LOCUS       sequence-375427-        3899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL216
FEATURES             Location/Qualifiers
     source          1..3899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(657..756)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(868..887)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(946..965)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1539..2072)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2261..2266,2267..2283,2284..2289))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2946..3534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3435..3454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3669..3696)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3788..3874)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggga gaaagcttgt gggaaacagg ggagagctac
      181 tttaatgagc ttatcaatag gagactggtt caaccggcat ttgatgatga taatgatgag
      241 ccgactggtt gttctgtcca tgctgttgtt cttgatttca tggaatcctt gtcagccgaa
      301 gaaaacttta ctactgaagg tgcaaagttg aagtgtggac tatttcctta tgaaagggtc
      361 cggcgaatct gccttgactg cggcgaagaa gacgaaggtg acgccttgtt ttgcagtaca
      421 tattgttctt tggaacaaaa gagtcaggag gatgactcaa gcagtgaaga aaactcagtt
      481 tatgcccaag acgaagcaat ttctttgcac ctatctcagg tgagatcact tgcattttgt
      541 ggggatgttg gaaggatccc tgatctttca gacttcaaac ttgtacgggt gctagatctc
      601 agtgatgcca agggcttgga aaacaaacaa ctgggaagca ttggatgttt gtcacgaccc
      661 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
      721 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg
      781 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      841 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      901 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      961 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     1021 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1081 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     1141 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     1201 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1261 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1321 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1381 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     1441 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1501 aattaggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     1561 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     1621 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     1681 ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     1741 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     1801 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     1861 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     1921 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     1981 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     2041 tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2101 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
     2161 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     2221 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
     2281 ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
     2341 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt
     2401 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac
     2461 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt
     2521 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt
     2581 cgcccaggac tctagctata gttctagtgg ttggctacta ataggttgta ttgatgttgg
     2641 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2701 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2761 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     2821 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     2881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3061 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3601 cctgcgttat cccctgattc tgtggataac cgtattaccg ctagccagga agagtttgta
     3661 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3721 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3781 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3841 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgc
//
