LOCUS       sequence-375319-       10915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATP measurement, mitochondria outer membrane, Tol2 transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Yaginuma H,  Okada Y
  TITLE     Live cell imaging of metabolic heterogeneity by quantitative 
            fluorescent ATP indicator protein, QUEEN-37C
  JOURNAL   bioRxiv 2021.10.08.463131
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.08.463131"
COMMENT     Sequence Label: pT2MPEF-TOMM-QUE37C
FEATURES             Location/Qualifiers
     source          1..10915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    831..879
                     /note="synthetic polyadenylation signal"
     promoter        914..2096
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1145..2087
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2044..2064
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2112..2121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2577..2586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2943..3194
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3131..3152
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3258..3279)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3519..3538)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    3782..4006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4053..4076)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4450..4469
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4534..5133
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4534..4553)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5030..5050
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5220..5444
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5472..5488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5496..5512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5520..5526,5527..5544,5545..5550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5565..5586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(6902..7039,7040..7051)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7070..7088)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7109..7125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7133..7149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7157..7163,7164..7181,7182..7187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7202..7223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7340..7357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7511..8099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7591..7610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8270..9061,9062..9130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8893..8912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9131..9235)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9261..9716)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9398..9419)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9610..9629
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9843..9865
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9857..9874
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9858..9874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9881..9900
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9881..9899
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9913..9924,9925..10112)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gacatggcgg ggttgggggc cccgggaaaa cccccaaagc aaaggggtta agacaagctt
       61 tagggtaaga aggtagggaa ggaagaaaaa gaagaaagta aaaaccaaca tggagagggg
      121 gaaaaaaaag gcaaaagaaa tgctgggtcc cggtgccgaa gccggaaggt tggtgggggc
      181 tgggaggcag gcggaagggg gggagggctt gcctgggggg gtaaaggtgg ggcaggaagg
      241 cccggctctt taagagaswr ggacatgcag gtgttgaggg ctctgggaca ctctccaagt
      301 cacagcgttc agaacagcct taaggataag aagataggat agaaggacaa agagcaagtt
      361 aaaacccagc atggagagga gcacaaaaag gccacagaca ctgctggtcc ctgtgtctga
      421 gcctgcatgt ttgatggtgt ctggatgcaa gcagaagggg tggaagagct tgcctggaga
      481 gatacagctg ggtcagtagg actgggacag gcagctggag aattgccatg tagatgttca
      541 tacaatcgtc aaatcatgaa ggctggaaaa gccctccaag atccccaaga ccaaccccaa
      601 cccacccacc gtgcccactg gccatgtccc tcagtgccac atccccacag ttcttcatca
      661 cctccaggga cggtgacccc cccacctccg tgggcagctg tgccactgca gcaccgctct
      721 ttggagaagg taaatcttgc taaatccagc ccgaccctcc cctggcacaa cgtaaggcca
      781 ttatctctca tccaactcca ggacggagtc agtgaggatg gggctctagg aataaaatat
      841 ctttattttc attacatctg tgtgttggtt ttttgtgtgg tcgacattga ttattgacta
      901 gatcatcgcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
      961 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     1021 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     1081 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     1141 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     1201 cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc
     1261 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     1321 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1381 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1441 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1501 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1561 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1621 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1681 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1741 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1801 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1861 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1921 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1981 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     2041 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     2101 ttaaagctag cgccaccatg gtgggtcgga acagcgccat cgccgccggt gtatgcgggg
     2161 cccttttcat tgggtactgc atctacttcg accgcaaaag acgaagtgac cccaacttca
     2221 agaacaggct tcgagaacga agaaagaaac agaagcttgc caaggagaga gctgggcttt
     2281 ccaagttacc tgaccttaaa gatgctgaag ctgttcagaa gttcttcctt gaagaaatac
     2341 agcttggtga agagttacta gctcaaggtg aatatgagaa gggcgtagac catctgacaa
     2401 atgcaattgc tgtgtgtgga cagccacagc agttactgca ggtcttacag caaactcttc
     2461 caccaccagt gttccagatg cttctgacta agctcccaac aattagtcag agaattgtaa
     2521 gtgctcagag cttggctgaa gatgatgtgg aaggcggtag cggggatcca ccggtcgcca
     2581 ccatggtgaa aactgtgaaa gtgaatataa caacccctga tgggccagtc tacgacgctg
     2641 atatcgagat ggtgtccgtg cgggccgaga gtggtgatct cggcatcctc cccggtcaca
     2701 ttcccacaaa ggccccactg aagatcggag ctgtgcggct gaagaaggac ggccaaacag
     2761 aggatgtcgc agtctcaggc ggcactgttg aagtgcggcc tgaccacgtt accattaatg
     2821 ctcaagccgc tgaacgggcg gaggacattg acgtcctccg cgccaaagcg gcgaaagagc
     2881 gggcggagcg ccgcctgcaa agccagaccc gttacaacag ccacaacgtc tatatcatgg
     2941 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     3001 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3061 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     3121 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     3181 acgagctgta caagggtggc accggaggga gcatggtgag caagggcgag gagctgttca
     3241 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3301 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3361 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3421 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3481 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3541 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3601 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacctg ggtgacatcg
     3661 acttcaaacg ggccgaactg gcgttaaaac gcgccatgaa ccgtttgagc gttgcggaag
     3721 ataagtgagc ggccgctcga taagcttgat cgatgatcta gagctcgctg atcagcctcg
     3781 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3961 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4021 agaacctgca gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     4081 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     4141 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     4201 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     4261 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     4321 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     4381 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4441 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4501 catctccggg cctttcgacc tgtggccaca accatgaccg agtacaagcc cacggtgcgc
     4561 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     4621 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     4681 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     4741 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     4801 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     4861 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     4921 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     4981 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     5041 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     5101 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     5161 accgaaagga gcgcacgacc ccatgcatcg atgatctaga gctcgctgat cagcctcgac
     5221 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5281 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5341 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5401 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggctgcag cccaagcttg
     5461 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     5641 atccctagag ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg
     5701 ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc
     5761 ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc
     5821 ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa
     5881 gctttaggct gaaagagaga tttagaatga cagaatcata gaacggcctg ggttgcaaag
     5941 gagcacagtg ctcatccaga tccaaccccc tgctatgtgc agggtcatca accagcagcc
     6001 caggctgccc agagccacat ccagcctggc cttgaatgcc tgcagggatg gggcatccac
     6061 agcctccttg ggcaacctgt tcagtgcgtc accaccctct gggggaaaaa ctgcctcctc
     6121 atatccaacc caaacctccc ctgtctcagt gtaaagccat tcccccttgt cctatcaagg
     6181 gggagtttgc tgtgacattg ttggtctggg gtgacacatg tttgccaatt cagtgcatca
     6241 cggagaggca gatcttgggg ataaggaagt gcaggacagc atggacgtgg gacatgcagg
     6301 tgttgagggc tctgggacac tctccaagtc acagcgttca gaacagcctt aaggataaga
     6361 agataggata gaaggacaaa gagcaagtta aaacccagca tggagaggag cacaaaaagg
     6421 ccacagacac tgctggtccc tgtgtctgag cctgcatgtt tgatggtgtc tggatgcaag
     6481 cagaaggggt ggaagagctt gcctggagag atacagctgg gtcagtagga ctgggacagg
     6541 cagctggaga attgccatgt agatgttcat acaatcgtca aatcatgaag gctggaaaag
     6601 ccctccaaga tccccaagac caaccccaac ccacccaccg tgcccactgg ccatgtccct
     6661 cagtgccaca tccccacagt tcttcatcac ctccagggac ggtgaccccc ccacctccgt
     6721 gggcagctgt gccactgcag caccgctctt tggagaaggt aaatcttgct aaatccagcc
     6781 cgaccctccc ctggcacaac gtaaggccat tatctctcat ccaactccag gacggagtca
     6841 gtgaggatgg ggctctagcc ggaccgacgc gtctcgatag ggataacagg gtaattgatc
     6901 caatactcaa gtacaatttt aatggagtac ttttttactt ttactcaagt aagattctag
     6961 ccagatactt ttacttttaa ttgagtaaaa ttttccctaa gtacttgtac tttcacttga
     7021 gtaaaatttt tgagtacttt ttacacctct ggagctccag cttttgttcc ctttagtgag
     7081 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8401 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8581 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8761 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9061 gagcaaaaac gggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9121 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9241 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     9301 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     9361 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     9421 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     9481 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     9541 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     9601 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     9661 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     9721 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9781 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9841 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
     9901 cgaattgggt accagaggtg taaagtactt gagtaatttt acttgattac tgtacttaag
     9961 tattattttt ggggattttt actttacttg agtacaatta aaaatcaata cttttacttt
    10021 tacttaatta cattttttta gaaaaaaaag tactttttac tccttacaat tttatttaca
    10081 gtcaaaaagt acttattttt tggagatcac ttgtcgagta gggataacag ggtaatgatc
    10141 ccccgggctg cagtcgcgac gcgtcctaga gggacagccc ccccccaaag cccccaggga
    10201 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
    10261 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
    10321 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
    10381 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
    10441 agaacggcct gggttgcaaa ggagcacagt gctcatccag atccaacccc ctgctatgtg
    10501 cagggtcatc aaccagcagc ccaggctgcc cagagccaca tccagcctgg ccttgaatgc
    10561 ctgcagggat ggggcatcca cagcctcctt gggcaacctg ttcagtgcgt caccaccctc
    10621 tgggggaaaa actgcctcct catatccaac ccaaacctcc cctgtctcag tgtaaagcca
    10681 ttcccccttg tcctatcaag ggggagtttg ctgtgacatt gttggtctgg ggtgacacat
    10741 gtttgccaat tcagtgcatc acggagaggc agatcttggg gataaggaag tgcaggacag
    10801 catggacgtg ggacatgcag gtgttgaggg ctctgggaca ctctccaagt cacagcgttc
    10861 agaacagcct taaggataag aagataggat agaaggacaa agagcaagtt aaaac
//
