LOCUS       sequence-375283-        9399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Psra-11-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans 
            AVB/others neuron expression of GtACR2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH4235
FEATURES             Location/Qualifiers
     source          1..9399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3622..3655)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      4532..4855
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(4859..4892)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7602..7624)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7724..7743
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7821..7839)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7907..8011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8012..8080,8081..8872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8230..8249)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9043..9399,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
      661 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
      721 atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg ggctgcagca
      781 tgcggccgca cgttttccct ttacattagc aaaaccgacc aacgaccgaa acctaagcat
      841 tatccaggcc taaacatatt ttaaaaaaag tgtagtttac caaatgggag acattttcct
      901 tgttctttgt gggatgcttt tgaaattgaa tagaaatctc tagattaatc aatgtgtttg
      961 ctccgatgcg cctagtctca aagccttctc atgttgcaaa caaatgcact aactaaacta
     1021 attaacaagt cacggaggag attcaaaaca tttcactttt caggaaagta gcatttttaa
     1081 atatattcat gttgtagatt tgtcattttg caatattttt tatcaaaatt ggattacacc
     1141 ctcttttgag cagtctgact tgttggctga cttgattttc ttttaaagtg atgaaatttt
     1201 attcatattt gatttttagt aaataaatta ttgcaaaccc cattatgcaa ctttgacagc
     1261 ttatatctca gttgccgttt gttttatgaa aaattgtcaa cttaccaaat aattcttagt
     1321 tacgatctga aatttttcga aaactctaat ctttagtcaa aaatgttcag caatgtaaac
     1381 attttttcat tacaaaaaaa gatgatgtag atgcatacat caagtatctc aaccggaaca
     1441 ccccctattt tgcaccatct ctgtcatctt tttcttcctt tttcagatgg gaaacataca
     1501 attaatgctc acatgtcatg tctagtcatt tgtttttttt tgtttgtttt taatccgaat
     1561 ctcttattca tctttagacg ttccattcgg cagaggcgag ttggggcgcc tcttactcgc
     1621 ctcaaatttt gtgtttgcac tcataatcaa agaataaatt atcttgccca ttttggtcct
     1681 tgaccatttg tgtaggcaat aggcaggcag acatattttc ttaaacagaa gaccgaactg
     1741 tgagtgtaca aaaataaatt aaatattacc tacctttgtt agaatttgga ataagattaa
     1801 aaaattcgtt gtcctctgtg gaattgctca ttattagatt tttacgaaat catgcggatt
     1861 agaaagttta atatttatgt acagtgcgtc caacttctat agcacccccc tccaattcgg
     1921 ccgtttggag cttttcaaaa tatttttcgg aaaagtgtta gtgcagttcg atagcaaata
     1981 ttgaaaaacc tatgctcccc aatttttatc atgatatctc aaaaatcacg gaaattgggt
     2041 caaaatatct gaaaaatggc cgtcgaactt ctatagcacc ccctctgatt tttgacaaat
     2101 ttctgtaaat tcggggtttt cttcgtagtt aatgaattta ttttgtaaat aacttcacac
     2161 caaaataatc aggtggtttc actttcacat ttaaaaaata aatataacac ttgaaaacac
     2221 gtggaatgcc tgggattgat caaaaattga tcaaaatgtt cttctccgtc tattcattac
     2281 tatgtaatta gttgtagaaa gcatttattc atatgaacca aatggtaaag ggagcaactt
     2341 cagtggtttt taaaaggctc gatttaacca tgaaaacgag cgttcggagg cacaaagctg
     2401 aaggttttga cttcgtattg tacagaactt cagctcatcc tgctcaaatg tctttgaagc
     2461 ttcgattttg attagatgtc tttgaaaatt tatacggaaa agatcatgag accgctcaaa
     2521 aagaggacaa aggcaaagcg aggccgcaag tctttttttc aagctaacat gcgaaaattt
     2581 tagacagaaa agtaagatat acctttccaa atgacgattt tgctagttgt atttataact
     2641 caagttaaat ttagcgaatt cgctcattga atcttttttt ggtcaagtaa tgcaaaagct
     2701 accagacaaa acattgaacc tgcgattgta gatgtctggc atctgattta cccatggatt
     2761 tccacggatt ttttgttcat ataaaataat ttttaatttt tgctataatg tagtaatttt
     2821 tagtcgaaaa tgtattccca accattatct ctgcgcctcc gaacgctcgt tttcatggtt
     2881 aaatcgaacc ttttaaaaac cactgaagtt gctcccttta ccatttggtt catatgaata
     2941 aatgctttct acaactaatt atatagtaat gaatagacgg agaagaacat tttgatctat
     3001 tttggtgctg cgaaaatcat tgggagccaa gatatggcct ctgaagcaaa taagaacgtt
     3061 gaggggggtg ctatagaagt tggacgcact gtatttccaa ttatttttta agaatttcga
     3121 ttttataaac agcctttgct ccgcctattt gagctcatga tgtttctctt ttgttttcaa
     3181 gattgttgat tccttcctat tgtcctattg tgatttttaa atttttaaat tgctcattaa
     3241 cttgattttt atttagtaca tatctctaat atgttgtttc acattgcacc aaccggtctc
     3301 aatttttgaa attatttatt gttatgtttg aatattttaa acaaatttaa aaatttagaa
     3361 aaattgctga gctgttgcct aaactcataa gtgtttcaaa atttgctacg attttgcatg
     3421 tactttttaa tattttatat gctttttcgt attccaaaac aaacaattta gtttacttta
     3481 caaattatga acaaaaaaaa tttgatgtaa cggagagaaa attattaaac gttacatgta
     3541 tttttcgatc gtatcgagaa aaatgcaaaa aatatcaaat tgtaattttt ccgcacatct
     3601 ccgcaacaca caattcccgg gataacttcg tatagcatac attatacgaa gttatatggt
     3661 ctccaaggga gaggagctct tcaccggagt cgtcccaatc ctcgtcgagc tcgacggaga
     3721 cgtcaacgga cacaagttct ccgtccgtgg agagggagag ggagacgcca ccaacggaaa
     3781 gctcaccctc aagttcatct gcaccaccgg aaagctccca gtcccagtaa gtttaaacat
     3841 atatatacta actaaccctg attatttaaa ttttcagtgg ccaaccctcg tcaccaccct
     3901 ctcccacgga gtccagtgct tcgcccgtta cccagaccac atgaagcagc acgacttctt
     3961 caagtccgcc atgccagagg gatacgtcca ggagcgtacc atcttcttca aggacgacgg
     4021 aacctacaag agtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc
     4081 agcccgtgcc gaggtcaagt tcgagggaga caccctcgtc aaccgtatcg agctcaaggg
     4141 agtcgacttc aaggaggacg gaaacatcct cggacacaag ctcgagtaca acttcaactc
     4201 ccacaacatc tacatcatgg ccgtcaagca gaagaacgga atcaaggtaa gtttaaacat
     4261 gattttacta actaactaat ctgatttaaa ttttcaggtc aacttcaaga tccgtcacaa
     4321 cgtcgaggac ggatccgtcc agctcgccga ccactaccag cagaacaccc caatcggaga
     4381 cggaccagtc ctcctcccag actcccacta cctctccacc cagtccgtcc tctccaagga
     4441 cccaaacgag aagcgtgacc acatggtcct cctcgagttc cgtaccgccg ccggaatcac
     4501 cctcggaatg gacgagctct acaagtgata aatgcaaaat cctttcaagc attcccttct
     4561 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
     4621 aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa
     4681 tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa ttccattttg
     4741 aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt gtattgtgtt
     4801 cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa aagtctcaat
     4861 aacttcgtat agcatacatt atacgaagtt atgctagcat ggcctcccag gtcgtgtacg
     4921 gcgagtgggc cagcacccac acagagtgct acaacatgag caggatcgac agcaccttcg
     4981 tgagcctgct gcagctggtg tgggccgtgg tgagcggatg ccagaccatc ttcatgatca
     5041 gcagggcccc caaggtgccc tgggagagcg tgtacctgcc cttcgtggag tccatcacat
     5101 acgccctggc cagcaccggc aatggcacac tgcagatgag ggacggcagg ttctttccct
     5161 ggtccaggat ggccagctgg ctgtgcacat gccccattat gctgggccag atctccaaca
     5221 tggctctggt gaagtacaag agcatccctc tgaaccccat cgcccaggcc gccagcatta
     5281 tcagggtggt gatgggcatt accgctacca tctcccccgc cgagtacatg aagtggctgt
     5341 tcttcttctt cggcgctacc tgcctggtct tcgaatacag cgtggtgttc accatcttcc
     5401 aggtgggact gtacggcttc gagtccgtgg gcacccctct ggcccagaag gtggtggtga
     5461 ggatcaagat gctgaggctg atcttcttca tcgcctggac catgttcccc atcgtgtggc
     5521 tgatttcccc caccggcgtg tgcgtgatcc acgagaacgt gagcgccatc ctgtatctgc
     5581 tcgccgacgg cctgtgcaag aacacctacg gcgtgatcct gtggtccacc gcctggggag
     5641 tgctcgaggg aaagtgggac ccggcttgcc tccctggcca ggagaagcct gaggccgacg
     5701 accccttcgg actgaaccac gagaagaatg ccccccccaa cgacgaggtc aacatcagaa
     5761 tgttcggcag ggcggccgcc gcggtaccgg gaggaggaac catggtctca aagggtgaag
     5821 aagataacat ggcaattatt aaagagttta tgcgtttcaa ggtgcatatg gagggatctg
     5881 tcaatgggca tgagtttgaa attgaaggtg aaggagaagg ccgaccatat gagggaacac
     5941 aaaccgcaaa actaaaggta agtttaaaca tatatatact aactaaccct gattatttaa
     6001 attttcaggt aactaaaggc ggaccattac cattcgcctg ggacatcctc tctccacagt
     6061 tcatgtatgg aagtaaagct tatgttaaac atccggcaga tataccagat tatttgaaac
     6121 tttcattccc ggagggtttt aagtgggaac gcgtaatgaa ttttgaagac ggaggagttg
     6181 ttacagtgac gcaagactca aggtaagttt aaacagttcg gtactaacta accatacata
     6241 tttaaatttt cagcctccaa gatggagaat ttatttataa agtcaaactt cgaggaacga
     6301 atttcccctc ggatggatct gttatgcaga agaagactat gggatgggaa gcttcaagtg
     6361 aaagaatgta ccctgaagac ggtgctctta agggagagat taaacaacgt cttaaattga
     6421 aagatggagg acattacgat gctgaggtaa gtttaaacat gattttacta actaactaat
     6481 ctgatttaaa ttttcaggtg aagacaactt acaaagccaa aaaaccagtt cagctgccag
     6541 gagcgtacaa tgttaatatt aaactggata tcacctccca caacgaggat tacactatcg
     6601 ttgagcaata tgaaagagct gaagggcggc actcgacagg tggcatggat gaattgtata
     6661 agttctgcta cgagaacgag gtctaagaat tccaactgag cgccggtcgc taccattacc
     6721 aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt
     6781 tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc
     6841 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     6901 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     6961 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     7021 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     7081 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     7141 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
     7201 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
     7261 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt
     7321 ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca
     7381 caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt
     7441 ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt
     7501 aaatgacaga atacattccc aatataccaa acataactgt ttcctactag tcggccgtac
     7561 gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7621 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7681 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     7741 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     7801 cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7861 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7921 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7981 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8041 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8101 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8161 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8221 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8281 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8341 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8401 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8461 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8521 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8581 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8641 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8701 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8761 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8821 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8881 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8941 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9001 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9061 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9181 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9361 aacggggggt tcgtgcacac agcccagctt ggagcgaac
//
