LOCUS       sequence-375273-        8454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2s-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans 
            A/B MN expression of GtACR2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH4211
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(2677..2710)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      3587..3910
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(3914..3947)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6657..6679)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6779..6798
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6876..6894)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6962..7066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7067..7135,7136..7927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7285..7304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8098..8454,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
      661 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
      721 atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg ggctgcagca
      781 tgcggccgct caccaccgac tgattttcat ttcaaaatta atgcagattt ctacgtgttc
      841 tttttcaaat aaatttatat attttttgtt ctctcacaaa ataaataatt tcgagagtta
      901 agtgatcatt ctcagacata ttctataatt gtccgtcagg aaacagatag aggaaccttt
      961 gataaataaa ttataaaagg ccgacaggga aatgcttctc cctcgactct gttagaagcg
     1021 aagaggcaat ttttaaaaat ttcccatttt tatgcgtttt tctgatttcc aaagtcacgc
     1081 atcatttacc aaccgcaacg attcataagg acgtaatttt gattttctgg acgattttta
     1141 tatagttttg atgtgaaatt cgctttattt gtctgaaagt ggaatagatt tagtgtggat
     1201 ttaaccgaaa aatgcagatc ttttaaaaat ttaaaccagt gttttgttgc cttatttgcg
     1261 aaaaaaaatt gtgatatttc gagtattgct gcaatttgat tagtaaaatg ttaggatata
     1321 agtatttaaa aagtataatt ggttgaattt tctttttcat caccgacttc caaaaatata
     1381 gagatactga gttttcacca ttttttgaat gtaaataaaa acattttgaa cacttgtgct
     1441 gggattcaat ccaaatacat tcactgtcat ttaatattat tatatatcaa aataaaacgc
     1501 ataacttttt ctgttttttt ttaaattttt tcaaaatgta ggtttggtaa gttaaggtaa
     1561 ggttaggcaa tgtaggtaaa cacgagatga tactccacgt tgaaaattgt tttcataaat
     1621 acagtttaga ttgaactgaa ggattttatc gtctaataga atataatttt ttagttcaag
     1681 atttaggatt tgttttcact tttttttaga ttcaagacat atatctcgca aaatgagttt
     1741 tgtctttgag ccatcttgtc atcatagcac attgaatttt gttgttcgca agacggaata
     1801 tatgttccca gctgcaaatt ttgtgtcgga tatttcttct ttagcgaaag gcgttaaaaa
     1861 ccaaaaatag tcgaagcgtt tgatatgtgt cccgttggtt tttcgtgtcc atctattctg
     1921 tttcgagtac acatgttgcg acgcggcact gatgaaaaag tgaaagagct agtgtcacac
     1981 acaaacacaa agtatgctca tgtgagtgtg tagatatgtg tagatgtatg tcctgaagga
     2041 ataaaaagac tttttgaagc tacattttta aacaaaaaaa gacagaaaca tgaagcacaa
     2101 ctctctattc tgtcgaacgg cgtgtgcaac tcacgcacac ccaaaaatat gaaatataac
     2161 attcgcacca acaaagcgcg aatatcaatg aatctcgagg gaacgatggc tgcctgccgg
     2221 cgcgtgttgc tcttctgtca ttgggataac tggcgggtct gttgggtccg tcctttagac
     2281 catttgacta cactgccctg catatgtgtg aaacgtcgtc gttgctgttg ggagctgtct
     2341 gtgtgtatgt gtgtgtgtgt gagtatggag cagtaaaaag ctgccgatac gagaagcgcc
     2401 gccccggacg gacggcgatg gcgcatcgcc aaatgtgtca ttttagttgt cttcacgaac
     2461 cagagggccc acgacggacg ggcggccacg cccacctcaa ccagaatatc cttccttctc
     2521 ccacccaccc ttgcggacgc gcggatgcgc tgaacaaaaa gacgacgaag aagaagaaga
     2581 agaagctgaa cgaagaaaaa agcatcaaaa gaagtgaagc ggctctacca ccccgaccac
     2641 cttccttcac aggaaggacg ccgttttcat cccgggataa cttcgtatag catacattat
     2701 acgaagttat atggtctcca agggagagga gctcttcacc ggagtcgtcc caatcctcgt
     2761 cgagctcgac ggagacgtca acggacacaa gttctccgtc cgtggagagg gagagggaga
     2821 cgccaccaac ggaaagctca ccctcaagtt catctgcacc accggaaagc tcccagtccc
     2881 agtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agtggccaac
     2941 cctcgtcacc accctctccc acggagtcca gtgcttcgcc cgttacccag accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc agagggatac gtccaggagc gtaccatctt
     3061 cttcaaggac gacggaacct acaagagtaa gtttaaacat gattttacta actaactaat
     3121 ctgatttaaa ttttcagccc gtgccgaggt caagttcgag ggagacaccc tcgtcaaccg
     3181 tatcgagctc aagggagtcg acttcaagga ggacggaaac atcctcggac acaagctcga
     3241 gtacaacttc aactcccaca acatctacat catggccgtc aagcagaaga acggaatcaa
     3301 ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc aggtcaactt
     3361 caagatccgt cacaacgtcg aggacggatc cgtccagctc gccgaccact accagcagaa
     3421 caccccaatc ggagacggac cagtcctcct cccagactcc cactacctct ccacccagtc
     3481 cgtcctctcc aaggacccaa acgagaagcg tgaccacatg gtcctcctcg agttccgtac
     3541 cgccgccgga atcaccctcg gaatggacga gctctacaag tgataaatgc aaaatccttt
     3601 caagcattcc cttcttctct atcactcttc tttctttttg tcaaaaaatt ctctcgctaa
     3661 tttatttgct tttttaatgt tattatttta tgacttttta tagtcactga aaagtttgca
     3721 tctgagtgaa gtgaatgcta tcaaaatgtg attctgtctg atgtactttc acaatctctc
     3781 ttcaattcca ttttgaagtg ctttaaaccc gaaaggttga gaaaaatgcg agcgctcaaa
     3841 tatttgtatt gtgttcgttg agtgacccaa caaaaagagg aaactttatt gtgccgccaa
     3901 gaaaaaagtc tcaataactt cgtatagcat acattatacg aagttatgct agcatggcct
     3961 cccaggtcgt gtacggcgag tgggccagca cccacacaga gtgctacaac atgagcagga
     4021 tcgacagcac cttcgtgagc ctgctgcagc tggtgtgggc cgtggtgagc ggatgccaga
     4081 ccatcttcat gatcagcagg gcccccaagg tgccctggga gagcgtgtac ctgcccttcg
     4141 tggagtccat cacatacgcc ctggccagca ccggcaatgg cacactgcag atgagggacg
     4201 gcaggttctt tccctggtcc aggatggcca gctggctgtg cacatgcccc attatgctgg
     4261 gccagatctc caacatggct ctggtgaagt acaagagcat ccctctgaac cccatcgccc
     4321 aggccgccag cattatcagg gtggtgatgg gcattaccgc taccatctcc cccgccgagt
     4381 acatgaagtg gctgttcttc ttcttcggcg ctacctgcct ggtcttcgaa tacagcgtgg
     4441 tgttcaccat cttccaggtg ggactgtacg gcttcgagtc cgtgggcacc cctctggccc
     4501 agaaggtggt ggtgaggatc aagatgctga ggctgatctt cttcatcgcc tggaccatgt
     4561 tccccatcgt gtggctgatt tcccccaccg gcgtgtgcgt gatccacgag aacgtgagcg
     4621 ccatcctgta tctgctcgcc gacggcctgt gcaagaacac ctacggcgtg atcctgtggt
     4681 ccaccgcctg gggagtgctc gagggaaagt gggacccggc ttgcctccct ggccaggaga
     4741 agcctgaggc cgacgacccc ttcggactga accacgagaa gaatgccccc cccaacgacg
     4801 aggtcaacat cagaatgttc ggcagggcgg ccgccgcggt accgggagga ggaaccatgg
     4861 tctcaaaggg tgaagaagat aacatggcaa ttattaaaga gtttatgcgt ttcaaggtgc
     4921 atatggaggg atctgtcaat gggcatgagt ttgaaattga aggtgaagga gaaggccgac
     4981 catatgaggg aacacaaacc gcaaaactaa aggtaagttt aaacatatat atactaacta
     5041 accctgatta tttaaatttt caggtaacta aaggcggacc attaccattc gcctgggaca
     5101 tcctctctcc acagttcatg tatggaagta aagcttatgt taaacatccg gcagatatac
     5161 cagattattt gaaactttca ttcccggagg gttttaagtg ggaacgcgta atgaattttg
     5221 aagacggagg agttgttaca gtgacgcaag actcaaggta agtttaaaca gttcggtact
     5281 aactaaccat acatatttaa attttcagcc tccaagatgg agaatttatt tataaagtca
     5341 aacttcgagg aacgaatttc ccctcggatg gatctgttat gcagaagaag actatgggat
     5401 gggaagcttc aagtgaaaga atgtaccctg aagacggtgc tcttaaggga gagattaaac
     5461 aacgtcttaa attgaaagat ggaggacatt acgatgctga ggtaagttta aacatgattt
     5521 tactaactaa ctaatctgat ttaaattttc aggtgaagac aacttacaaa gccaaaaaac
     5581 cagttcagct gccaggagcg tacaatgtta atattaaact ggatatcacc tcccacaacg
     5641 aggattacac tatcgttgag caatatgaaa gagctgaagg gcggcactcg acaggtggca
     5701 tggatgaatt gtataagttc tgctacgaga acgaggtcta agaattccaa ctgagcgccg
     5761 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     5821 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     5881 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     5941 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     6001 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     6061 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     6121 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     6181 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     6241 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     6301 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     6361 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     6421 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     6481 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     6541 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     6601 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6661 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6721 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     6781 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6841 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     6901 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7021 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7081 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7141 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7201 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7261 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7321 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7381 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7441 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7501 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7561 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7621 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7681 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7741 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7801 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7861 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7921 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaac
//
