LOCUS       sequence-375285-        5979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ptwk-40s::Cre unc-54 3' UTR C.elegans for Cre/Lox.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH4237
FEATURES             Location/Qualifiers
     source          1..5979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    377..398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(413..418,419..436,437..443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    451..467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     456..478
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     475..491
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     475..491
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2117..2137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2138..3166
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     primer_bind     complement(4035..4057)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4157..4176
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4258..4276)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4344..4448
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4449..4517,4518..5309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4667..4686)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5480..5979,1..89)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(5969..5979,1..9)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
       61 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      181 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      241 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      301 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      361 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      421 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      481 acagctatga ccatgattac gccaagcttg catgcgcggc cgctctaatc actatcacgt
      541 gggatctgga taaattttct ccaaatttct cataaaaaat ggaaaaaaag aaaaggaaat
      601 ccggtttgtg tatttccatt ttcaatttga tgcattaaac cagaacttcg ttaatttccc
      661 ttctcttctc cctcaagcgt gcacactttt tttagatgag aagggctcta tttcgattgg
      721 ttgtttttgt tttgaaccag attttttttt cacaaaaata aaatttggaa ccttttgcca
      781 gtttaataaa gttttttgtt gggaatgttc ctaaaaattt ttaatttatc taatataaaa
      841 cttgatagaa acattaatta acctgctttt ttgcatttat ttccaactga attaaaataa
      901 atgtgaattt tttttactat gtaaataaat cgaaaattct ggttcctgat tctcaagacc
      961 gcatctcaac ttttcagccg gtaatttgtc cgagaggagt ccaattttca aatttttgat
     1021 caaaatgtct actttgatcg acatcctcct tcttctgcaa ggtgtgtatc atcgtcttga
     1081 aatttcaaat tccctaaggg aacgaaaagc ttgtctcttt tggcagattc gattcgattt
     1141 cacctctttc tcccccctcc tgcctacaat gttgaaaggg tcccctcctt gtgctccaaa
     1201 ttgctcataa actcctccat actggacgct tctttttgcg gaaattggag tcccagatat
     1261 attggaagag ataaacggtt ttcgggtttg cagttgttgc cattttcact gatttttatt
     1321 caagtagtac tacttgaagt tttgttcctg agtaaaattt tataggttcc atgatttatg
     1381 aaattgttct atagtagcac caaacttttc agaacttttt tgtttttttt ttcaatttta
     1441 cagtaaaaag ttattctcaa atgttttctc aatttttgcc ccttattaac tcacgtacgt
     1501 atgtagttta cagagcgtat gtagatgtag ttccagtttt tggcacttat aattttagaa
     1561 gtcgaccgat aataaatgtt taggcatgcc aaatcatcat aagctactca aaaaatagaa
     1621 aatttttgat atgtagcaat tttctaaaac tttatctaaa aatctttttt tccaaaaaat
     1681 gcctttggtt aataaattgt aaatcaaaat tcagcaacaa aaaacctcac tggaaaagtg
     1741 ggagtaaaaa tacgaaataa aattttcgta actttttgtc aaaacagaat cttaaatttc
     1801 ccactatacc catttgtcct tcaaaaatgc catttaaagt aagcaaagag gtatcataaa
     1861 ttctcacaga acgtatgtag tttttgtgtc ctttacttct ccattgcctc atattttcac
     1921 ttttttcgtg gccgcagctc acagtgcaca catcacagaa tgttccagag ccgaatgatt
     1981 ttggatggaa tatcgagtga tgaatattca cccgggattg gccaaaggac ccaaaggtat
     2041 gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg ctagcgtcga
     2101 cggtaccatg ggcgcaccca agaagaagag gaaggtgtcc aatttactga ccgtacacca
     2161 aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga
     2221 catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg
     2281 ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga
     2341 agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca
     2401 gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga
     2461 cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga
     2521 acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat
     2581 ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa
     2641 caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga
     2701 cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt
     2761 agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg
     2821 tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc
     2881 gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca
     2941 tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca
     3001 cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat
     3061 catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga
     3121 tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gattagtcta gaatcactag
     3181 tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tccgcatcgg ccgctgtcat
     3241 cagatcgcca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
     3301 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
     3361 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     3421 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     3481 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
     3541 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     3601 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     3661 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     3721 aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa
     3781 gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga
     3841 aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt
     3901 gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata
     3961 ccaaacataa ctgtttctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     4021 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     4081 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     4141 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4201 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta attaagggcc
     4261 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4321 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4381 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4441 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4501 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4561 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4621 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4681 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4741 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4801 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4861 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4921 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4981 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5041 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5101 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5161 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5221 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5281 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5341 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5401 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5461 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5521 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5581 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5641 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5701 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5761 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5821 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5881 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5941 caggagagcg cacgagggag cttccagggg gaaacgcct
//
