LOCUS       sequence-375292-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pQE30-His-T7RNAP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(59..73,74..79,80..96,97..103)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             146..157
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             176..193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcacc tcgagaaatc
       61 ataaaaaatt tatttgcttt gtgagcggat aacaattata atagattcaa ttgtgagcgg
      121 ataacaattt cacacagaat tcattaaaga ggagaaatta actatgagag gatcgcatca
      181 ccatcaccat cacggatcca tgaacacgat taacatcgct aagaacgact tctctgacat
      241 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg
      301 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat
      361 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat
      421 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc
      481 taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt
      541 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt
      601 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat
      661 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt
      721 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg
      781 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt
      841 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc
      901 tggcgtagta ggtca
//
