LOCUS       sequence-375418-        4654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL63
FEATURES             Location/Qualifiers
     source          1..4654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1412..1511)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1623..1642)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1701..1720)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2294..2827)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3016..3021,3022..3038,3039..3044))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3701..4289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4190..4209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4424..4451)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4543..4629)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggtt gtaaccaatc ccaatgccaa ttcaactgag
      181 ttgatgcctt ctgagtcaag taagcttgag atcttaggta ctccggcaga taacgaagta
      241 gttgttggtg gtacggtaga gaaaacccgt ggtattgaac gaacgccgac tattttggtg
      301 aagtcgagag agtatctaat tcccgatgat gttgtggcgg ttggtggtga tattaagggg
      361 ctaagaccac cagtacttca gctccaacca gcaatgaaac tatctcatat tcctcgagga
      421 tcaacttggg atttcgttac gcatttcgct ccacctgaaa cagttgcgcc gccgagttcc
      481 tcttcagaag ccagggaaga ggaagtggaa acggaagaga cgggagctat gtttatccca
      541 ttgggggata aggagacatg ctcattcact gtaaacaagg gtgactcctc aaggacaata
      601 tctaatacgt cgccgattta tgcctccgaa ggatctttca tcacgtgttg gcagaagggt
      661 caacttctgg gacgaggatc attagggtcc gtatatgaag gcatttcagc agacggggac
      721 ttctttgctt tcaaggaagt ttcactactt gatcagggaa gtcaggcaca tgaatggata
      781 caacaagtcg agggggggat tgcgctactt agtcagcttc agcatcagaa tatcgtgcga
      841 tatcgtggca caactaagga cgagtcgaat ttgtacattt ttcttgaact tgtaacccaa
      901 gggtcccttc gaaaactcta ccaaagaaac cagcttgggg actctgtagt ctccttatac
      961 acaagacaga ttcttgatgg attgaaatat ctccacgata aaggttttat acacaggaac
     1021 attaaatgtg caaatgtatt ggtggacgct aatggaacag ttaaacttgc agattttgga
     1081 ttggctaagg ttattcttaa cccgaaggat tatgatggtt atggaactcc agctgatata
     1141 tggagccttg ggtgtactgt gctagaaatg ttgactggtc agattcccta ctccgatctg
     1201 gaaatcggta cagccttgta taacattgga acgggtaagc ttccgaaaat acctgatatt
     1261 ctatcgctag acgcccggga tttcatactt acgtgtctca aagtgaaccc ggaagagcgg
     1321 ccaactgcag ctgagctgct taaccatcca tttgtgaata tgccattacc atcctcgggc
     1381 tcaggttcag tatcttcgct cctccgtgga gacccagctt tcttgtacaa agttggcatt
     1441 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1501 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     1561 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1621 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     1681 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1741 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1801 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1861 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1921 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1981 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2041 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2101 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2161 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2221 tttctcactt gataacctta tttttgacga ggggaaatta ggccgggaag ccgatctcgg
     2281 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
     2341 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
     2401 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg
     2461 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac
     2521 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt
     2581 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt
     2641 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag
     2701 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac
     2761 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc
     2821 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     2881 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     2941 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     3001 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     3061 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     3121 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     3181 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
     3241 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     3301 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
     3361 agtggttggc tactaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3421 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3481 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3541 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3601 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4321 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4381 ataaccgtat taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4441 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4501 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4561 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4621 ttttatttga tgcctggcag ttccctactc tcgc
//
