LOCUS       sequence-375293-        8854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural 
            expression of GtACR2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH4329
FEATURES             Location/Qualifiers
     source          1..8854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    94..115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(130..135,136..153,154..160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     173..195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     192..208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     192..208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6631..6653)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6753..6772
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6850..6868)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6936..7040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7041..7109,7110..7901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7259..7278)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8072..8660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8561..8580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8814..8831
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      181 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg
      241 atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact
      301 cacaacgatg gatacgctaa caacttggaa atgaaataag cttgcatgcc tgcaggtcga
      361 cgcttgcatg cctgcagcgt ttccgatacc cccttatatc agcacacatt cagtcatcat
      421 accacaaaat gttgtgatag tgataggcaa aactaccgta tttcctctat taatcttgca
      481 tgcaagacta attttcgatt aacccgtagg ggtgcaagac taatagagac tgcaagacta
      541 ttagaggctg aaatactaat tttcgtatgc tcaataattt tggaaattgg cctatttttt
      601 gtagaaactt gataccgttt aaacaaggaa aaatacacac tttttaatat ttcatcaata
      661 atttgaacga ttttgtgatt ttaagttcaa tttgcccaaa aaaagacaat ttttctgacg
      721 ttaccgcaaa gtaatgctgg tcaggcaaaa agagcggtgc aaaaatataa gagactgcaa
      781 tactaataga ggaaatacgg taattgaatt ttagtgaaac ttgcgacagt tttcctcatt
      841 tttttgttat tccggcatac gagtgtggca tacgagtgag gtcatctttt gtttcttccg
      901 tttcttcatc gcttttgaaa aaaaatgttg aagaaaactt cgtagccgat agccggataa
      961 tgtttggcac ggcgttccaa catatatcat tggaaatttt ttaagatttt tgcgaaaaat
     1021 cacattcttc acgatgagaa cacgttattg aaggaatata aatcaagaat aacatatagt
     1081 tatatttctc tattacttta acgttaaata tgagcaaatt tgagcatttt gattgcgatg
     1141 aaaagcagaa tcgagtcaac tgaaatccgt tcaaaaacct aagctcggtt cgcccgaaag
     1201 tcgatttttc caatagccga acagcactgc ttctttattt tccaggtatt tttggggtgt
     1261 aagaattata tcttctttgt ttgggtattt acgtttccga attccctttc ttgatctgtc
     1321 gctatttttc agaaaccaga acagtttatc ctttttcatg agaacctgaa tagctcaaaa
     1381 acccgtttaa tttcttttca tcatctcatt tgaaactttc ctagtcttct taatgatttc
     1441 caggctcctc ctacatttgt atctcaagat gcatacactt tatttccgct ccaattcgaa
     1501 tcatctactt ctcttttttc ttcaactttc tacttttttc cattcttttc tttcaaatgt
     1561 tcgacgtctt caagtgactg ctcttcattc tcctcatttt tctcgaattt cattcttgtc
     1621 tcttttttgc tcaaaaatgg aaaatgaaag tgacgtgaga tttggacggc gggacacggg
     1681 ggcagtagaa gcagcaaaaa ggagagaaag aggacacaaa taagaagaac gaattcaaaa
     1741 ataagcggag aggagctatt tccgtcaatt ctacctcccc aatcttcatc aattcggctc
     1801 aattgaatga cgtcacaggg agataaaccg tttggatgag cgccgtccat cactgacgcc
     1861 atcccgtttg ggacaagaaa agagaaaaaa gagcacaaag tttttggtga cggatcttgt
     1921 caatcatatg aaagttgttc tgattgattg tcagtttttt tcctactttt ttggattcta
     1981 tccacttctg aacttttgac aagtttcaaa ctttctgaat cattttctat gcattttcct
     2041 ggaattcttt ttatgtaaaa tatgaaatag aatgtttttg aattcaagtt ctgctttttt
     2101 cttcttttct gttctgttcg ggcttgggta tgcttttttt aaaaattatt ttgcacatcg
     2161 accaataagt gcgcaactta taaaattaat ttatttttgt taatttttga aatacttgta
     2221 ttgctttaag tgatctgacc tcgccctgag ctttccacgt agttatcaaa tacaaatcct
     2281 ccaagggtaa cgtacctata ttactgatct ttataataac tttatcacct gtccagttcc
     2341 agaggaattc tgttaagctt ataggcacag aaggagtcat ttctgctggt ttttaatgat
     2401 ccaaatcttt atttcaagta aaaaactgaa cacttgcgaa taaaactatc agattaacca
     2461 ttcaccaaaa atgtgtttga atctaaaact ttctcagtat tccaaatata gaaataaata
     2521 accacgacat tgctaaaatc tgtctgaatt gtgtactcct taccgtgaag taataatgga
     2581 tataatgaat cgtttgaaat gaatgatcag cacatttttg gtgaaagatc acaaataagg
     2641 aataagcgtc ggaaaataaa acgattattt cggatcaaaa atttgttgaa gatcatatac
     2701 actcgagacc aagattattc tagacaattt tcaaattggc ttctttgttt gcaaatcttc
     2761 ataataatct gtgaagtttg gaaatttgaa tttttaatct tttttcatag attatagttt
     2821 ttatttcttt gcaaaactat attaaaaacc gatgcattgt tttagggaaa ttaatgagcc
     2881 ttttgttcaa cactaaaaac aataaaatta aaattttggc ttcatcattt gacctttttt
     2941 aagttcgaaa acttttctcg tatttctgaa ccgccaattt tttcacacat ctctagactt
     3001 ttggtgcccg ttccagaaag ttaagtaatt gctattctaa gaaagttctc aacattgttt
     3061 tttagttctg attgaattct gatgttccag gaatatattt taaatttaat attctttgca
     3121 ctatttctat actaactaaa taataaatag tctaagtatg ttcaatgaga acaaaaaagc
     3181 tctatctata tttttctatc ctatttcatt tatatccttt tcattttgaa ctcaccagct
     3241 tctcttcttc ttcttgtcca tatacttctt aaaagtctcg aacttctctc tctcactctc
     3301 catcaatttc tttatggcta ccgcttgcgc tctgccgctt ccgaagaaag aaggcgttgg
     3361 cagagctctc aattctattt tttctcgccg taggctatcg atttctcagc ttctcttctc
     3421 tcttcctcac tccaccacca ccacttcggg cttcttcttt tcttcatttt cgtcttcttc
     3481 ttcatcctct tctttttttt tcagttcccc ctcactcctc cccttatatg cgcgtgcgag
     3541 aggggtgcaa aagcagcgcg catccgagaa ttgaaacgag aaaacggaga cgcagcagca
     3601 gttcgtcctc agaaaaatag ccagtaaaaa gagaaaaaga tagagagaga cctatcgcat
     3661 tttattttca aattgcaatt cctgcaattc atgtgtgcgt gtgcccattt tcaattcttc
     3721 ccgttatttt tcagaccatt accaacgttc tttctagatc ttgagacaat cttccttctg
     3781 ctcaatcgtt cgtcgtgaag acggcatcga cgacgacgac gatcagcagt aaaggatcga
     3841 tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
     3901 acattttcag gaggaccctt ggctagcatg gcctcccagg tcgtgtacgg cgagtgggcc
     3961 agcacccaca cagagtgcta caacatgagc aggatcgaca gcaccttcgt gagcctgctg
     4021 cagctggtgt gggccgtggt gagcggatgc cagaccatct tcatgatcag cagggccccc
     4081 aaggtgccct gggagagcgt gtacctgccc ttcgtggagt ccatcacata cgccctggcc
     4141 agcaccggca atggcacact gcagatgagg gacggcaggt tctttccctg gtccaggatg
     4201 gccagctggc tgtgcacatg ccccattatg ctgggccaga tctccaacat ggctctggtg
     4261 aagtacaaga gcatccctct gaaccccatc gcccaggccg ccagcattat cagggtggtg
     4321 atgggcatta ccgctaccat ctcccccgcc gagtacatga agtggctgtt cttcttcttc
     4381 ggcgctacct gcctggtctt cgaatacagc gtggtgttca ccatcttcca ggtgggactg
     4441 tacggcttcg agtccgtggg cacccctctg gcccagaagg tggtggtgag gatcaagatg
     4501 ctgaggctga tcttcttcat cgcctggacc atgttcccca tcgtgtggct gatttccccc
     4561 accggcgtgt gcgtgatcca cgagaacgtg agcgccatcc tgtatctgct cgccgacggc
     4621 ctgtgcaaga acacctacgg cgtgatcctg tggtccaccg cctggggagt gctcgaggga
     4681 aagtgggacc cggcttgcct ccctggccag gagaagcctg aggccgacga ccccttcgga
     4741 ctgaaccacg agaagaatgc cccccccaac gacgaggtca acatcagaat gttcggcagg
     4801 gcggccgccg cggtaccggg aggaggaacc atggtctcaa agggtgaaga agataacatg
     4861 gcaattatta aagagtttat gcgtttcaag gtgcatatgg agggatctgt caatgggcat
     4921 gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg agggaacaca aaccgcaaaa
     4981 ctaaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggta
     5041 actaaaggcg gaccattacc attcgcctgg gacatcctct ctccacagtt catgtatgga
     5101 agtaaagctt atgttaaaca tccggcagat ataccagatt atttgaaact ttcattcccg
     5161 gagggtttta agtgggaacg cgtaatgaat tttgaagacg gaggagttgt tacagtgacg
     5221 caagactcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     5281 agcctccaag atggagaatt tatttataaa gtcaaacttc gaggaacgaa tttcccctcg
     5341 gatggatctg ttatgcagaa gaagactatg ggatgggaag cttcaagtga aagaatgtac
     5401 cctgaagacg gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa agatggagga
     5461 cattacgatg ctgaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
     5521 tttcaggtga agacaactta caaagccaaa aaaccagttc agctgccagg agcgtacaat
     5581 gttaatatta aactggatat cacctcccac aacgaggatt acactatcgt tgagcaatat
     5641 gaaagagctg aagggcggca ctcgacaggt ggcatggatg aattgtataa gttctgctac
     5701 gagaacgagg tctaagaatt ccaactgagc gccggtcgct accattacca acttgtctgg
     5761 tgtcaaaaat aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta
     5821 acgagtaata tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt
     5881 actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt
     5941 atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat
     6001 gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc
     6061 tccctccccc cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt
     6121 cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa
     6181 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     6241 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     6301 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc
     6361 cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat
     6421 gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt
     6481 agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa
     6541 tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg
     6601 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6661 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6721 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6781 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     6841 gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6901 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6961 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7081 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7141 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7201 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7261 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7321 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7381 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7441 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7501 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7561 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7621 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7681 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7741 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7801 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7861 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7921 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7981 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8041 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8101 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8221 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8281 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8341 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8401 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8461 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8521 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8581 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8641 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8701 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8761 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8821 cagtgagcga ggaagcggaa gagcgcccaa tacg
//
