LOCUS       sequence-375401-       13104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL
  TITLE     Multi-color RNA imaging with CRISPR-Cas13b systems in living cells.
  JOURNAL   Cell Insight. 2022 Jun 15;1(4):100044. doi: 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug.
  PUBMED    37192858
REFERENCE   2  (bases 1 to 13104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Insight."; date: "2022-06-15"; volume: "1(4):100044. doi"; pages: " 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS
FEATURES             Location/Qualifiers
     source          1..13104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      7401..7410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7407..7409,7410..7412,7413..8123)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7407..7409,7410..7412,7413..8123)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7452..7473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7713..7732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8060..8081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8151..8198
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8217..8282
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8289..8856
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8450..8467)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8677..8696
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8876..9475
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8876..8895)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9372..9392
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9494..10082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9547..9567)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9965..9976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10157..10390
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10509..10527)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10595..10699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10700..10768,10769..11560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10918..10937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11731..12319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12220..12239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12473..12490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12730..12751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12766..12771,12772..12789,12790..12796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12804..12820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12809..12831
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12828..12844
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12828..12844
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12894..12913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg agcaagatca
     3481 tgtgcatctt cgccaagctg aacatccaga tgcctagcaa taacaattcc ctgaaccagt
     3541 tcatcaccac catggacaat aatgagacca gcattaagca gttcgtgctg ctgggcgagg
     3601 acaatagcca gtaccgggac tacgccggct cctacttcac caaggccaac ctgaacttct
     3661 ataggaccat gaaaaccgtg ttcaggagaa gcggaatcat gaaggataac aggctgggca
     3721 acgtggagtg gcgcgacgac aacatggaga cagccctgtc cagctttgat atcacaacca
     3781 tcagcgccac acagatgagt agcctgagcg accacctgtt ccgcaatctg cctatgttcg
     3841 gacctatcat gagccacgac aagaatgcct tcctgtcccc tctgaagcgg gccaaaaaga
     3901 cctttgacaa ggccaagaag gacctgaaga acgccaaaaa gacacaccag gacgaggtga
     3961 agtacgagaa gctgaagaag gaggccgaga aggagtacaa ccagcagaag gaaaacttta
     4021 aaaacgagat gtacgggact gaggccaagg acatgtggga taagctgatc aacatcgccg
     4081 gcgctctgct gtactacgcc aacctgtaca ccgccaagaa cccctacagc accgaggacg
     4141 atgagaataa gcagaaggaa cgggagatca agatcgccaa gtggatgaac agcgtctttg
     4201 agggcgaaaa gggaatcctg attgagcgca aaaagtacaa gtacgaagat accctgtttc
     4261 tgattgacca ccagaaggag accgatcgca tggagaagag aggcaacaaa tacgtcgagc
     4321 gggacaattt ctactttaag attaagatcc tggacgagaa caataagtgc ctgggcctga
     4381 ctgattttgg gcgcttcttc ttttgcagcc tgtttctgcg gcgctccgac agtgagctgt
     4441 acgccaagtc cgtgaagctg tttgagaact ccccatactg cctgactgag caggagctga
     4501 agattaagca ggccgaggaa aacaagaggg caagtaccga gagccagaag tctgaccacc
     4561 aggtgaaccc caagactatc gacaagacag agagcgccca gattaacatc atccgcgaga
     4621 tgatgcagat gcacaggatc agaattccca gagagaagaa gattgacttc acccccagcg
     4681 agtccatgct gctgatggat attctgaacg agctgcgcag atgcccacag gagctgtatg
     4741 acaccttcaa tcagacagac aagctgaagt tcgtggtgga caaccctaac gaggaccctg
     4801 agtctgacag caagatcact ctgactcgga gcaaggaccg ctttgcccac ctggccctga
     4861 ggctgatcga ccatactgat gcactgggca gtatcagatt tcatctgcgg ctgggactgt
     4921 tccgctttag gttctacgat aagaagctca ttgataatag tgagactctg cgccgcgtgc
     4981 acaaagagat caacggcttc ggcaagtggc acgtcgtgga gtccctccgg cgggagaagt
     5041 ggggaaaata caaccagaaa ttcgagctga atgacgacgg cctgctgcaa ttcgtgcccg
     5101 acagcgcaga gtccgaccca tacatcaccg actggcagac cacctacaat atccacgcca
     5161 acaggatcgg gctgttctgg aacgggaact ctcagtctga ggactactac atccctgagc
     5221 tcaagaccat ggtcgacgag aatagcaaag tgattgcccc cgtggagatg aagatcccac
     5281 tctgtagcct cagcatctac gatctgcccg ctatcctgtt ctacacttac atcttcaatc
     5341 tgtacaatgg aaaccggctg ggcctgccaa acgccgagaa aattatcaag aataagtaca
     5401 ccgccctcaa tgagttcttt gggttcgcca agaaaagcga aagcacaccc gaggagctga
     5461 tcaaaaagca ggaggagctg aacctccacg acagcgagat ccctgagcgc ctgctctatc
     5521 tgatggggaa gatcaaaacc cctaagggca agatggccga gtggaaaccc aactccaagc
     5581 gcaacaacca cgccaaggag ctgctgaaga agattatcga ggaggacgag tggaggatca
     5641 agagcttcaa ggacaagaaa aataagattg aggacaagaa ccggtatggg agaaaggact
     5701 actccgacat ccggcatggc gtgctggcaa agtacctggt ggagagcatg atgatgtggc
     5761 agcccggcaa gcagaaagcc gagacaaaga tcagcagtgc caactaccgc gctctgaggg
     5821 attatctgag taactacaac aatagctcca acaatctgca gtatgtgctt gaagctgccc
     5881 acctgattga ttccgagaac ccacaccctt ttatcgacaa agtgaagggg agtaacatcg
     5941 agctgtacta cactaattat ctgcacgagg agctgaaaca cgcacggggc atcctggcca
     6001 agctagagga caacaactac accggcgaca agaaggacat catccccccc ttcgccaagc
     6061 ccgaatccga caagtggaat aagaaaagca acaccatcca gtcctgcgcc gccaagtatc
     6121 tcgagcaccc cctgcagctg cctgacgggc tgttcaccca ggccatcatt gacctgctga
     6181 aggcagccga catcgacgag accatcaaga atgatgccac caaggatagt aatgccacct
     6241 acatcatccg ctattggttc gataaggtgg gccaggactc cccccagctg ttctacgaca
     6301 gcaccctgtt taagaggtcc tacaagttca ttgataagtg ctttctgcag aaagatgagg
     6361 gcaatcagac catcaaacac ttccttactg agaaggagat tctggacaat atcaagatct
     6421 tccggggaga gaccggctcc ctggccggca agctgtaccc caaggataag ctggaggagt
     6481 ctaagaagtc catcatcaac cagctgaatg aggtgaagga caaagagaag gccatccgcc
     6541 ggtatcgggt gcaggatatc gccctgtttc tgctggcccg gaagcggctg gctgagctgc
     6601 tgagtaagtc tgagaactcc aaccggaatg aagctcgcat cgagactagc ctgagccaga
     6661 acaatatcag caagaaggcc aataatctga aactgaagga tttcggattt gacgaggatt
     6721 ttcatttcct gtccggaagc gaggataatg gggacgggct gctgttcgag tacgagtaca
     6781 tcgtggataa cgatgctaat aagaagatca tgatcgtgca ggagggcctg agcctgaaga
     6841 actacggaaa tatctatagg gtgctgggcg ataaccgaat taagaccctg atgcagctgc
     6901 tgtacgagat caagaaggac aaggtcgagg acggcaccat cactttcacc ttcgccgagc
     6961 tggtgaagga gctcacaaat aatgacaagc tgcaccacga gttctacagg ctgttccaga
     7021 acattgagca ggaggcctac gatggcaccg gcggcttcga tgccaataag aagaacaaag
     7081 aggtgctgga taatcctgac gacgagcagt tctttttcgt gagacagcgg aagaacgggg
     7141 aggtgaccac caaggaggcc gtgaggaact cttttaagaa gctgatcgaa ctgctgaaac
     7201 agtatagcac agatgagggc atcatcgtgc agctggccaa tgatgccatc gccgaccgct
     7261 accctaagcc tgatgccgag atggagctga aggagaccgg cgtgaccgag agcctgatcg
     7321 cctatctgaa caacctgatc aagaagaacc agaagcagct gaagaaccag gagcagagaa
     7381 gtctagaggc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     7441 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     7501 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     7561 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     7621 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     7681 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     7741 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     7801 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     7861 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     7921 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     7981 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     8041 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     8101 tcggcatgga cgagctgtac aagtcgaatt ctaagccagg attccatatg aagcgacctg
     8161 ccgccacaaa gaaggctgga caggctaaga agaagaaaca acgcgtgcag ctaagcgact
     8221 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     8281 agtaaggatc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     8341 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     8401 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     8461 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     8521 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     8581 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     8641 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     8701 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     8761 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     8821 ggggacgtgg ttttcctttg aaaaacacga tgataaggat ccaccggagc ttaccatgac
     8881 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8941 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9001 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9061 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     9121 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9181 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9241 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9301 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9361 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9421 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     9481 atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     9541 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     9601 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9661 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9721 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9781 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9841 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     9901 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9961 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    10021 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    10081 gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
    10141 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
    10201 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10261 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10321 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10381 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
    10441 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag
    10501 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    10561 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10621 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10681 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10741 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10801 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10861 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10921 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
    10981 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    11041 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    11101 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    11161 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    11221 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    11281 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    11341 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    11401 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11461 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    11521 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11581 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11641 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    11881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    12001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    12181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    12241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    12301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    12361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    12421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    12481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    12541 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag
    12601 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    12661 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    12721 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    12781 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    12841 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12901 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12961 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    13021 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    13081 aatatgtact acaaacttag tagt
//
