LOCUS       sequence-375395-       12993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL
  TITLE     Multi-color RNA imaging with CRISPR-Cas13b systems in living cells.
  JOURNAL   Cell Insight. 2022 Jun 15;1(4):100044. doi: 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug.
  PUBMED    37192858
REFERENCE   2  (bases 1 to 12993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Insight."; date: "2022-06-15"; volume: "1(4):100044. doi"; pages: " 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS
FEATURES             Location/Qualifiers
     source          1..12993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      7290..7299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7296..7298,7299..7301,7302..8012)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7296..7298,7299..7301,7302..8012)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7341..7362)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7602..7621)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7949..7970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8040..8087
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8106..8171
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8178..8745
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8339..8356)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8566..8585
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8765..9364
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8765..8784)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9261..9281
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9383..9971
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9436..9456)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9854..9865)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10046..10279
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10398..10416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10484..10588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10589..10657,10658..11449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10807..10826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11620..12208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12109..12128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12362..12379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12619..12640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12655..12660,12661..12678,12679..12685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12693..12709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12698..12720
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12717..12733
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12717..12733
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12783..12802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg gcctttgata
     3481 agcctagacc caaacggagg caggccaggt ctgattttta catcactgac aaggctatca
     3541 tggggggcta ctttaatctg gcccagctga atttctttaa gaccctgatg gagatcttca
     3601 caaaagctgg catcgatgtg agcaaaatca agcaggataa cagccctaag tacctgatga
     3661 ttttgattaa gaaactgacc catgatgatg aaaagattga tgacaagaag tgggccgatg
     3721 ccctggatct gagcaacgaa tgtctcctga agctgcagca gctgctctat aaacatttcc
     3781 ccttcctggg accagtgatg ggcggagagg cttcctacaa tatctacaag ctgaaggacg
     3841 gccaccctga agtgaaggtg gccaatgacg tcatgagagg catgaagctg gaggactgcc
     3901 tggctgtgct gaggcatttc gcactctgcc tgaatgactg tgccaacttt tatacagcct
     3961 ttaacccata taactctatt gacgcccaga aggagcagta tttgagccaa aataaggtgg
     4021 ctgtttggct ggaaaaggtg aaggatgcaa gcaggagaat cgtgaagcag aacaatcagc
     4081 tgacctctca ggacatggaa tttctgaccg gcatcgacca catgaagccc caggacaagg
     4141 tggatgactt cggcaacatc atgaccgaca agaaaggcta caccattaag gagtacgtgg
     4201 agtatgagga ttattatttt cgcatcaggg gaaaacgcta tctggtggat gcagccggcg
     4261 ccaaactggc cgatcaggaa ccacgaaatg ccctctctga cttcggcgtg gtgttcctgt
     4321 gcaccctgtt tctcgaaaat gatcagagca gaagaatgct cgacgagctg agactgttcg
     4381 agactggtcc ttacgacggc agcagggacg gagatgagtt caagaacgac atcctgaggg
     4441 agattctgct gttctacaga gcccgcatcc cccgcggaaa gaggcttgat cctatggatg
     4501 acactacact gctcgccatg gacatgctca acgagctgag aaagtgccca atgcctctgt
     4561 acgacgtgct gtctagggag ggccagcgct tcttcgagga cgaggtgaaa caccctaacg
     4621 acctcactcc cgaggttgcc aagcgcctca ggagttccga taggttccca tacctggccc
     4681 tgcgatacat tgatctgaat aagtgtttcg ataacatccg cttccaggtg cagttaggga
     4741 agttcaggta caaattttac gataagacca ctattgacgg tgagcaggtg gttagaggcc
     4801 tccagaagga agtgaacgcc tacggccggt tccaggatgt ggagcactac agacaggaaa
     4861 agtatgcaga tatgctgcag cagactgagc tggtggaaac tggcgaggag gatatcacca
     4921 ttgccaattt catccccgat actccccagt ctagccccta cctgagtgat aggaccgcta
     4981 gttacaatat tcacaacaac cgcatcggcc tcctttggaa catgcccgga gagcacgaag
     5041 ttctgaccgg cgatgagaaa atgtacctgc ctgacctgaa tgtggacgac aacggcaagg
     5101 ccgatgtgtt cctgcccgcc cctaaagcct ctctgtccgt cagggacctg cccgctcttg
     5161 tgttttatct gtatctccaa aatcagcacc ccgacctgac accagctgag gccattatcc
     5221 agcagaagta taatgccctg gtgagatttt tcgaggatgt cagcacaggc aggctcctgc
     5281 ccgtgaaagg aattgctgag ctggaggccg ccatcgacaa gtatggacac ctgaccgtgc
     5341 acgaaattcc agaaaagctg cgcgactacc tcgccggagt ggccggaact gaagatgacg
     5401 acgactgcgc cgatcgactt gactgctacg caatgggcat tctggaaaag agatatagac
     5461 aggtggccgc caggatcgac cagctgaagg aggccagaaa gatggttggc gacaaaatga
     5521 acaggtatgg caaaaagtcc tacatcgacg tgagacccag gagactggca gagcagctgg
     5581 cacagtctat cgtggagtgg cagccagcca ctgataatgg acgccacaaa atgaccggga
     5641 agaacttcca gcggctgatc gcctttctgg ctcgctatat cgacgaggtg cctctccagg
     5701 agctgaaaga cctgatgaca agggcccgcc tgctggaggg gccagtggcc catccctttc
     5761 tgcagcaagt gttggacaag aatcccctga acatcgaggt gctgtatgat ctctatttta
     5821 aagaggaaaa aagatacctg gacaagtaca tcgatattga gatcaacaaa cagggcgagg
     5881 aggtatattg gctgaaggct gacgccgatc tgtctaaaat ccccttcctg aatagctcta
     5941 ggctgaggtt ccacaaacgg gatagcgcca gcttccagcg ccttgccgcc aggtacctcc
     6001 aggtggacgg acgccagtcc actattctgc tcccagatgg acttttcacc cctcatattc
     6061 tgagtgccct gaagtccact ctgtaccagg aaaaccatga cctgcaggat caccttcagg
     6121 atcaggagct gaaccagaat gcctcttatc ttatgtccgt gtatctggag catgtgctgc
     6181 atgactctgc ccagcctttt tacctgtcaa acagcaatga ttcccccttc gcccggagat
     6241 atgatctctt cgctatcgtg cagaataaga agagaggcgc tgaactgctt ccaggccctc
     6301 tgaccccagt ggagatcaat aggcgcctga cagctaagac accagacggc tccgcaaagc
     6361 tcattgaacg tgacatcgtt gccctcctgg aggacatggc ccgcaagacc gagcagagaa
     6421 ttcacaagct gccccaggat ctctatctga aaaagaagaa gcaggcccag gaggagcgga
     6481 ttaagctgca gcgccgcctg aaacacaaga tcacagatgt gaagaagaac gagcgcgcca
     6541 ttcggagata caaaactcag gatatggcaa tcttcctgat ggcccagcag atgctcgcca
     6601 accaactgtc ttggcagaat caggaaggag aggtgcccct gaagctggcc aacgtgtgca
     6661 atgacggctt cctgagccag accatgaagg tgagctttcc ttacgaagtg ccactgaaag
     6721 aggacaagac taagaaagtg acaattgata ttgtgcagga caatatgtcc ctgaagaact
     6781 acggcctgct gtataagctg taccacgacg agcgcgtggt gacactgctg cagaacatcg
     6841 tggaccagca gcagatttct ttcgccgagc tgactgcgga gctgagcctc tatgaccaga
     6901 acaggagcaa ggccttctcc aactttcagc agtttgagaa actggccttt gaaaccaata
     6961 gggaagcact gtcaaacccc gagcatagag gattcttcct cgaaaaggtg aataagaaaa
     7021 accaggtcat caggtacgag cggaggaaca attttgatgc tctcctggac ctgctggatg
     7081 ccatcgaggc ccaccagctg gaccctcgcg atagacagtg gctgacatct gtggccaacg
     7141 ccttttgtgc caacagctat tacaagattg acatgcagca gatcgagaaa aagctgccta
     7201 ccatcatcca gcaaatcgtg gaaattgtgc agaggatcac agataaagcc agccacccca
     7261 gcaataggag tctagaggcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
     7321 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     7381 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     7441 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     7501 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     7561 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     7621 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     7681 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     7741 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     7801 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     7861 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     7921 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     7981 ggatcactct cggcatggac gagctgtaca agtcgaattc taagccagga ttccatatga
     8041 agcgacctgc cgccacaaag aaggctggac aggctaagaa gaagaaacaa cgcgtgcagc
     8101 taagcgacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
     8161 acgatgacaa gtaaggatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     8221 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     8281 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     8341 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     8401 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     8461 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     8521 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     8581 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     8641 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     8701 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataaggatc caccggagct
     8761 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8821 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8881 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8941 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     9001 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9061 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9121 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9181 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9241 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9301 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9361 ctgacgccca tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     9421 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     9481 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     9541 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     9601 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     9661 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     9721 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     9781 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     9841 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     9901 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     9961 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
    10021 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
    10081 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10141 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10201 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    10261 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
    10321 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
    10381 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
    10441 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    10501 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    10561 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    10621 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    10681 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    10741 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    10801 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
    10861 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10921 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10981 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    11041 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    11101 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    11161 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    11221 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    11281 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    11341 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    11401 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    11461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    11521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    11581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    11641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    11701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    11761 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    11821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    11881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    11941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    12001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    12061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    12121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    12181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    12241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    12301 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    12361 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    12421 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
    12481 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    12541 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
    12601 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    12661 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    12721 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
    12781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    12841 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    12901 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    12961 tatttgttaa atatgtacta caaacttagt agt
//
