-PAmCherry1
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(128..838)
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     675..693
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      835..844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
      121 ggccgcttta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggctctct
      181 cgtactgctc cacgatggtg tagtcctcgt tgtggctggt gatgtccagc ttgcggttga
      241 cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag
      301 cgtcgtagtg gccgccgtcc ttcagcttca ctctgggctt gacctcgccc ttcagggcgc
      361 cgtcctcggg gtacatccgc tcggagaggg cctcccagcc catggtcttc ttctgcatca
      421 cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt
      481 cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaat ttcatcacgc
      541 gctcccactt gaagccctcg gggaaggaca gcttaaagta gtcggggatg tcggcggggt
      601 gcttcacgta ggcattggag ccgtacatga actgagggct caggatgtcc caggtgaagg
      661 gcagggggcc gcccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc
      721 cctcgccctc gccctcgatc tcgaacacgt ggccgttcac ggacccctcc atgtgcacct
      781 tgaagcgcat gaactcctta atgatggcca tgttatcctc ctcgcccttg ctcaccatgg
      841 tggcg
//
