LOCUS       sequence-375400-        4233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL16
FEATURES             Location/Qualifiers
     source          1..4233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             680..700
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2358..2457)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2474..2493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2475..2493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2498..2514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2498..2514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2616..2635)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2627..3436
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2694..2713)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3583..4171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4072..4091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgcct tgtttaactt taagaaggag cccttcacca tgttttccgg
      721 cgaatatttg tcttcaacaa agcatcaccc gctcaaattt ttgcagcgaa tcgaagacga
      781 atggggtagt tacatgccgg agactataaa ggaacgagtt tatttactca aaacagagtt
      841 caaattcatg gatatgtttc tcagtattca gagcttcact gataaaccaa acatgctaac
      901 tgtcactcag aaagttcacg ctctgtttca agattccgca tttgatgtat caaaaatata
      961 tcaaaatctc aatcgtctta cttccctgct gcagaacaaa attcgggtga ctaagatgga
     1021 aatcggagct aattactcct tcttctccga gatttcgttg caacaacttt cggaaaagag
     1081 tggggttgat aattccgagt ttgtaatgaa attcatcaat ggtgttgtag agaatgttag
     1141 cgagttagca gagatggatg atctctgttc tcgcgaaata caggaggttt taaaagagct
     1201 gaagttgttg aagagtcttg taggattttt atcaaacagg tggtatgcag agcctcagag
     1261 cgtacgtact ttcttcggtc atgttctatt tgtggctggt tctgcagcaa tggttgtctg
     1321 gttgtacatt ccaagctatg acaataacag agatcaggat ttggttcctg gtgagatgaa
     1381 tttcttgctt tcttatctcg tgcggatgag gattaagcct gttaatccgt gcatccgcaa
     1441 aatctatgtt gacgtactac aagctttgaa atggacaatg caatccgaat tgtctctgaa
     1501 tatccaaaat gtgtatgtag ctgagattga agctggcttc atagagattc tcatacacaa
     1561 cttggaggag atacggagta ttagtactct cagtcggata gagtttttga atcatctaat
     1621 ggcaaccctt gtggagatgc tcaaattctt gagagccagt ctgatccatt tgccaaaatt
     1681 gggtcttgaa tttcatctta aagatattga tactatcagc attgatgtgg gacttctcgt
     1741 ttactcgtta tatgatagag aggagcagga tgaggaagtg aaccaaacac tatttcgtga
     1801 tatgcccaaa agcattcagc atatcaagga agtgatcttc ctcgtgagtc gaaaggcatt
     1861 tcaatctaat ttgcctaggg tccatggact aggctgtgtt gatttccttc tgaataactt
     1921 gaaggagttc caagaccgtt attcagattc acagtattct tttgttaaga gccaacttca
     1981 agttattcag aaggaactcg agggcttaca accttttctc aaggatgtgg cggaagagtg
     2041 gtacaataag catgaaaggc tccaccattg tgcagcacta ctgaatggca aagcttatga
     2101 ggtggaatat atagttgatt cttttattaa aaaagaagtt cccgaatggt gtcttgttcg
     2161 ttggctcttc gacatcataa aggaagttat acttatcaag gaagaggtca caaagattcg
     2221 gaaaaaggaa cttttgaagt ttgactttgt attaaatgat accttggata ccacccctgc
     2281 tcatatatca tcagaatcga ttaacactcc aaggataacc gatgaagaag ttgttggttt
     2341 taagggtggg cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     2401 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2461 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2521 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2581 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2641 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2701 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2761 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2821 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2881 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2941 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     3001 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     3061 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     3121 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     3181 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     3241 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3301 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3361 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3421 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3481 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3541 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3601 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3661 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3721 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3781 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3841 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3901 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3961 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4021 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4081 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4141 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4201 cctggccttt tgctggcctt ttgctcacat gtt
//
