LOCUS       sequence-375397-        4164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL8
FEATURES             Location/Qualifiers
     source          1..4164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             680..700
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2289..2388)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2405..2424)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2406..2424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2429..2445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2429..2445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2547..2566)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2558..3367
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2625..2644)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3514..4102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4003..4022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgcct tgtttaactt taagaaggag cccttcacca tgagtgatct
      721 tattgatgat ttattgaatg gtttgaagaa aataaagaaa gaattcattg cttcaaaatg
      781 ggatgacatt gaaaagctaa gaatggaatt gagatttcta agaacatttg tcctgtttcg
      841 gaattggaat ttggatgact tttatgataa gatgttactg aatatagaaa agctcaaaga
      901 tctaatagat acaattttca atgaagatga atttatcgta gccaaataca acatggacag
      961 tcttccgtct tacctgcgga aagaaatcaa tagttatttg agattgaaga atgtagccaa
     1021 tatcataact gaggagaata tgtttgaata tacagacagc ctcctcaaga acctccatga
     1081 tctaccaaag tattgttctg atttgcttca acccctcatg agtgaataca acattcttcg
     1141 gcaagtatgc acacatctca gagatttcta tcagttgaaa tgcaacaaaa caacaaaaga
     1201 atttctctat gcttggtacc gaatgacggt tgatagagta acacaattct gttttgatct
     1261 ttggactgga aagtacaaaa actatggata tgagtatgcc ttctctgaat gctcttccaa
     1321 gatcacttct ctactcatcg acataatccc tcttgagttg gaggttctac acatttctac
     1381 ttctaagctc attaaagagt caacatcaaa agaactagaa ggattggtta agcaaatctt
     1441 aaaagcatct ccaaggattc ttcaaaataa tctcattcat ctccaacgac gcatggaagt
     1501 tgcagtagat gtaaattacg ctccaactcg aagcattagt gtcatgatgg agttcctatt
     1561 gatctttcta actgatatgc caaagcggtt tatccatcgt gaaaaattga acgatatgtt
     1621 ggcacatgct ggaatgctta ccagaaaaat atcttttctg gtgatcaagc tgttggagga
     1681 gatctctgag gataatatca atgaagcgga cttttcagct ccagactttt tgcaagaaat
     1741 cgaacaaatg aagggagaca tcagacacat ttttttaaaa gctcccgagt catctcaact
     1801 tcgttttcca atggatgatg gtttcctctt catgaatctt ctactcagac atttaaatga
     1861 tttgctcatt gccaatgtct cttcagttac tctgataaag aaagaaatta ggatggtgaa
     1921 agaaagcctt gaattcctaa catcatcttt cggaaaagtc aggaaaacgt tggatggcac
     1981 tagtggagta gttaaagatt gttggttgca tgctttggat gtggcatatg aggcagagca
     2041 tgtcattaat tccattcttg tcagagataa aactctcttg catttagtct tctcacttcc
     2101 gaatgtcact gataagatca agcttatcgt ggcacaagtc accagtttac agctggagga
     2161 taagaatggg gatgaacccc ttgatgcaaa gtcttcccat gagtcaattg tgttaacctc
     2221 atcacctttt gttgaggtaa cagtaggtca tgaggaagaa gaagcctgga tcaagggtgg
     2281 gcgcgccgac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2341 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2401 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2461 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     2521 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     2581 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     2641 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     2701 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     2761 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     2821 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     2881 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     2941 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     3001 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     3061 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     3121 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     3181 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     3241 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     3301 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     3361 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     3421 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3481 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3541 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3601 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3661 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3901 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4021 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4081 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4141 ttgctggcct tttgctcaca tgtt
//
