LOCUS       sequence-375406-       12461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of NLS-dPbaCas13b-EGFP-NLS in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL
  TITLE     Multi-color RNA imaging with CRISPR-Cas13b systems in living cells.
  JOURNAL   Cell Insight. 2022 Jun 15;1(4):100044. doi: 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug.
  PUBMED    37192858
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Insight."; date: "2022-06-15"; volume: "1(4):100044. doi"; pages: " 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS
FEATURES             Location/Qualifiers
     source          1..12461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             4969..4977
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      6758..6767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6764..6766,6767..6769,6770..7480)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6764..6766,6767..6769,6770..7480)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6809..6830)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7070..7089)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7417..7438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7508..7555
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7574..7639
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7646..8213
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7807..7824)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8034..8053
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8233..8832
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8233..8252)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8729..8749
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8851..9439
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8904..8924)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9322..9333)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9514..9747
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9866..9884)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9952..10056
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10057..10125,10126..10917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10275..10294)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11088..11676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11577..11596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11830..11847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12087..12108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12123..12128,12129..12146,12147..12153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12161..12177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12166..12188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12185..12201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12185..12201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12251..12270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcgat ccatgaagat
     3481 ccccgacctg attaacaatt acaaagagca gttcggcacc tatgccgtga tggccctgat
     3541 gaacgcccag acagtgctgg accacatcca gaaactggcc tgcattggca atagcgagtc
     3601 ctacaaggag gatctgtgga aacaccctgt gatgctgcac atcaagaacg ccgcctatga
     3661 gacagataac aatcccgaga caaccctgtt tgtgatggag aagctgcagt tctactttcc
     3721 tttcctgaaa attatggccg agcggcagcg cgagtactcc aacaagaaat ataacgagaa
     3781 tcggctggcc gtgaattctc gcgatctgtt ctttgtgctg gacaaggtgt ttagagtgct
     3841 gaaaaactac gccaatacca cagccgcctt catgacaaaa tctctgagcg agaacgagga
     3901 gtatctgttt ctgaagaatg agcagccact ggccaacatg gtgaatgagt actataccgt
     3961 ggccctgcgc gatatgaaag acagatactc ctattctaca gaggatctgg ccttcatcca
     4021 ggacaacagg tacaagcaga cccggggcga cgatggcaag aggaaagcca cagtgaacct
     4081 ggacttcttt ctgtctctgc agagccacaa tggcgatccc gccaagaaac tgcacctgag
     4141 cggagtggga gtggccctgc tgatctgcct gtttctggag aagcagtacg tgaacctgtt
     4201 tatctccaag ctgtctattt tcagcaccta taaacccaat tccgagcagc accgcatcat
     4261 ttgtagatcc atgggcatcc actctattaa gctgcctaaa gatagaatcc actctctgaa
     4321 ggacggcatg agcgtggcca tggatatgct gaacgagaca aagaggtgcc ccaaagagct
     4381 gtttgataca ctgagcgccg acaagcaggc cagattcagg accatttccg acgatcacaa
     4441 tgaggtgctg ctgatgagga gctccgacag gttcgcacag ctgctgctgc agtacatcga
     4501 ttacaacaag ctgtttaaag gcattagatt ccacgtgaat atgggcaagc tgaggtacct
     4561 gtttaacgcc gccaaaaatt gtatcgacgg ccagacacgg gtgcgcgtga ttgagcacca
     4621 gctgaacggc tatggccgga tcgacgagat tgaggccgag cgcaagtccg aggatggcaa
     4681 atttgccaac tccggcatct ctattcggga tttcgagaat gtgagaaggg acgatggcaa
     4741 ccccgagaat tacccttatg tggtggacac ctactctcac tatatcctgg agaacaataa
     4801 ggtggagatg cagtttacag atagctgcat cctgcctttc attgaggagt acggcggcaa
     4861 gtggtacgtg gccaaagacg tgcctgcctg tcggatctcc accctggagc tgccagccct
     4921 ggcattccac atgctgctgc tgggctctaa gaaaacagag gagcgcatta aggaggtgta
     4981 cggcaagtat atgaaactgt tcgaggccat gtctaacgac gccgtgacca aagagaatat
     5041 cgagagcttt ggcattgccc tgtggaacct gccacagaag gccatcgatg ccattaatgg
     5101 ctgtgcccac gccaagaacg tgaatgcctt catcaaaaac accgtggagg agatgctggc
     5161 cgacacagag aagaggctgg agcggctgaa ggaggaccgg aaaggcattt ctagccacga
     5221 taacaagatg ggcaaaccaa attttcggca gatcagcacc ggccgcctgg cagatttcct
     5281 ggcaaaggac attgtgaaac tgcagcccgc caccacagag gatggcagcg acaagatgac
     5341 cggcctgaat taccgcgtga tgcaggccgc catcgccaca tataactcca atggcaaccc
     5401 tgaggccgag cagcagttta gaggcatgtt cgagaaggcc aggctgattg gcaaaagccc
     5461 aaacgcccac ccctttctgt acaaggtgtt cgcctccgcc ctgccaaaaa ataccatcga
     5521 gttctacgac aagtatctga gagagaggaa acggtatctg tctaacctga ccaaggatat
     5581 tgagagcggc aaaacagcct ccgtgccatt tgtgaataga aaccagaata agtggaagaa
     5641 acccaatcag gagtacctgg gccagaccta tggcaacagc cagaatctgg ccatcgagct
     5701 gccaaggcag atgtttgacg ccgatattaa ggccttcctg aaaggcctgc ccgagatgaa
     5761 ggacgtggat tttgacaacg ccaatgtgac attcctgatc ggcgagtaca tgaaaagagt
     5821 gctggccgac gattttcaga acttctatgc ctggaagcgc aattacagat atatggacat
     5881 gctgatgggc gagacagatt ccaaagacgg cctgcggcac atgttcacat ctgtggagga
     5941 gcgcgagaag ctgtggaggg agagagaggg acgcacctcc tcttacagaa agtgggccgc
     6001 caggaaaaac cagggctgcg gctattccaa aaggctgcct gataatgtgc tggacgagat
     6061 cctggcaaag cggctgagcg gctcccgcaa cgactaccag aagtgtgaga aagccattcg
     6121 gcgccagaag gtgcaggatg ccatgctgtt tatgctggcc aagaaagcct tcacacagca
     6181 cgccgatttt gagggcaaag acttcaagct gaaaaatatc atgcctgatg ccgacaaggg
     6241 cattctgtct gagacaatgc caatgacctt tacattcgag aagggcggca aacagtacac
     6301 aatcgagagc ggcggcatga agctgaaaaa ctatggcgac ttccacgccc tggccaatga
     6361 tagaaggatc ggctgcctga tgaagctgat tggcaccaac acagcccaga aagaggacct
     6421 gatcagcgag tttaataagt acgatcagtg tcggcctcag gtggtgaagc tggtgctgga
     6481 cctggagaag tgggccttcg ccaaataccc agagctgggc gcctccgagg agatgagcta
     6541 cgtgagcttc gacaagatcc tgaacaaact gacccagcac agaaacctgg gcaataggca
     6601 gtctaatatc ctgaggaaga ttgccaacgc cttcgacgcc aattcttacc ccgagagcgg
     6661 catcgtggag attaccacac tgcctgagat cgcccagaac atgaaagata tgtttggcag
     6721 atatgccatt gtgaagggtc tagaggcgct accggtcgcc accatggtga gcaagggcga
     6781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6841 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6901 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6961 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     7021 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     7081 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     7141 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     7201 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     7261 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     7321 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     7381 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     7441 cgccgccggg atcactctcg gcatggacga gctgtacaag tcgaattcta agccaggatt
     7501 ccatatgaag cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaacaacg
     7561 cgtgcagcta agcgactaca aagaccatga cggtgattat aaagatcatg acatcgatta
     7621 caaggatgac gatgacaagt aaggatccct cccccccccc taacgttact ggccgaagcc
     7681 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     7741 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     7801 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     7861 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     7921 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     7981 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     8041 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     8101 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     8161 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taaggatcca
     8221 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8281 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8341 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8401 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8461 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8521 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     8581 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8641 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     8701 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     8761 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     8821 cccggtgcct gacgcccatc gatagatcct aatcaacctc tggattacaa aatttgtgaa
     8881 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8941 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9001 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9061 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9121 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9181 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9241 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9301 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9361 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9421 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
     9481 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9541 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9721 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     9781 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
     9841 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9901 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9961 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    10021 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    10081 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10141 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10201 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10261 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    10321 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10381 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10441 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10501 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10561 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10621 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10681 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10741 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10801 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10861 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10921 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10981 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    11041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    11101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11221 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11461 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11881 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
    11941 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    12001 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
    12061 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    12121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    12181 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
    12241 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    12301 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    12361 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    12421 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
