LOCUS       sequence-375423-        4279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL214
FEATURES             Location/Qualifiers
     source          1..4279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1037..1136)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1248..1267)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1326..1345)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1919..2452)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2641..2646,2647..2663,2664..2669))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3326..3914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3815..3834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4049..4076)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4168..4254)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgcga caggatgatc ttcgtagtct tggtgcctta
      181 caatctctat gttgtctccg tcttaatgtg ttcaaaattg aaccagaaag gttggttgtt
      241 ggcactgagc atgcaaagtt ccactggcta gctgagtttt cgtttaccac taatgccatg
      301 gggcttatat ttagccaata ttctatgcca aggcttgaaa accttgagct agcttttaat
      361 gttcgagaga caaagtattt tgatattggc ttggagcacc tgtcctctct taaggatgtc
      421 actgcaagaa ttgattgcag ggattccagt atttttgagg tgcaaaatgc agatgctgcc
      481 attcggagga ttgcatatat gaactccaac caacctaagg ttcatgtgat caggcattat
      541 gaacacatgt taatggatga ggtgaagatc cagaaggaaa cggaggagga aaaggagata
      601 ttggaaaata ggattggtcc atggggtgga aatggtgggg tcacttgtga catcaaggtt
      661 gcacccaaac gcttggaaag cattactatt tgtagcggca taatcatcga tgcacttgca
      721 ttttcttact tggacaaaga tggagagcga cacacgacta gtttatgggg tggtcttggt
      781 ggtagtgttc aactgattga tcttgatgaa agagagtttc ttatggaggt tattggaact
      841 gtgggtccat tcaatgtgtt atctgaggct ataacatcac ttacacttgt gaccaatgta
      901 cgtagctatg ggccttttgg acaaccacag ggtactcctt tccgtactcc acggaagaaa
      961 aacagttgta tcgtgggatt ctttgggcgt tcaggaacat atcttgatgc agttggggtc
     1021 tattttcacc ctatggaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1081 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1141 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1201 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1261 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1321 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1381 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1441 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1501 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1561 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1621 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1681 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1741 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1801 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1861 ccttattttt gacgagggga aattaggccg ggaagccgat ctcggcttga acgaattgtt
     1921 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
     1981 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
     2041 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
     2101 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
     2161 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
     2221 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
     2281 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
     2341 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
     2401 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg
     2461 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     2521 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     2581 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     2641 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     2701 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     2761 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     2821 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     2881 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     2941 gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacta
     3001 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     3061 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3121 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3181 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3241 ggcgcaagct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3301 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3361 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3421 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3481 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3541 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3601 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3661 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3721 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3781 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3841 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3901 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3961 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4021 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4081 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4141 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4201 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     4261 ggcagttccc tactctcgc
//
