LOCUS       sequence-375420-        3655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL112
FEATURES             Location/Qualifiers
     source          1..3655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(413..512)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(624..643)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(702..721)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1295..1828)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2017..2022,2023..2039,2040..2045))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2702..3290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3191..3210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3425..3452)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3544..3630)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggga aagaaaaacg acgaacctgc tgcttcttct
      181 tcatcatctt ctgcttctgt ggttttgcaa ctggacatgc actgtcatgg atgcaccaag
      241 aagatcctaa gaactgttca ccgtttcgat ggtgtgcagg aagttacaca tatatcctat
      301 aacaaactaa aaattgttgg aaacgtggac cctgaaatgg tgcgagataa attgcagcgt
      361 aaaattaaca aggtggttta cctcgtgaag gaaaataaac ttcatgaagt ggacccagct
      421 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
      481 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc
      541 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
      601 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
      661 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
      721 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
      781 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
      841 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
      901 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
      961 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1021 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1081 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     1141 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     1201 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     1261 aggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
     1321 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
     1381 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
     1441 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
     1501 catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag
     1561 cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca
     1621 agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact
     1681 cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac
     1741 atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct
     1801 gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     1861 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     1921 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     1981 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     2041 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     2101 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     2161 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     2221 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     2281 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     2341 caggactcta gctatagttc tagtggttgg ctactaatag gttgtattga tgttggacga
     2401 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2461 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2521 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2581 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2701 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2761 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2821 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2881 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2941 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3001 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3061 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3361 cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag tttgtagaaa
     3421 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3481 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3541 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3601 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgc
//
