LOCUS       sequence-375425-        4291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL74
FEATURES             Location/Qualifiers
     source          1..4291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1049..1148)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1260..1279)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1338..1357)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1931..2464)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2653..2658,2659..2675,2676..2681))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3338..3926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3827..3846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4061..4088)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4180..4266)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgaag atccagggca taactgtgtg ctcagtgtac
      181 acaattgact ggaaagtctg gattgcgggt atcgaatttt atttgataat ccataatgag
      241 cagaagaatg tgaagctgat ttatagcccg acttgctatg gtcttcctga agcccaaaac
      301 gaaatgttat ggttcaccca tttgatattt ctgagtcagc tggatgctgg tgacaccttg
      361 aatgtctcag ttgttactat ggagggtttt agcatcaagg aaatgggtat tcacttgatg
      421 catgatgagc aagtagacat gattttgaac tcaaacagtc atgaaatgca gaaagattat
      481 ccttatcagg ggatgacgcc tgccaaacgt caagggcttg tagatttata ctgttttgtt
      541 ttcgtgaatg gaaaagtttg caaggatcca aaacttgtga cggctgatga cttcttcaga
      601 tcaggcctaa acaagcctgg aaatacttgg atcaattgtt actgcatttc tttagctcgc
      661 attgattttg cacgacgtgg tctcaatcca cctcatacac atcctagagg aactgaggtt
      721 cttgttcttc ttaagggaag actttatgtt ggtttcgtcc tttctaaccc tggcccaaat
      781 atgaagaaca agttattcac caagattctg aaccccggtg atgtatttgt ttttcctgaa
      841 ggtctacttc attttcaact taatgtggga aagagtaatg ccattgcatt tgctggtctg
      901 agcagccaaa atccaggagt tatcacaatt gcaaatgcag tatttggttc agatccacca
      961 attaaccata atgtcctcgc taaagcattt caagttgaca agaaagtgat tgatgatctt
     1021 cagcaacagt tttggtggga taataacgac ccagctttct tgtacaaagt tggcattata
     1081 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1141 attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     1201 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     1261 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     1321 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     1381 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     1441 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     1501 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     1561 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1621 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1681 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     1741 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     1801 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     1861 ctcacttgat aaccttattt ttgacgaggg gaaattaggc cgggaagccg atctcggctt
     1921 gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta
     1981 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
     2041 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa
     2101 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
     2161 gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
     2221 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
     2281 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
     2341 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
     2401 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta
     2461 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     2521 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     2581 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     2641 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc
     2701 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg
     2761 tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc
     2821 ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga
     2881 agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc
     2941 ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt
     3001 ggttggctac taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     3061 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     3121 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     3181 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     3241 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagttt tcgttccact
     3301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4021 accgtattac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     4081 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     4141 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     4201 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     4261 tatttgatgc ctggcagttc cctactctcg c
//
