LOCUS       sequence-375407-       12567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL
  TITLE     Multi-color RNA imaging with CRISPR-Cas13b systems in living cells.
  JOURNAL   Cell Insight. 2022 Jun 15;1(4):100044. doi: 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug.
  PUBMED    37192858
REFERENCE   2  (bases 1 to 12567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Insight."; date: "2022-06-15"; volume: "1(4):100044. doi"; pages: " 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS
FEATURES             Location/Qualifiers
     source          1..12567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      3975..3984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6864..6873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6870..6872,6873..6875,6876..7586)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6870..6872,6873..6875,6876..7586)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6915..6936)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7176..7195)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7523..7544
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7614..7661
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7680..7745
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7752..8319
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7913..7930)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8140..8159
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8339..8938
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8339..8358)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8835..8855
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8957..9545
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9010..9030)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9428..9439)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9620..9853
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9972..9990)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10058..10162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10163..10231,10232..11023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10381..10400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11194..11782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11683..11702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11936..11953
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12193..12214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12229..12234,12235..12252,12253..12259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12267..12283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12272..12294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12291..12307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12291..12307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12357..12376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg gaggacaacc
     3481 tgacaaaaac caagaagaag atgagaatcc ctaagctgat cgaggagcac aagtccgtgt
     3541 tcggggccta cagcaccatg gccctgagca acgtggagac agtgctgaac cacatcgccg
     3601 agagggccgg gctggacggc tacgaaagag acaggggccc tggggtggag gactactggg
     3661 agcatcccgt gatgcagtgc ctctgcagaa aggacaagcc aaggtccatc ccttctgacg
     3721 tgctgctgga cgtgcggaac aggctgttca ggagcttccc atttctgaag atcatggctg
     3781 agaaccagag ggactataga aacgctaagg gaaaagtgga gtgcgtggag atcaacgaat
     3841 ccgacatttt cgtggtgctc aataatagct ttagggtgct caaagcttac gccgatacct
     3901 gcaccgccta tctgatcgaa aaccgcattt gggaagataa ttccccaatg ctgatgtaca
     3961 acgaatgccc tctggccgcc atggtgaacc agtactacac agccgccctg agggtgacta
     4021 aggaaagata cgggtatgaa acaagggacc tgaccttcat ccagaagaga aggtttaagc
     4081 aggaaccaga gaaggaggct tccgggaacg tgaaaaagaa ggccgtgcct gacctggcct
     4141 tctttctgag cctggtggcc ctgaacgggg acggccggaa gtggctgcac ctgtccggct
     4201 ggggagtggt gctgctgatt tgcctgttcc tggagaagaa gtacgtgaat gtgtttctgt
     4261 ctaagctgcc taatccaggc aattacccac ccagtagcaa agagcggcga attatcagga
     4321 gatccatggg agtgtgcagc gtggtgctgc caaaagagag aatccactct gagaccgggg
     4381 atctgtctgt cgctctcgac atgctgaatg aactgaagag gtgtcccaga gaactgttcg
     4441 atacactctc ccccggcgat caggagcgct tcagaaccat tagcagcgat cacaacgaag
     4501 tgctccagat gcggtccaag gacagattcg cacagctggt gctgcaatac atcgatcata
     4561 accgcctgtt cgagaacttg aggttccacg tgaacatggg caagctgaga tacctcttta
     4621 atcctaagaa gtactgtatc gatggccaga ccagggtgag agtgctggag cacccgctca
     4681 atggattcgg aaggcttcag gagatggaag agaagagact gcaggagaac ggaccatttg
     4741 cccggtctgg catcaaggtg cggtgcttcg acgaggtgag gcgggacgac gctaacgagt
     4801 ccaactaccc ttatatcgtg gacacttaca cacattacgt gctggaaaac gacatggtgg
     4861 aaatgttctt ctgtcccgag gggtccggga tgaagatgcc tgaagtgacc agcagggagg
     4921 gcaagtggta tgtcgacaaa aaggtgcctc attgcagaat gagaatgtct gtgctggagc
     4981 tgcccgccat gctgttccat ctgctgctgt gcggcgccaa gaacaccgag gtgcacattg
     5041 gcaaagtgtg tgataactac tgtcacctgt ttagcgacat ggctcagggc aatctcactg
     5101 aggagaatat tctgtcctac ggcattaaga aggaagatat tccacagaag gtgtgggatt
     5161 gcgtgcgcgg ggtgcacatg ggaaaggact ctcgggccta cagggagaaa gagatccgcg
     5221 agcgctacga ggacgtgacc agacggctcg aaaggctcga ggctgaccgg aaggccgtgc
     5281 tcggcggcga aaacaagatc gggaaacgcg gatttgtcca gatcgtgccc ggcagactgg
     5341 ccgcctacct cgcaaccgac atctgcaggc tccagcctag cctcagaaag ggcgatgggt
     5401 acggaacaga tagactgacc ggcctgaatt ttaggctgct gcagtcctca attgccacat
     5461 ataattgcgg cgagagtgac atcctgtatg gaagatttcg cgacgtgttc tgttctgcag
     5521 gcctcatcgg aggcgacaat ccccatcctt ttctggataa ggtcctccca gaagcctata
     5581 gcgtgtgttg ccctcgcaat acaatcgaat tctatgagcg ctacctggag gagtaccagc
     5641 ggtacctgaa gcctcttgtg atcaagctcg aaaagggcaa agtgcctagc ctgtccttcg
     5701 tgaatgaggg ccagagaagg tgggccagaa gggacgacgc ctactatcac gaactgggca
     5761 atctgtacct gagccaggct atcgagctgc ccaggcagat gtttgacgat gagattaagg
     5821 ataagctgag agaaatgcca gagatgcggg acgtggattt tgatcacgcc aacgtgacct
     5881 tcctgatcgg agagtatctg aaaagggtcc gccacgacga aagtcaggag ttctacagct
     5941 ggccccgcca ctataagtac gtggacatgc tgaaatgtat cctgaatcca aagaacggat
     6001 ctctgcaggc cgtgtatatc cagatggggg agcgggaagg cctgtggcag gagcggagcg
     6061 aactggaaga gaagtacgcc aaaatccggc tgagagatct gggcaggaag gggctggaca
     6121 aggatgaggc taacgaaagg atcaagaccg gactcggcaa cagaaagaaa gagtaccaga
     6181 aggccgagaa agtgatccgg agatataagg tgcaggacgc ccttctgttc atgctggcca
     6241 agaacacact gtttaactcc gtggaggtgg atgatgaacg ctttaagctg aaggacatca
     6301 tgcctgacgg cgagaaaggc atcctgtccg aggtggtgcc catggacttt tgttttcggt
     6361 ctgggaacag cgccaccaga aagctgatgg gcaccatcca cagcgataac accaaaatta
     6421 agaactacgg cgacttcttc gcactggcca acgataagcg gatggtgaca ctgcttcccc
     6481 tggtgggcga acagtgtctg gtgaaagagg aggtgaaaga agagttcgat aaatacgatg
     6541 actgcagacc tgaaatgatc agtatggtgt ttgatttcga gcagtgggcc tattctgcct
     6601 accccgagct gaaagagctg gtgagcaatg aagctattaa gggacggctg ttttcaaatc
     6661 tgctgcagga gctgctgggc agaggggagc tgacatacga ggaaaagtac gcactggtgg
     6721 gaattgccaa cgcctttctg gccaattcat accctaagga tggcggagtg gtgaaggtga
     6781 ggaccctgcc tgacattgcc aagtccctga aggatgtgtt caaggagtac atccgcctgg
     6841 agagtctaga ggcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     6901 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     6961 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     7021 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     7081 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     7141 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     7201 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     7261 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     7321 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     7381 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     7441 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     7501 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     7561 ctctcggcat ggacgagctg tacaagtcga attctaagcc aggattccat atgaagcgac
     7621 ctgccgccac aaagaaggct ggacaggcta agaagaagaa acaacgcgtg cagctaagcg
     7681 actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg
     7741 acaagtaagg atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     7801 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     7861 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     7921 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     7981 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     8041 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     8101 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     8161 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     8221 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     8281 cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccaccgg agcttaccat
     8341 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     8401 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     8461 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8521 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     8581 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8641 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8701 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     8761 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     8821 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     8881 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     8941 cccatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     9001 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     9061 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     9121 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     9181 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     9241 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     9301 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     9361 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     9421 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     9481 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     9541 cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9601 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     9661 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9721 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9781 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9841 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     9901 aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg
     9961 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
    10021 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    10081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    10141 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    10201 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    10261 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    10321 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    10381 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
    10441 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    10501 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    10561 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    10621 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    10681 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
    10741 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    10801 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    10861 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    10921 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    10981 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    11041 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    11101 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    11161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    11221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    11281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    11341 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    11401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    11461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    11521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    11581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    11641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    11701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    11761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    11821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    11881 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    11941 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    12001 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
    12061 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    12121 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
    12181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    12241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    12301 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    12361 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    12421 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    12481 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    12541 ttaaatatgt actacaaact tagtagt
//
