LOCUS       sequence-375315-        6774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_TNIK
FEATURES             Location/Qualifiers
     source          1..6774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1389..1412
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1419..1443
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3061..3085)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3097..3117)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3102..3431
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3282..3417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3441..4040
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3441..3460)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3937..3957
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4261..4730
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4860..4877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5031..5619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5111..5130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5779..6570,6571..6639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6402..6421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6640..6744)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
     1381 aattcatgga ctacaaagac gatgacgaca agcaattgac aagtttgtac aaaaaagttg
     1441 gcaccatgaa gaaggtgact gattactcct cctccagtga ggagtcagaa agtagcgagg
     1501 aagaggagga agatggagag agcgagaccc atgatgggac agtggctgtc agcgacatac
     1561 ccagactgat accaacagga gctccaggca gcaacgagca gtacaatgtg ggaatggtgg
     1621 ggacgcatgg gctggagacc tctcatgcgg acagtttcag tggcagtatt tcaagagaag
     1681 gaaccttgat gattagagag acgtctggag agaagaagcg atctggccac agtgacagca
     1741 atggctttgc tggccacatc aacctccctg acctggtgca gcagagccat tctccagctg
     1801 gaaccccgac tgagggactg gggcgcgtct caacccattc ccaggagatg gactctggga
     1861 ctgaatatgg catggggagc agcaccaaag cctccttcac cccctttgtg gaccccagag
     1921 tataccagac gtctcccact gatgaagatg aagaggatga ggaatcatca gccacagctc
     1981 tgtttactag cgaacttctt aggcaagaac aggccaaact caatgaagca agaaagattt
     2041 cggtggtaaa tgtaaaccca accaacattc ggcctcatag cgacacacca gaaatcagaa
     2101 aatacaagaa acgattcaac tcagaaatac tttgtgcagc tctgtggggt gtaaaccttc
     2161 tggtggggac tgaaaatggc ctgatgcttt tggaccgaag tgggcaaggc aaagtctata
     2221 atctgatcaa ccggaggcga tttcagcaga tggatgtgct agagggactg aatgtccttg
     2281 tgacaatttc aggaaagaag aataagctac gagtttacta tctttcatgg ttaagaaaca
     2341 gaatactaca taatgaccca gaagtagaaa agaaacaagg ctggatcact gttggggact
     2401 tggaaggctg tatacattat aaagttgtta aatatgaaag gatcaaattt ttggtgattg
     2461 ccttaaagaa tgctgtggaa atatatgctt gggctcctaa accgtatcat aaattcatgg
     2521 catttaagtc ttttgcagat ctccagcaca agcctctgct agttgatctc acggtagaag
     2581 aaggtcaaag attaaaggtt atttttggtt cacacactgg tttccatgta attgatgttg
     2641 attcaggaaa ctcttatgat atctacatac catctcatat tcagggcaat atcactcctc
     2701 atgctattgt catcttgcct aaaacagatg gaatggaaat gcttgtttgc tatgaggatg
     2761 agggggtgta tgtaaacacc tatggccgga taactaagga tgtggtgctc caatggggag
     2821 aaatgcccac gtctgtggcc tacattcatt ccaatcagat aatgggctgg ggcgagaaag
     2881 ctattgagat ccggtcagtg gaaacaggac atttggatgg agtatttatg cataagcgag
     2941 ctcaaaggtt aaagtttcta tgtgaaagaa atgataaggt attttttgca tccgtgcgat
     3001 ctggaggaag tagccaagtg tttttcatga ccctcaacag aaattccatg atgaactggt
     3061 acccaacttt cttgtacaaa gtggtctcga ccctgtggaa tgtgtgtcag ttagggtgtg
     3121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3181 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3361 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3421 gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3481 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     3541 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     3601 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     3661 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     3721 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     3781 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     3841 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     3901 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     3961 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     4021 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     4081 cacgacccca tgcatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     4141 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     4201 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     4261 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     4321 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     4381 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     4441 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     4501 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     4561 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     4621 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     4681 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
     4741 gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat
     4801 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4861 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5461 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5521 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5581 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5641 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5701 tcaaaaagga tcttcaccta gatccttttg cggccgcaaa tcaatctaaa gtatatatga
     5761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cagc
//
