LOCUS       sequence-375426-        9894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-5-TeTx::wCherry unc-54 3' UTR C.elegans B-MN and other neurons 
            expression of TeTx RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH2684
FEATURES             Location/Qualifiers
     source          1..9894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4462..5172
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4607..4625)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4875..4894
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6088..6110)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6210..6229
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6311..6329)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6397..6501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6502..6570,6571..7362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6720..6739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7533..8121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8022..8041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8275..8292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8409..8430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8445..8450,8451..8468,8469..8475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8483..8499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8488..8510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8507..8523
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8507..8523
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cggcactgtg cggaaactag caaacaattc agagccgact tccataaggt tgccaaaaaa
       61 attttagtaa tttattaatt caataattaa atgtatttat cacgaaaata aaatgaaaac
      121 atttgttgtt caaaattata tattttcaga aagaaaaaac aagtttttaa aatttaatta
      181 ctacttctca ttatgagtct ctcaaaaagc tcaacatttc agttagaata aaaattaaaa
      241 aatatcaatg aaagtgtccc gtctaattcc atttttcact ccccacactg ttcaacgtca
      301 aaacacattg tcattcagtc attttggaaa aagcaccccg agcgcagaag cagtaggaaa
      361 aacgagtctc gatgggggta ggttgaacga gcaaaaaaaa ccaattcggc taattgaact
      421 cgactttttc tcccaatttt actctcacca atcgtgatgc gtatctaaat ggatacgcaa
      481 agaaaggaaa agaaagagct ttttattttc agatccaccc acggacacgt ttttggtaga
      541 actattacat cactttttct tagttttttc tgtgagcttg atgagaactg tggtgtattt
      601 tttgtagata aaacaaaatt taaacctact tcataagttc acacacctac cttatgtcag
      661 cctagaaagt taccacgatg atggcagaaa gttgcctctt tgcctctaaa tgccgagtag
      721 tttcaaaaaa tgttgacatt tgatttggaa tttaagagtc ctctctgaaa atgtgtaatt
      781 ctgaaatttt agtgcccatt tcaaaaaaat aacatctgct cagcaaattg tatttcatcc
      841 tctctttatt ttttgttacc ccgtcttact tcctctcttt tttatttatt gttttttctt
      901 gtggtcctaa taataaaaat gaaaagagaa gctgttgtgc tcttccacaa caatgtgctc
      961 ttttcaatat tctaaaatgg caatggaatt ggcaattgtt ggagggttaa aattgccaga
     1021 caaaagtcat cttctctatg tttgttcttc atcttctttt acctctattc cttatccttc
     1081 tcaataacat cctgaaactg acgatttaat gagtatactt ccgtttatga atgtatcatg
     1141 ataatttcat gtctgaaaaa gaacggagaa atgagaatgc atattctcaa cttgaacgta
     1201 tagccaagac ttatgcctat tttcaatctg tttagaccct gacaaacatt tgaccacaca
     1261 ggagtgtttg ggacaacaac catatttttg aaaacatttt tgaatgagaa atgctatctt
     1321 ctgtgggaag ttttaaagca agctgatttc tttatatagc ccaaaataat tattttttaa
     1381 tagattatgc aaattaaaat atggagcttg atttgctgaa gtgtctattt gatttgattt
     1441 gatttgattt gatttattac gattggaatc gtgatggaat aaaagcagta tagtataaag
     1501 acagaaaaaa taaatacaca atacatagga agaataatca aaaagttacg aataaagtac
     1561 aaacaagaaa agcaattatc acctgcaaaa gttgattaat tggaacatta aagtgaacat
     1621 gaaataggct atttgggggt gtgaggagag tgggtgaatg atgggtaata agaggtgcca
     1681 atataagatt cgtcatgcta atttttcata gaactttaga ccctatttgt gaaaaagtgt
     1741 cttcttagtt tggttttgca tttcccccag atgaacttgg ttttggttct ggttttgttg
     1801 tttttatcga ttttgaaaaa ttggtccgtg tcaatgtcaa ctttatttaa ctttaagctt
     1861 taaagaataa acttatcaaa ataaatcaaa cggtactttt tttttgaaat ttttattcaa
     1921 aattcaatct tttcaagagc aaactgtaat tttttttgtc attcctatcg caaataaacg
     1981 taaaattcag ttaatttaaa atgtgagaaa tgaggtaaca actactatag aaaaataatg
     2041 aaatgttcaa gcgatgagca attaaaattt acgctcaaaa attaaccatg atgagaaaaa
     2101 aattctgctc ttcaagttat acaagactta tgagtgttaa ctttatactt caaaaaataa
     2161 attgtattga aacacctttt ttagagattt tagattatta gattgagaac tgtacaaaaa
     2221 aacgagtgtt ccaagataca tgagaatgat attttaaccc gaaacaatga aacattcaat
     2281 catacatatt ctcatccttt tcaactcgtc aacattccac gccttgtctc acttttttct
     2341 atttcccgtc cactgggatt ttgatttccc gcccgtttcg tctcggcatt ttttttttga
     2401 aagaggttac tctctcatct ttgacaccat cttccataaa acacgagaaa tgaataatgg
     2461 aatcgaggga ggttcttgtt tgtttttttt gtgtgttttt gttttgcatt ttactcatat
     2521 tttagaactt cgaaatctga tcatgactcg ttttgcgaga accattgctt taaaatattt
     2581 ttattttctt gaggataact gtttttttga aaaaaaaaac atggaatctt tggaagtatg
     2641 ctgtaaattg aggaagtctt aaaatgtttc aacttccata tgaaaaatta aatataatac
     2701 agttttgttt ccgttacaaa atccaagacg tcttctcaca aaaaatcgcc taaattttca
     2761 tttaaaaatg tgtctttgca aaagttacca gtcctgtcaa atactcgcaa caaatatcca
     2821 agaataactg actgatctga tttttgcagt tagtttttcc tcttcaaact caaaatctgt
     2881 ttttatttga accattcaat agtttaaacc ccatttgaat acgttttctc atctttcctc
     2941 gtcaccagtt tcaatgcttt aaaaacaatt ttcagcatcc cgggattggc caaaggaccc
     3001 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct
     3061 agcatgccga tcaccatcaa caacttccgt tactccgacc cggttaacaa cgacaccatc
     3121 atcatgatgg aaccgccgta ctgcaaaggt ctagacatct actacaaagc cttcaaaatc
     3181 accgaccgta tctggatcgt tccggaacgt tacgaatttg gtactaaacc ggaagacttc
     3241 aacccgccgt cctccctgat cgaaggtgct tccgaatact acgacccgaa ctacctgcgt
     3301 accgactccg acaaagaccg tttcctgcag accatggtta aactgttcaa ccgtatcaaa
     3361 aacaacgttg ctggtgaagc tctgctggac aaaatcatca acgctatccc gtacctgggt
     3421 aactcctact ccctgctgga caaattcgac accaactcca actccgtttc cttcaacctg
     3481 ctggaacagg atccgtccgg tgctaccacc aaatccgcta tgctgaccaa cctgatcatc
     3541 ttcggtccgg gtccggttct gaacaaaaac gaagttcgtg gtatcgttct gcgtgttgac
     3601 aacaaaaact acttcccgtg ccgtgacggt ttcggttcca tcatgcagat ggctttctgc
     3661 ccggaatacg taccgacctt cgacaacgtt atcgaaaaca tcacctccct gaccatcggt
     3721 aaatccaaat acttccagga cccggctctg ctgctgatgc acgaactgat ccacgttctg
     3781 cacggtctgt acggcatgca ggtttcctcc cacgaaatca tcccgtccaa acaggaaatc
     3841 tacatgcagc acacctaccc gatctccgct gaagagctct tcaccttcgg tggtcaggac
     3901 gctaacctga tctccatcga tatcaaaaac gacctgtacg aaaaaaccct gaacgactac
     3961 aaagctatcg ctaacaaact gtcccaggtt acctcctgca acgacccgaa catcgacatc
     4021 gactcctaca aacagatcta tcagcagaag tatcagtttg ataaggattc caacggccag
     4081 tatatagtga acgaggataa gtttcagata ctgtataact ccataatgta tggctttact
     4141 gagatagagc tgggcaagaa gtttaacata aagactcgct taagctattt ttccatgaac
     4201 cacgatcctg tgaagatacc taacctgctg gatgatacta tatataacga tactgagggc
     4261 tttaacatag agtccaagga tctgaagtcc gagtataagg gccagaacat gagggtgaac
     4321 actaacgcct ttaggaacgt cgacggttcc ggtctggttt ccaaactgat cggtctgtgc
     4381 aaaaaaatca tcccgccgac caacatccgt gaaaacctgt acaaccgtac cgctagggta
     4441 ccggtagaaa aaatgctcga gatggtgagc aagggcgagg aggataacat ggccatcatc
     4501 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4561 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4621 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4681 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4741 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4801 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4861 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4921 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4981 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     5041 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     5101 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     5161 ctgtacaagt aagaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt
     5221 caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg
     5281 agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact
     5341 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     5401 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     5461 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     5521 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     5581 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     5641 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     5701 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     5761 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     5821 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     5881 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     5941 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     6001 attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct
     6061 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     6121 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     6181 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     6241 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     6301 ttaattaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6361 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6421 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6481 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6601 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6661 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6721 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6781 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6841 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6901 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6961 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7021 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7081 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7141 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7201 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7261 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7321 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7381 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7441 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7501 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7561 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7621 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7681 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7741 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7801 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7861 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7921 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7981 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8041 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8101 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8161 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8221 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8281 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8341 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8401 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8461 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8521 gaccatgatt acgccaagct tgcatgcgcg gccgcatttg ttgaaaaaac gtacggtctt
     8581 catttaagag tgacatagtt tcttcttaca tttttaatat taatctgatg tttcagatct
     8641 gctctaataa tggctggttc agcacatcaa aacgagtacg acttttcgca acgacggcat
     8701 tcacttatca acatgttcaa ggcgtttaaa ttgtaaacaa tcttgtgatg cagcattgct
     8761 ccaatgataa tttgggtatt tcatagggtg ttattttgca tctgtttatc tatgaagaag
     8821 ctgaaaactg gaattatcat tagagcaagt tcgcaagcag accagcacac aaaataatgc
     8881 aacagaattt ggttcccaaa gagctcaata aagtttactt ttcacaaatg cttcaatttc
     8941 cgtttcttcc atttaaattg ttctgatgtt atagcttctc gcactttccc tccatttcaa
     9001 tttcccgcct attttctacc ctcaaaagtt cctctcatag ttttcttatt gatgaggaac
     9061 ttttgggcat tagcaaaatt gtgccctttg aattaagtta aaatgaatta aataatcttt
     9121 tattaaagcg aattcgcatc atattttaaa tgcgcggtat taataaaaat aggttcgtcg
     9181 atcaataggt gttttcatat tatccaaata cacaattggg caatttgtaa aacttgaaga
     9241 atgcggcaac gagttctgtg caagtcttta cctttaatgc ttccgtcatc gttagcaaaa
     9301 actgtgtcat gaaagacaag tgtataagat tattcaaata caattttctt atggagtttt
     9361 tctagctttt aaggatttcc aaatagaatg atcattcctt gaataataat tctctatttt
     9421 atcaatattt tgtttttttt tctattttag aaagggcgga gagtagtgtg tagtggcggt
     9481 ttcacgctct gatgatctca tgccattgtt caccttttcc aaattgtttt aggttattcg
     9541 gagtacttct tttctttctt ttccctcttc attttcaccc tctgtttgag catgagtgct
     9601 ctctattctt tggtgatgga ataaaaagag gtgccatcaa attcagaatt ttgtaaaatg
     9661 ttgaacttta gagtttttag tattcggtta gtgtgatgtt tgttttattt tcaaccctaa
     9721 caaatctcag ttttctggaa aagttgtgca tttacaatga atatattgtt ttctgcattt
     9781 cgtctgttct tagattattc agtgactttg gaaatttaag ctaattcttg ttgagccatt
     9841 tgacataatt tgtatagaaa acaaaaatgt atactttaca ccgcgattta gata
//
