LOCUS       sequence-375402-       13248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of NLS-dPba4Cas13b-EGFP-NLS in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL
  TITLE     Multi-color RNA imaging with CRISPR-Cas13b systems in living cells.
  JOURNAL   Cell Insight. 2022 Jun 15;1(4):100044. doi: 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug.
  PUBMED    37192858
REFERENCE   2  (bases 1 to 13248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Insight."; date: "2022-06-15"; volume: "1(4):100044. doi"; pages: " 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS
FEATURES             Location/Qualifiers
     source          1..13248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      7545..7554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7551..7553,7554..7556,7557..8267)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7551..7553,7554..7556,7557..8267)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7596..7617)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7857..7876)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8204..8225
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8295..8342
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8361..8426
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8433..9000
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8594..8611)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8821..8840
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9020..9619
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9020..9039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9516..9536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9638..10226
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9691..9711)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10109..10120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10301..10534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10653..10671)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10739..10843
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10844..10912,10913..11704)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11062..11081)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11875..12463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12364..12383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12617..12634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12874..12895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12910..12915,12916..12933,12934..12940)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12948..12964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12953..12975
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12972..12988
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12972..12988
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13038..13057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg atggagaagg
     3481 agaataagaa gggcgataat gacacccaga accagaataa gagcacccca cccgccaaaa
     3541 agttcatgct gatcaatgag aaccggggca agcacatcgc cggcacctat tttgagatgg
     3601 ccctggccaa catgattaga accattgagc acgtgttcat cagggcccac gtgtactcta
     3661 ttgtgggcaa gctgcaggag gacacaatgg ccgacacaat cgagcacctg gccaagcaga
     3721 atctgagtgc cacccagaag tccaccctca gcaggctgat ctttaggcac atgcccttct
     3781 tcggccccct gatggccgat gccgtggaca ctgccatcta ccccctgcgc aagaagctga
     3841 aggagctgga ggagcggcgg gacgagatca acaaggagct gcagaataag aataacaata
     3901 agagacagga gctcgaggtg gagaaacaga ccctgaaaaa gacaatcaag gccacacgca
     3961 aagccctgga gcagcagatc gtgggcagct ccccccagct gatggtggag tacctgtcca
     4021 ctatgagccg gatgatggtg accctggcca acttttattc cgccaagaat ccctacagca
     4081 acaaggagga ctatgagaaa cagaagtctg actctaagaa actggcccag tggctggaga
     4141 tcatttttaa gggcgagcgc ggcatcctgc tggagcgcaa gacacacagt caggaggaca
     4201 ccaagtttct gactcaggac ggcgtgctga attacaatgt gaacaagaac aacaagtcca
     4261 cccggaacaa cgacttctac ttcggacccg gcaaaaagac cgataagggc tgggagctga
     4321 ccgactttgg caggtacttc tttgtgagcc tcttcctgag gaaggtggac gctgatagat
     4381 tcgccaagga cgtgtgcctc tatatcgata gccccttccg gctgacagtg gaaaagaagc
     4441 tgcagctgca gcagaaggag aatctgagag ccatgaagga gcagttcatt gccgatgaaa
     4501 acggggagga ccgcatcgtc aaccccagga tcatcggcga caccgagtca tgccagaata
     4561 acatcattcg cgaaatgctg gacatgcaca ggattcacat cccacgagag aaacgcattg
     4621 acgccgagat caccgagggc atccttgtga tggacgctct gaacgagctg aggaagtgcc
     4681 ctaaggttat ccatgacacc ttctcaccta gtgataagga gaactttatc actaaaggtg
     4741 agacacctaa cggggaaatc gtggagtcca agctcgtgag accccaggac aggtttgtgg
     4801 acctggccct ccgctgcatc gaccaggagg aagtgttcac tgacatccgc ttccacctga
     4861 ggctggggct gtttagatac aggttctatc ctaaagaaac tattgacggc tcctacgtgg
     4921 agcctatccg gaccatccag aaagagatca atggcttcgg ccgcatgaac gatgtggaga
     4981 agctgcggga tgataagtac aggaccagct tccagctgcg caccattaac gaggacggcc
     5041 ttgaacagcc cgtgcccgat tcaaaggaca gccagcccta catcacagat tggaggacaa
     5101 cctacaacat ccacgccaac cgcattggcc tggcctgggg tctgcctcag atgaccggag
     5161 gctactacct gcccgatctg aacaccgatg aggaggacaa tcagaaccgc aaggccatca
     5221 ttgacatgcc ctcccctatg tgctatctga gcacttatga cctgccagcc ctgctgtttt
     5281 ataggcatat tttcaacctg taccacggca ccaagtactc caaggacggg attgtggacg
     5341 cccagacagt gattaagcag aagtacgatg ccctgcacga cttcttcatc gccgtgagct
     5401 ccagtgagct gagcaccgag gagctgctgg agaagcagaa agagctgaag attgctgatg
     5461 aggatctccc tgataagctg aggtgcttcc tgggcaccaa gaagctgttt aacaagaagg
     5521 gaaagccaat cttgaaaaag accgactcag gcgaaaaggt gcaaatcatc aaggagtgga
     5581 gggccaacga tgagaagaag aagcacgcca tcaacaccct gttggaggtc atcgaggagg
     5641 acaagttcag gctggaggcc ttcgagaaga agcactccaa gatcatcaag gggggccggg
     5701 acaatcggta cggccggcgg gggcacgccg atgtgcgcca cggcgccatt gcccgctatc
     5761 tggccaagag catgctgcgc tggcagcaga ccatcgctga ggcaggaggc ggaaagatca
     5821 cttcttccga tttccgcgtg ctgagcgact atctgacagg ctacgggatt ggcgtgcggg
     5881 aggagttcgc ctctgtggcc ctgtacaacc tgaaaaatgt tctgaagaag gctaaactga
     5941 tcgattccca gaatgagcac ccattcctga aggaaaaggt gctgtccagt tcccccatga
     6001 acattgagca gctgtacacc acctacctgc aggaggagaa aactcacgcc gaagaattga
     6061 tagccaaaat taacaatgac gacaaagaca tcgacctgcc tcccttcacc aggcccaatg
     6121 gcctgaggtg ggagacatct ctgaaagagg ccgccctgcg gtacacagag acccccgctg
     6181 ctccctttga tgagaccggc agcagccaca acgtgagcat cctgctgcct aatggcctgt
     6241 tcacccctca tatcatcaat ctgctcaaga cagccctggc cgacaaaacc attgatgagc
     6301 acaaagcact ggcacctatg agcaagctgc tggagtccaa ccccaataca ttcggcgcca
     6361 gctacatcat cagatattgg ttcgatcacg tcgcccagga cgggcctcag gccttctaca
     6421 ataacggcag ccaccagtac aagaggtacc accgggccct gtcactgctc aaccctcaca
     6481 ggaagccaaa taagcagctg atcccagact acttcaccga ggacgaaatt gccgagagac
     6541 tgaagtacgc tagacagaag aacaaggagg tgctgaagga caaggtcatc aaggctttta
     6601 atctggattc caaaaagaca gagaagattg atgagaagct gaagcagatg cgcgagatgc
     6661 tgaatgaggt cgccgccaag gagcagagca tccgccggta tagagtgcag gacattaccc
     6721 tgttcttgac cgtgagggac atgctgctga agatgctgtc ctctaaatac gatgagagga
     6781 aaaaagagaa tatcagaaaa cagaccatca aaagaatcag taagatgaga ctgcaggact
     6841 tcggctttgg ctttgacttc aagctgttcg ccgcaaccga cgacgacagt agctacacat
     6901 acgagcacaa gtctgggatc aagatcaaga tgccgatcct gtccctgaac aactacgggt
     6961 ctatctttcg gattctgagc gagggccagc gggtggataa cctgctgagc ggactgaaag
     7021 agctgggcat caccgaggtt tatttctctg acctgacagc cgagattgct gagcacgacg
     7081 agaagagaaa ggagtttctg aaaatcgccc aagacatcga gcagaaggct tatgatcaga
     7141 actatgatat cctgagtaat gacagggacc ccggcttcta taagaaagac atcatctacg
     7201 acgccaataa ccctgagcgg attctgaacc ccagcaccga cgccaagagg aaccacttca
     7261 gcgatctgat cggcttcctg aaggagttta gctccttcct gagttttaag tacgtggacg
     7321 agaacggcaa ggagaagaag atcaacctga gtgagtttgt gaaggaactg gccaacgccg
     7381 ccgcagccaa cagataccct accacatacg ccttcaagat gctgaaggag cacactaagg
     7441 gaaagaccat caaggtgcag aacatcatca atatgatcct ggagatggtg aaccaggata
     7501 tcgaggagat gaacaagaag cagagtctag aggcgctacc ggtcgccacc atggtgagca
     7561 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7621 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7681 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7741 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7801 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7861 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7921 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7981 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     8041 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     8101 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     8161 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     8221 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcg aattctaagc
     8281 caggattcca tatgaagcga cctgccgcca caaagaaggc tggacaggct aagaagaaga
     8341 aacaacgcgt gcagctaagc gactacaaag accatgacgg tgattataaa gatcatgaca
     8401 tcgattacaa ggatgacgat gacaagtaag gatccctccc ccccccctaa cgttactggc
     8461 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     8521 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     8581 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     8641 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     8701 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     8761 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     8821 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     8881 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     8941 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     9001 ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     9061 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9121 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9181 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     9241 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9301 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9361 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9421 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9481 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9541 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9601 ccgcaagccc ggtgcctgac gcccatcgat agatcctaat caacctctgg attacaaaat
     9661 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     9721 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     9781 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     9841 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     9901 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     9961 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
    10021 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
    10081 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
    10141 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
    10201 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca
    10261 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
    10321 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    10381 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10441 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10501 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
    10561 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
    10621 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
    10681 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
    10741 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10801 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10861 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10921 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    10981 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    11041 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
    11101 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    11161 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    11221 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    11281 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11341 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    11401 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    11461 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    11521 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    11581 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    11641 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    11701 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    11761 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    11821 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    11881 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    11941 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    12001 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    12061 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    12121 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    12181 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    12241 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    12301 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    12361 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    12421 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    12481 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    12541 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    12601 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    12661 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt
    12721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
    12781 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc
    12841 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    12901 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    12961 acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt
    13021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    13081 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    13141 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    13201 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
