LOCUS       sequence-375384-        4050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL15
FEATURES             Location/Qualifiers
     source          1..4050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             680..700
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2175..2274)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2291..2310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2292..2310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2315..2331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2315..2331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2433..2452)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2444..3253
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2511..2530)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3400..3988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3889..3908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgcct tgtttaactt taagaaggag cccttcacca tgtttccaga
      721 taaagataat ctccttgaag ctctgcagca gattgcagtt caatgggatt cagaaatgcc
      781 cgaaacttca aaggaccgaa tcagattcct taaaacggat ttcgaattcc tgcatatttt
      841 cctcagcttc acagatttat catatatgcc agatttcaca tacaaagttc aaggtctgtt
      901 tcaagatgct gtcgttgacc tcaaaaaagt caacaagatc catcacattg atcgcgttac
      961 ctctcggata caagaaaaga tacggatcac taagttggaa atccgagata tccatttagt
     1021 attatccaat aaagacggta tcgatacccc tgaatttgta gtggaattca ttgatactgt
     1081 cattcagaat ctggctgatt ttgttgaact gggagattct gtcttaaagg agttgaagtt
     1141 actgaggaat tttgtttgct ttgtttcggg cagatccatg gagccgaaaa gcttacatgc
     1201 tttcgtgact catgttttac ttgtggttgg tcgtgcagca actattgtct ggttgtatta
     1261 cagtgaaaat caagaaatga atagtttgtt atctgatctt gtgcagatga agattaaact
     1321 catccagcca gacgtgcgta agatctatat tgatgtcctg caagctgtaa cgcatccgat
     1381 tatacaaatt gagaatgcag ctgattgtgt agctggcttt atggagactc tccaacacaa
     1441 cttggaagag ctaccaacac atagtcagat ggacgttgcg ttgacagttc aaacactagt
     1501 ccttcaggag atgcttcacc tcttagttga caatttcaga tgtctgtctg tacaagttct
     1561 tgaatttcac cttcaagaca ttgacactgt ggtggttgat tctggacttt gtgtttattc
     1621 attgtatgaa gatgtggcat cggaaaaagt gactcctgat ttgcaaggta ctattgagcg
     1681 catcaagaca ttgatctacc acattatccg aaaggagttc caatctagtt tgccaagtat
     1741 tcatggaata ggctatgttg atttcctctt aagcaacctg aagaagttcc atgaccgtta
     1801 ttcaagttcg cttgctcatt tcacgaatga acttcaaatg attcaaacag aactggagag
     1861 gttgcaacct tttatcagat ctgttgcatt agagagacgc aacaaatatg acaagcttca
     1921 gcattctgtg gccctggtga ttggcaaagc atatgaggtt gaatatatcg tggatgcttg
     1981 tgtaagcaat aaatttcctg attggtgcat catgctttgg ctcttggaac tcataaagga
     2041 gattagagca gtagcagcaa agatccaaag tcttgaggtt gactcagcgc cgtcacatgg
     2101 taccatcgat gcttaccctt ctcatactca aacgattaat gatgatattg taggctttaa
     2161 gggtgggcgc gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2221 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2281 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2341 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     2401 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2461 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2521 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     2581 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     2641 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     2701 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     2761 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     2821 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     2881 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     2941 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3001 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3061 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3121 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3181 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3241 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3301 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3361 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3421 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3481 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3541 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3601 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3661 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3721 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3781 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3841 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3901 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3961 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4021 ggccttttgc tggccttttg ctcacatgtt
//
