LOCUS       sequence-375422-        4501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL66
FEATURES             Location/Qualifiers
     source          1..4501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1259..1358)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1470..1489)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1548..1567)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2141..2674)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2863..2868,2869..2885,2886..2891))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3548..4136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4037..4056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4271..4298)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4390..4476)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgctg gcatacatag acaatgttgt acagaatttc
      181 aaggacctgc tgattgttcg ccttgtcaag ctgcctgatg tacagtctca agtggaatca
      241 gtcgtaaagg agttgagagc actgaagaag tttatcttct ttctgggaga aatatgcatt
      301 tcaacagaac aaattaaatg ctggcaaact ttctggcttc atgtcagact tgtgactacc
      361 cgtgcagcaa ttttgttata cttgccttgt tacgacagat tttctcatat gatgcaggat
      421 aacttgatgt gcagccagat agtctgcgat gacgagctcc tgaaatatat tttaaagtta
      481 ttatcattcg gcagcactca tgtcaaactg tacctcaata aagccgcaga tgaattcatg
      541 gattttcttc tgcacgtaca catgcagtta ggagcggatt catggacttc agatcaaaat
      601 tatactttta ctgaacagat caactgtttg agagctctgc tcaaagagtt accattgatt
      661 aacgacattc aggatgagat gaggatccat ttcttcaagc gactagtgac actggtcatt
      721 catgctggac ttactgtcta ctcggggggt gattgggacc aaaaaatgct tcttctctat
      781 ggcatgattc ggtccgtcaa aacagagatt tgccacaaga tccgagagtg ggtaacatct
      841 catttgccta agaatgataa attgggcttt tccaattgcc ttcttgctag cttgaaagag
      901 ttcctgtctg gtcattctgc ttcacttgct tctgtgaagg atcaaatcga agtagtccat
      961 gaggagctta agtttttcga gcctttcatc atgcgtctag cagaacaagc caataacaag
     1021 cataacgaac tccaaaatct tgttggaaga gtcattgata aggcttatga ggttgagtac
     1081 atacttgatt cttttgcaat tagtgatgta cctcttactt ttcttaggat gtggctcttg
     1141 gaaatcataa gggagattga gctcattaag actgagttga ctaaacccaa ggaaaaaaat
     1201 atgacaagtg cctcccatgc taccgacgga gaactggttg gttttacaga tgtctgtgac
     1261 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1321 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc
     1381 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1441 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1501 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1561 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1621 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1681 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1741 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1801 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1861 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     1921 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     1981 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2041 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2101 gaaattaggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     2161 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     2221 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     2281 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     2341 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     2401 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     2461 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     2521 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     2581 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     2641 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     2701 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     2761 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     2821 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     2881 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     2941 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     3001 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     3061 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     3121 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     3181 ttcgcccagg actctagcta tagttctagt ggttggctac taataggttg tattgatgtt
     3241 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     3301 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3361 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3421 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3481 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3541 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3601 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3661 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3721 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3781 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3841 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3901 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3961 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4021 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4081 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4141 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4201 ttcctgcgtt atcccctgat tctgtggata accgtattac cgctagccag gaagagtttg
     4261 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     4321 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     4381 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4441 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     4501 c
//
