LOCUS       sequence-375394-        4413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL18
FEATURES             Location/Qualifiers
     source          1..4413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             680..700
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2538..2637)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2654..2673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2655..2673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2678..2694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2678..2694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2796..2815)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2807..3616
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3763..4351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4252..4271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgcct tgtttaactt taagaaggag cccttcacca tgcaggcaag
      721 ccattcgttt ttagagggat ttcacttctc caaggagtct cgtcctcctc acacaccgca
      781 gaggattgaa gagaaaggcc tgccaatagt atctcattta tctaaaactc tgcagcagat
      841 ggaggatgaa tgcaattcat tcttcgttga atatctgttt gcacaggaac ttgatgccct
      901 tagcgttctg caacgtattg aagaatattg gggctcattc cgtatgtatt tggaggaatt
      961 cagatcaaaa gtgtctgatt tccttgaaat ttcacaagaa ggtatgtctt tgttagatag
     1021 actgaggttc ctcaaaaggg atttcaagtt cctgagtatc attatcgaat tgcatatctt
     1081 caaagaggaa ccacatgtaa gctgggaaaa agtccgagca ttgttccacg gtgctacaga
     1141 tgaactcata cagatgtaca gtacggaagt aagtccgtgg agtcaacaat tttatgacta
     1201 tttctgccac ttgcaatatg agttgcagca gaccaagttg gaaatcatta aggctaattt
     1261 cccctttccc aaaatatcag ccaatgagga tggtattgtg attcccgatt ttgttaagaa
     1321 atttattgac agtgttgctg ataatatcag taattcactg aaatttgatg atctaagttc
     1381 accactttgt attgggggac gaagcattat ggttcaaata gaaatggttt tgaaggggtt
     1441 gaatttcctc tctagttttg tctgctttgt ttcagataga tgcatagaga ctcgggtcaa
     1501 acatgctttg ttcactcatg ttgtacaagt ggcctggcaa acaactatgg ctacgtggtt
     1561 gtatcttccg agcaatgaat acatgtatca agacacagct ccaaacggtg agaatccttt
     1621 gctttctgat cttctgaaga gcaaaatcca gcctattcag tcgagcatat gcaagttcta
     1681 ctgtcatatc ctgcaagctc taaatttagt tcagtcacaa tggtaccccg ttatcaatgc
     1741 taagtatgta ttcgattgtg aagttgggtt tctggagtct cttcgatgca ctttaaaagg
     1801 attaccagtc tctagtaact gcattgcaat aaaggcagaa cttcaggaga cgctcaactt
     1861 cttcggagat actctagtca atctaccaac acaggtcatt gaacttcatc ttcaggatat
     1921 agacttttca attgttgatg caggacttct ggttttctca ttaaatgatg ataatgagaa
     1981 tcttgatttc acgggaaaaa tccaaagtat gcaaagtgtg atctacctca tctatagaaa
     2041 gaaattcctc cttcagttca acttacctgg aattgacaga gtggactctg ctgatttcat
     2101 tttagacaac agggagaagt ttcagagcat gtattcaaat tcagttgatt cggttcaaag
     2161 ccagcttccg attattcaga aagaactcaa gttctttcaa gctgtcgtag aacagcaaga
     2221 cggacttcaa cattttgcaa agaagactac tcatttggtg tttgaggtag aacatatggt
     2281 tgatacttgt aagaaaaaag atgttcctga ctggtgtctt tttatctgga tcttgaacat
     2341 cggtgaggat attagaatgc tcatggcaga ggtagcagag attcgtaatg agttactctc
     2401 atctccaaac aagctaactt catttgtgca gttagttctc aagggattcg ttcggatctt
     2461 tggtgttgct tcttcacaat ttgctagtaa acaaaggatc aatgaagaaa tagtaggctt
     2521 taagggtggg cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     2581 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2641 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2701 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2761 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2821 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2881 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2941 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     3001 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     3061 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     3121 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     3181 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     3241 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     3301 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     3361 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     3421 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3481 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3541 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3601 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3661 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3841 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3901 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3961 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4021 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4081 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4141 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4201 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4261 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4321 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4381 cctggccttt tgctggcctt ttgctcacat gtt
//
