LOCUS       sequence-375287-       11701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prig-3-LoxP::EBFP::LoxP::Chrimson::wCherry unc-54 3' UTR C.elegans 
            AVA neuron expression of Chrimson RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 11701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH4253
FEATURES             Location/Qualifiers
     source          1..11701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5..21
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4983..5016)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      5893..6216
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(6220..6253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6831..6842
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(9291..9313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9413..9432
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9510..9528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9596..9700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9701..9769,9770..10561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9919..9938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10732..11320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11221..11240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11474..11491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11608..11629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11644..11649,11650..11667,11668..11674)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11682..11698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(11687..11701,1..8)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac
       61 taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg
      121 atggatacgc taacaacttg gaaatgaaat aagcttgggc tgcagcatgc ggccgctcgt
      181 ctttaccgct ttgtttttta ttgcttactt tacctctagt gattatctta ttggaattta
      241 tgcacatgac gtgttgtact agtttaacaa tttcaattga cagttttttc gattaggctt
      301 ttgaacatat ttatatattt ctttatttac gaatgtgaac ttaaaagtgg attctcgaaa
      361 tcttttatcg ggttttttga gcctaaaatg acccatttat caagatttat caaaaaagct
      421 atgacggttt aaaggttttt gattaaaggc gtgttttttt tagctaaaat ctcaaactct
      481 gctaaactat cagtgcccca acgtgaaaaa acaacatgaa aaaccccaaa atcaaaaagc
      541 atcttaatca gccaaaaaca cttcataaat tgcaaaaata tgcattaaca aatgacaact
      601 aaatggaaaa gtactcccga cttttcaaga tggaaaaatg tgagatcttc gctgaaaata
      661 gagttttttt tcaaatgaaa aatttggaat aaagctcaaa tgtttgtctc aaagttggta
      721 attgttgaaa aaaattttga taatttggcg aatatttgtg aattgaaaca taactatttc
      781 ggttttacgc gtacttgtaa aattcaaaat tttacaagaa attataaaac tttatgagct
      841 ttagatggtc aatacgcaaa cattcatcta gtttatagcc ttacattaaa acattttacc
      901 tattcaggtt aattgaacag tatctttaaa ggtggtgtag tcgaattttt taaaatttct
      961 ttattagact caaaattgtc tgaaaacacc aaatttcata agtaaacttc ttgaaaactt
     1021 ttcggctcga aaaaatgacc taaaattagt gaaaatttga aatgtggcca atttatcaag
     1081 cggctgcaaa caattttttt ttgaaattgt tgtctgattt tggatataga tgtaaggtat
     1141 ttaaaaaaaa gatggacgaa gaaaattggt aaaaaaattt gacaaatctc ttcgtccatc
     1201 gactttgaaa gaccttaatc aagtttaaaa cgcaagatag tcaatttata tacccaaaat
     1261 cagatgataa ttcaaaaaaa attgtttcca gccgctgcga caatgttaag ttaatttaag
     1321 gtcatttttc gagcagccat aacttctttt ttttaagttt caacaagttt cattataaaa
     1381 ttcggtgttt tcagacaatt ttgaatataa taaagaaata aagaaaaatt cgactacatc
     1441 acctttaaac taaatttcaa atgtaatttt tttttacttt tcaaaatgat tcgttctcgg
     1501 ttttgttgtg aacatttttt tgctaatacg cttacgaaat gttttgtgtg gcaatcattt
     1561 tgattgaagt tcccggcagc tacaacatat ttttagaact agaaaacaat agaacgcaca
     1621 gaatatccac gttcccatgt tttcaatttc tcttttcgaa atcctttaca aaatccgaac
     1681 gccgttctga ttcagccttg agacattttt caaaaaaaaa atcagtgtga gcattatagt
     1741 tttcagtttt gaatattcac attttgcact ttttgcctac tttccgtatt ctgcagtttc
     1801 cacatcacct tgttacatct ctcaggaagt ttctgaaagt gacaccacgc tcacacacga
     1861 tgaaattcat acccaccaac ctcccttctc tgcctccctc aacttcttcc acggaattct
     1921 atctcctctt atccagttag ggacacatat cgagtttcac caaaaataat gagaagtgtg
     1981 gtcagagaca ccacattgaa ttaactatta gttcaaaagt tgcaggtttc ctagtgcaaa
     2041 aagtttatgt tcccatctac caaacgactc accgccaatt gactttgcag tggcaacatt
     2101 gggaaatgcg acccatcata ttgttcctat tgtcatgtac aagctgtccc tgttttgctc
     2161 gcttaatccg tcaacccctt ccccgttcta attagagatg agtgaattac aatagagttg
     2221 aaacggaaat ctgagtgtct gtagctttcc gccgccgctt ttgttacgag atggtagacg
     2281 tccctttctt ggtgtttatt gtctcacaaa ctgtataata aagcaccgtt gtgtaaaagg
     2341 catttggtat caggaagata aaatgaataa aatgcaaatg cggaatcaaa aattgccaaa
     2401 taattttgac caataaaata tcaaaaataa tagaacagaa ataaacaaat aaaactacgg
     2461 agaaaatgtg attattccat taaacaacgt tttaaaccaa ctttattcgt cagtggggaa
     2521 ggttttggac aaatatcata gtaaaacttg ttcgttttaa ttatacataa tcaataatta
     2581 taggtgttag atccaatggt cgaaatttaa aaaaaaatca acattttaga aaatttcggc
     2641 aatttaccga aatattgact gacaatttta catatataag tgttttgaaa tagaatcccc
     2701 attgacgatg cgccacctta aacagtagaa ctgttccaat cagaaactaa ataaatacgg
     2761 ttattatata tatatatata tatatataac ttgtttttat tatgcatttc tcaaaactca
     2821 ccaaactcaa tctctaattt acggttgttg aggaaaatat atttatcaca tttagttgag
     2881 actctaaaag aagtacaact tttttcttgc aattcctcca ccaatccaat ttttcaagag
     2941 tcaattgctt ggagtcctcg ttttctcgta atccgatatt ccccctattt ctgtgatttt
     3001 attcagtttc gtcctcttac accaaaaatc ttcatgactt ttttggtttt ctgcttaatc
     3061 ttttttttgt ttttaaagtc taccatccgg caattttttt tgcaaatggt tgctcagaat
     3121 ttgacctcag attaaaattt tcaggaactt cctggtccca tttcgtcaca tttaataaca
     3181 aaccaaatcc ttcagcttga gtcgtccaag ccaacgcaac cctaattcga ttccacctcc
     3241 aatttacctt tttgccgttt tcgatcaaat tgtgggggct ctttttattt gaaagtcgta
     3301 aaatggcgcg tccttgtcga aaattgagat agctaatggt tccaaataaa atcaaatgca
     3361 tgaacctagt aggaaaaatc gttttttttt tcatcttcca aagaaaaagt ttaagataaa
     3421 cccaccactc cagcaatcca cctacgcgtt tttcaccgga aactgtcatt tgtttttgat
     3481 gtatgacagt gccagcactg aactaaattc ccatttgttt tgttgtggtg ttatctaacc
     3541 acgtggctct ttctctaaat tttgcgtagg ctgcaagatc cgcaatccca ccaaaaaatg
     3601 gtttctaatg tacttcatac atatcccaca tgactcagtg ctccgaaacg cccagcaacc
     3661 cccgcgtttg tctttacaaa aatgaggcgg aaatgggaaa ttttcctgct attgctatgg
     3721 taaaatactt aagctgtatt gagatttggg aaattgaaag ataagatgaa aaatccactc
     3781 tgtataatta aaaaggttta ttttttttgt aatttttgta ccgcaaattt tttcattaac
     3841 tgatttttct ttattcattc gatggcccag tttcagatct tcattatcat gtagtttatt
     3901 cattcaaact atttgtgaca tcatgatggt tgtttaaaaa taatttcgaa tgaagaaagc
     3961 ttagacctat tataattttc ttttctataa aaaatattga ttttgtaaag cgtttggcag
     4021 tgacagtacc aaaaaatgtt ttaaaatgat ttaagaaaat tttttatttt atttgtataa
     4081 tacgcgtaaa atgagtatac tcgtcatttg tcacgaatcg agttctcagt ttgctgtgaa
     4141 tatcttgtaa tagaaatcga tctcatagtg tcacttttga ggcataactt ttcaggttgt
     4201 tacaagttca ttaacttgtc aagactgggt catgttttaa agtgtaactg ttacccatgt
     4261 accctaaact ttgagacaaa ctcctcaaaa cttgtaacca gaatcctatg tagatgactc
     4321 acaaaaccct gttttcaatt tgaacgaatt tcgaaactgg ccaaaatgtt cccctcttgc
     4381 tcacctaatg cttttaccat ttaaaaagga tggtaacaac actctttcga aaatgtaggc
     4441 ggtccatttt cggtttctca taaccgtctg gctctatctc ctagtgcgcg aggagagggc
     4501 gggcggagta ggaatcgggg gcagcgcact catacactct gcccttctct tttgcacaca
     4561 cagaaaaaga tagatacaga ccaacactta ttgttgtcca gtaagcttcc agaaaactat
     4621 tttcagaatt acaaactaca tttttctaga acagtcaatt gattttttac aagattcgag
     4681 tattctaaca ttagtgaaag tatttagcca ctgcaatttt caactgtagg tttcaacttc
     4741 caaaaaaaat tcctggcatt tctaattgga tgaaaaaagg ctatctagac cctccattat
     4801 ttactcataa tttcatctcg tcctcatttt taatcccctc attgatccca tcacaatccg
     4861 ccaaaatgtt ttttaaacac ttttttcttc accagccgta tgtggtcaac tgcctaaaaa
     4921 tttcacagct atttgtcatt ccttgcagaa gaacttcatt cttctttttc gaaaatcccg
     4981 ggataacttc gtatagcata cattatacga agttatatgg tctccaaggg agaggagctc
     5041 ttcaccggag tcgtcccaat cctcgtcgag ctcgacggag acgtcaacgg acacaagttc
     5101 tccgtccgtg gagagggaga gggagacgcc accaacggaa agctcaccct caagttcatc
     5161 tgcaccaccg gaaagctccc agtcccagta agtttaaaca tatatatact aactaaccct
     5221 gattatttaa attttcagtg gccaaccctc gtcaccaccc tctcccacgg agtccagtgc
     5281 ttcgcccgtt acccagacca catgaagcag cacgacttct tcaagtccgc catgccagag
     5341 ggatacgtcc aggagcgtac catcttcttc aaggacgacg gaacctacaa gagtaagttt
     5401 aaacatgatt ttactaacta actaatctga tttaaatttt cagcccgtgc cgaggtcaag
     5461 ttcgagggag acaccctcgt caaccgtatc gagctcaagg gagtcgactt caaggaggac
     5521 ggaaacatcc tcggacacaa gctcgagtac aacttcaact cccacaacat ctacatcatg
     5581 gccgtcaagc agaagaacgg aatcaaggta agtttaaaca tgattttact aactaactaa
     5641 tctgatttaa attttcaggt caacttcaag atccgtcaca acgtcgagga cggatccgtc
     5701 cagctcgccg accactacca gcagaacacc ccaatcggag acggaccagt cctcctccca
     5761 gactcccact acctctccac ccagtccgtc ctctccaagg acccaaacga gaagcgtgac
     5821 cacatggtcc tcctcgagtt ccgtaccgcc gccggaatca ccctcggaat ggacgagctc
     5881 tacaagtgat aaatgcaaaa tcctttcaag cattcccttc ttctctatca ctcttctttc
     5941 tttttgtcaa aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac
     6001 tttttatagt cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc
     6061 tgtctgatgt actttcacaa tctctcttca attccatttt gaagtgcttt aaacccgaaa
     6121 ggttgagaaa aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa
     6181 aagaggaaac tttattgtgc cgccaagaaa aaagtctcaa taacttcgta tagcatacat
     6241 tatacgaagt tatgctagcg atccctatgg ctgagctcat ctcctccgct acccgttccc
     6301 tcttcgctgc tggaggaatc aacccatggc cgaacccata ccaccacgag gacatgggat
     6361 gcggaggaat gaccccaacc ggagagtgct tctccaccga gtggtggtgc gacccatcct
     6421 acggactctc cgacgctgga tacggatact gcttcgtcga ggctaccgga ggtaagttta
     6481 aacttggact tactaactaa ccatacatat ttaaattttc aggatacctc gtcgtcggag
     6541 tcgagaagaa gcaggcttgg ctccactccc gtggaacccc aggagagaag atcggagctc
     6601 aggtctgcca gtggatcgct ttctccatcg ctatcgctct cctcaccttc tacggattct
     6661 ccgcttggaa ggctacctgc ggatgggagg aggtctacgt ctgctgcgtc gaggtcctct
     6721 tcgtcaccct cgagatcttc aaggagttct cctccccagc taccgtctac ctctccaccg
     6781 gaaaccacgc ttactgcctc cgttacttcg agtggctcct ctcctgccca gtcatcctca
     6841 tccgtctctc caacctctcc ggactcaagg taagtttaaa cagttcggta ctaactaacc
     6901 ctgattattt aaattttcag aacgactact ccaagcgtac catgggactc atcgtctcct
     6961 gcgtcggaat gatcgtcttc ggaatggctg ctggactcgc taccgactgg ctcaagtggc
     7021 tcctctacat cgtctcctgc atctacggag gatacatgta cttccaggct gctaagtgct
     7081 acgtcgaggc taaccactcc gtcccaaagg gacactgccg tatggtcgtc aagctcatgg
     7141 cttacgctta cttcgcttcc tggggttcct acccaatcct ctgggctgtc ggaccaggta
     7201 agtttaaaca tatatatact aactaacgga ttatatttaa attttcagag ggactcctca
     7261 agctctcccc atacgctaac tccatcggac actccatctg cgacatcatc gctaaggagt
     7321 tctggacctt cctcgctcac cacctccgta tcaagatcca cgagcacatc ctcatccacg
     7381 gagacatccg taagaccacc aagatggaga tcggaggaga ggaggtcgag gtcgaggagt
     7441 tcgtcgagga ggaggacgag gacaccgtcg cggtaccggg aggaggaacc atggtctcaa
     7501 agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag gtgcatatgg
     7561 agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg
     7621 agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta actaaccctg
     7681 attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg gacatcctct
     7741 ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat ataccagatt
     7801 atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat tttgaagacg
     7861 gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg tactaactaa
     7921 ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc
     7981 gaggaacgaa tttcccctcg gatggatctg ttatgcagaa gaagactatg ggatgggaag
     8041 cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc
     8101 ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa
     8161 ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc
     8221 agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt
     8281 acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg
     8341 aattgtataa gttctgctac gagaacgagg tctaagaatt ccaactgagc gccggtcgct
     8401 accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt
     8461 aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc
     8521 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     8581 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     8641 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     8701 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     8761 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     8821 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     8881 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     8941 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     9001 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     9061 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     9121 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     9181 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
     9241 cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     9361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     9421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     9481 agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa
     9541 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     9601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9961 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10021 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10081 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    10141 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    10201 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    10261 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    10321 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    10381 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10441 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10501 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10561 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    11101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    11161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    11221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    11281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    11341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11401 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    11461 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    11521 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    11581 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    11641 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    11701 t
//
