LOCUS       sequence-375421-        4903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL141
FEATURES             Location/Qualifiers
     source          1..4903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1661..1760)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1872..1891)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1950..1969)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2543..3076)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3265..3270,3271..3287,3288..3293))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3950..4538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4439..4458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4673..4700)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4792..4878)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggga atttttctct ctggaaatga tattcctcac
      181 cggttagcct acgtcgcgag agaggatgaa attgtccaat ttgtagtgcc gcaaagtaat
      241 gattatataa gagggttggc tttgggcatc gtttattctt cgaaaaacga cgattctact
      301 gggtttctat ccattgatgt tgttaatctt gcccagcgaa ctagatttcg catctgggca
      361 atgaaggcca ccgtaactgc ttcccatgaa gatcatcttt ggttgggaaa tatttcaaac
      421 aagaagctca atctgaaagg cggcgacatg gttcttgtag gagcaaaatt ttatggagat
      481 aaaagaattg aggtgaacaa aacaggagta gatattgtaa agtgggattt gtctgatagt
      541 ggtactaatt gggacaccta tgtgactaag ccatataagt ctattgaaga cggtgctagg
      601 tcaagagtgt ctgatgtagg cactgatgcc gatacactat tgagctattg ccacgttttc
      661 ctctgctaca agaccccaga ggcctggcca tggcgcaaca aaggccctga gttcgatcca
      721 ctcactaggt tcttcgcatc tgctctcaaa gctcgcaaga acgacatcac cgttaagacg
      781 ttgctatcag taattgaagg acacgaagga actgagtttt ccgacgggga tgtgttgatt
      841 ttcccacaaa tgatcaaata taggggcttg aaagagtcgg atgtggatag ctttgttgat
      901 gatgtccttg tgaatgacaa accatgggct ttcggagtgc aagagccgtt gactggcccc
      961 cgtgtattta tatgcgcaca tattagtgac aatctttttt ccgcacgttt ggtcgcaggt
     1021 attctcattt ataatttcaa acatgagact aagctgcgag gattgacaga tcaggtattt
     1081 gttactgctt gctttcaaat tggaggccgc aagtatgcta gagaactcag gatctacaac
     1141 ccagattcag atggaagcat gacatgccat tggtatccct tcgtcacctt ttctgccgtg
     1201 tcgcaattgc ttgataagca tattgggaag ggagagatta tagaacgaca ttggaggggc
     1261 caaatgggag catcttctga taaagctgaa gaaattaatg accgggagct tccaaatgga
     1321 gaagaaaata agaaaatcga ggagaagcca caagaaagga atcagattca ccaagaaaag
     1381 ggcaatcaga ttgagaataa tgagaacttt tcaggctgtt gccaaggtgc taatagtgat
     1441 ggatttacat gttgcgaaga agtaagtttg gaagagaacg gtggaagtga ggaaaaaaag
     1501 ccgaaagaaa ccagagggtc gtgtgccata aaggatgcct tacggaagct gtcaagcttg
     1561 attgggaact ggaagcaaag caatgttctc gcagctgctg ctgtggttgg agcagtggca
     1621 atagtggttg tggcctatag cttttacaga aggtcacgcg acccagcttt cttgtacaaa
     1681 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1741 caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg
     1801 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1861 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     1921 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1981 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2041 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2101 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2161 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     2221 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2281 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2341 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2401 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2461 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattag gccgggaagc
     2521 cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca
     2581 cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg
     2641 cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga
     2701 gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga
     2761 tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg
     2821 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt
     2881 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa
     2941 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga
     3001 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt
     3061 tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     3121 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     3181 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     3241 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
     3301 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     3361 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     3421 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt
     3481 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa
     3541 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc
     3601 tatagttcta gtggttggct actaataggt tgtattgatg ttggacgagt cggaatcgca
     3661 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     3721 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     3781 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     3841 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     3901 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3961 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4021 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4081 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4141 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4201 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4261 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4321 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4381 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4441 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4501 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4561 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4621 attctgtgga taaccgtatt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     4681 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     4741 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4801 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4861 agcctttcgt tttatttgat gcctggcagt tccctactct cgc
//
