LOCUS       sequence-375393-       12564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL
  TITLE     Multi-color RNA imaging with CRISPR-Cas13b systems in living cells.
  JOURNAL   Cell Insight. 2022 Jun 15;1(4):100044. doi: 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug.
  PUBMED    37192858
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Insight."; date: "2022-06-15"; volume: "1(4):100044. doi"; pages: " 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS
FEATURES             Location/Qualifiers
     source          1..12564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      6861..6870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6867..6869,6870..6872,6873..7583)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6867..6869,6870..6872,6873..7583)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6912..6933)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7173..7192)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7520..7541
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7611..7658
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7677..7742
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7749..8316
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7910..7927)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8137..8156
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8336..8935
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8336..8355)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8832..8852
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8954..9542
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9007..9027)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9425..9436)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9617..9850
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9969..9987)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10055..10159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10160..10228,10229..11020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10378..10397)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11191..11779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11680..11699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11933..11950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12190..12211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12226..12231,12232..12249,12250..12256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12264..12280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12269..12291
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12288..12304
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12288..12304
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12354..12373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg aagatccccc
     3481 agatcatcga gaacaacaag cacctgttcg gcgcctacag cactatggcc ctgagcaaca
     3541 ttcgcaacat cctggatcac atcgccaccc tggctgacat cgaaaatgac ttctacagcg
     3601 atagcgactc ttattggtac cacccatgca tcaagatcat cgaccctcgg aacaactgca
     3661 ccgacgccgc caaggctgat ttcgtgatcg agaagattaa gtctcatttc ccattcgtgc
     3721 caatcgtggc cgaggcccag aggcagtccg atatcctgag agccaagaag cagctggaca
     3781 tcgccgtgag aaatagggat taccagaagc aggaggagct gaagcaccgg cagaagtccc
     3841 tgctgtccgt gaccaatgcc gatatctacc gggtgctgaa taacatgctg tgggtgctgg
     3901 ccaactacgc caaccagacc gtggcctacc tgatcagcga catcaagttc gaagatggtt
     3961 ccgactttct caattataac gagcagcctc tgtcccggat gctgaataac tacttcacca
     4021 tcgccctccg ggacaccgct aacaagtaca gctatagccc tgaggccctg agcttcatcc
     4081 agtcaagtag gtataagact gagaatcggc ggaaaatcct ggacacagac tttttcctgt
     4141 ctatccagca caacaacagc gatactaacc ctaagaacct tcacctgtcc ggcgtgggcg
     4201 tggccctgct gatttgtctg tttctggaga aaaaatatgt gaatgtgttt ctgcagaagc
     4261 tgcccatcta cggaccctac aagaagcagt ccatggaggc caacatcatc agacagagct
     4321 tttccatcca caccgtgaag ctgccaaagg agcggattgt ctctgaaaag agcgacttca
     4381 ccattgcact ggatatgctg aacgagctga agagatgccc caagcagttg tttagcaccc
     4441 tgtcctacgc tgaccagaac gccttcagaa tcgtgagcag tgatatgagc gacgtgctgc
     4501 aggtgaggca tactgaccgg ttcgcccagt taagcctgga gtacatcgat cggcgggaac
     4561 tgtttagcga cattagattt cacctgaaca tgggcaagct gcggtacctg aaaaccgctg
     4621 acaagcactg catcgatggt gtgtctaggg tgagagtgct ggagggtaaa attaacgcct
     4681 ttggccgcat tcatgagttt gaggccaggc ggaaggagca gggattcgtg gagtggcacg
     4741 agcaggcagc ccggctgatc aagaccaata agaatatcga aatcagggac tttgagcacg
     4801 tgaagcgcga cgacgccaca cccgatagct acccctacat tattgacact tacacccact
     4861 atctgctgga gaataataag atcgagatga ggtttggcga ctacctccca aaactggtga
     4921 aggtggacga ccagaagtgg accgtgtaca acgaaatgcc cacatgttgc atgtccgccc
     4981 tggagctgcc cgccatgatg ttccacatga tgctgtgcgg cagcgaagca acagagcatc
     5041 tgatcaaggc cgaggtggag aagtataaga agctctttgg cgccatggct aacggcacac
     5101 tgtctaagga gaatatcggc aacttcgaga ttgccatgga ggacctgcca aagaaagtga
     5161 tcgactgcgt gaataggaag aagtctggga agtcttttgt caagcagatt aagaaggaga
     5221 tcgccgagat gctggccgac accaacctga ggattgtgag actgaagtcc gataagcgca
     5281 gcgtggcctc cacccagaat aagatgggca aacggggctt tagaagcatt cagcccggca
     5341 agctggccga ctggctcgcc gccgatatcg tgaaacacca gcctagcctg tttagcggcg
     5401 acgactacgg taccgatagg attaccggca tgaattaccg ggtcatgcag tctactatcg
     5461 ctaccttcaa tgctgccacc cccgagcaca gcctggacga gctgaaaaga gtgtttagcg
     5521 ccgcacagct gattcaatgt caggagaggg agcacccttt tctccagaag gccctgaatc
     5581 gcaatcccca gaacaccatc gacttctacg agttctatct gcaggctaag agagcttact
     5641 atcagtccct gcggcagtct atcgagaacg gcgaggacgt gaaactgcca tacctgaacc
     5701 ctgatcgcaa taagtgggtg aggagagggg ccagctttta cagcaccatg ggagatatct
     5761 acctggagga catgcccatt gagctgccaa ggcagatgtt cgacaaggag atcaagaagg
     5821 ccctggccca gctgccttcc atgaagggcg tggatatgaa gcagagcaac gtgaccttcc
     5881 tgatcgccga gtacctcaag agagaactga aggatgactt tcagccattt tatcagtgga
     5941 acagaaatta caggtttaca gacatgatga tttgcgagga gaactgcgac accaaggccc
     6001 tggcatccaa attcatccct ctggagctcc gcgaggagat ctgggagaag cggggcgagc
     6061 tgaaagatgc ctacaaaaga tgggctttcg gcagactgaa taagaatcct gatatcaagc
     6121 ggctgagccc tgccgagaag gacgaactgc tggaaaagcg catcgccaag tgtcgcaacg
     6181 agtaccagaa gtccgagaag atcattcgga gatacaaggt gcaggatgtc ctgatgttca
     6241 tgatggtcaa gtccatgttt gctcagggag tgttcaccgc cgagtccaag gagtttgccc
     6301 tgtccacaat cacccctgac gccgagcggg gcattctgtc cgaagtggtg cccatcgatt
     6361 tcaagttttc catcgacgga aagacctaca ccatccactc caacggcatg aagatcaaga
     6421 actacggcga tttttacaag ctgatcaacg acaagagaat gaagtctctg ctgaatatcg
     6481 tgacccactc tactatcgag aaggacctgc tggagaagga gtttagcacc tacgacgata
     6541 agagacctga ggccgtggag atcatcttcg agtttgagaa ggccgcctac gagaagcacc
     6601 ccgagctgaa ggagctgctg actcccgagc gcaagtttga tttcagggcc ctgctggagg
     6661 agctccaggc caagtctgac ctgtctcaga tcgatgccag atatctgagc cagatcgcca
     6721 acgccttcaa cgccaacgcc tacccccgga atctgaagat cacatccaac atccctgaga
     6781 tcgcccagga gatggtgaac atcttcagga tcaccgaccc ctacaagaaa ggcaagaaga
     6841 gtctagaggc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     6901 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6961 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     7021 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     7081 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     7141 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     7201 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     7261 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     7321 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     7381 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     7441 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     7501 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     7561 tcggcatgga cgagctgtac aagtcgaatt ctaagccagg attccatatg aagcgacctg
     7621 ccgccacaaa gaaggctgga caggctaaga agaagaaaca acgcgtgcag ctaagcgact
     7681 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     7741 agtaaggatc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     7801 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     7861 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     7921 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     7981 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     8041 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     8101 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     8161 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     8221 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     8281 ggggacgtgg ttttcctttg aaaaacacga tgataaggat ccaccggagc ttaccatgac
     8341 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8401 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     8461 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8521 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     8581 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     8641 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8701 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8761 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8821 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8881 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     8941 atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     9001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     9061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     9361 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     9481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     9541 gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9601 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     9661 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9721 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9781 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9841 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     9901 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag
     9961 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    10021 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10081 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10141 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10201 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10261 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10321 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10381 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
    10441 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10501 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10561 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10621 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10681 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10741 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10801 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10861 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10921 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    10981 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11041 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11101 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11161 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11221 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11281 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    11341 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11401 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11461 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    11521 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    11581 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    11641 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11701 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11761 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    11821 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    11881 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    11941 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    12001 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag
    12061 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    12121 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    12181 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    12241 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    12301 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12361 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12421 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    12481 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    12541 aatatgtact acaaacttag tagt
//
