LOCUS       sequence-37529-e        1413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Nie D, Di Nardo A, Han JM, Baharanyi H, Kramvis I, Huynh T, Dabora 
            S, Codeluppi S, Pandolfi PP, Pasquale EB, Sahin M
  TITLE     Tsc2-Rheb signaling regulates EphA-mediated axon guidance.
  JOURNAL   Nat Neurosci. 2010 Feb;13(2):163-72. Epub 2010 Jan 10.
  PUBMED    20062052
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci."; date: "2010-02"; volume: "13(2)"; pages: "163-72. Epub 
            2010 Jan 10"
COMMENT     Sequence Label: pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W
FEATURES             Location/Qualifiers
     source          1..1413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        146..350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     301..321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     347..371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1151..1168)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1379..1398
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 acatcaantg tatcatatgc caagtacgcc ccctatngac gtcaatgacg gtaaatgacc
       61 cgcctggcat aatgcccagt acatgacctt atgggacttt cntacttggc agtacatcta
      121 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      181 atagcggttt gactcacggg gatttccaag tctccacccc cattgacgtc aatgggagtt
      241 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      301 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      361 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggc
      421 ggccgcacca tgcctcagtc caagtcccgg aagatcgcca tcctgggcta tcggcatgtg
      481 ggaaagtcct cattgacaat tcagtttgtt gaaggtcaat ttgttgattc ctacgatcca
      541 accatagaaa acacattcac caagctgatc acagtaaatg ggcaagagta tcatcttcag
      601 cttgtagaca cagcagggca ggatgaatat tccatttttc ctcagacata ctccatagat
      661 attaatggtt atattcttgt gtattctgtt acatcaatca aaagctttga agtaattaaa
      721 gttatccacg gcaagctgtt ggacatggtg gggaaagtgc agataccgat tatgttagtc
      781 ggaaataaga aggacctgca tatggaaagg gtgatcagtt atgaagaagg aaaggctttg
      841 gcagaatctt ggaatgcagc ttttttggaa tcttctgcaa aagaaaatca gactgctgtg
      901 gatgttttta gaaggataat tttggaagca gaaaagattg acggagcggc ttcacaaggg
      961 aagtcttcgt gctcggtgat gtgaaaggat ccctcccccc cccctaacgt tactggccga
     1021 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     1081 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     1141 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     1201 cctctggaag cttctttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     1261 cccccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtttag atacacctgc
     1321 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     1381 gctctcctca agcgtattca acaaggggct gaa
//
