LOCUS       sequence-375428-        8404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Psra-11-TeTx::wCherry unc-54 3' UTR C.elegans AVB and other neurons 
            expression of TeTx RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH2633
FEATURES             Location/Qualifiers
     source          1..8404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    12..28
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17..39
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     36..52
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     36..52
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4395..5105
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4540..4558)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4808..4827
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6021..6043)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6143..6162
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6244..6262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6330..6434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6435..6503,6504..7295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6653..6672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7466..8054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7955..7974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8208..8225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8342..8363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8378..8383,8384..8401,8402..8404,1..4)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
       61 cgccaagctt gcatgcgcgg ccgcacgttt tccctttaca ttagcagaac cgaccaacga
      121 ccgaaaccta agcattatcc aggcctaaac atattttaaa aaaagtgtag tttaccaaat
      181 gggagacatt ttccttgttc tttgtgggat gcttttgaaa ttgaatagaa atctctagat
      241 taatcaatgt gtttgctccg atgcgcctag tctcaaagcc ttctcatgtt gcaaacaaat
      301 gcactaacta aactaattaa caagtcacgg aggagattca aaacatttca cttttcagga
      361 aagtagcatt tttaaatata ttcatgttgt agatttgtca ttttgcaata ttttttatca
      421 aaattggatt acaccctctt ttgagcagtc tgacttgttg gctgacttga ttttctttta
      481 aagtgatgaa attttattca tatttgattt ttagtaaata aattattgca aaccccatta
      541 tgcaactttg acagcttata tctcagttgc cgtttgtttt atgaaaaatt gtcaacttac
      601 caaataattc ttagttacga tctgaaattt ttcgaaaact ctaatcttta gtcaaaaatg
      661 ttcagcaatg taaacatttt ttcattacaa aaaaagatga tgtagatgca tacatcaagt
      721 atctcaaccg gaacaccccc tattttgcac catctctgtc atctttttct tcctttttca
      781 gatgggaaac atacaattaa tgctcacatg tcatgtctag tcatttgttt ttttttgttt
      841 gtttttaatc cgaatctctt attcatcttt agacgttcca ttcggcagag gcgagttggg
      901 gcgcctctta ctcgcctcaa attttgtgtt tgcactcata atcaaagaat aaattatctt
      961 gcccattttg gtccttgacc atttgtgtag gcaataggca ggcagacata ttttcttaaa
     1021 cagaagaccg aactgtgagt gtacaaaaat aaattaaata ttacctacct ttgttagaat
     1081 ttggaataag attaaaaaat tcgttgtcct ctgtggaatt gctcattatt agatttttac
     1141 gaaatcatgc ggattagaaa gtttaatatt tatgtacagt gcgtccaact tctatagcac
     1201 ccccctccaa ttcggccgtt tggagctttt caaaatattt ttcggaaaag tgttagtgca
     1261 gttcgatagc aaatattgaa aaacctatgc tccccaattt ttatcatgat atctcaaaaa
     1321 tcacggaaat tgggtcaaaa tatctgaaaa atggccgtcg aacttctata gcaccccctc
     1381 tgatttttga caaatttctg taaattcggg gttttcttcg tagttaatga atttattttg
     1441 taaataactt cacaccaaaa taatcaggtg gtttcacttt cacatttaaa aaataaatat
     1501 aacacttgaa aacacgtgga atgcctggga ttgatcaaaa aattgatcaa aatgttcttc
     1561 tccgtctatt cattactatg taattagttg tagaaagcat ttattcatat gaaccaaatg
     1621 gtaaagggag caacttcagt ggtttttaaa aggctcgatt taaccatgaa aacgagcgtt
     1681 cggaggcaca aagctgaagg ttttgacttc gtattgtaca gaacttcagc tcatcctgct
     1741 caaatgtctt tgaagcttcg attttgatta gatgtctttg aaaatttata cggaaaagat
     1801 catgagaccg ctcaaaaaga ggacaaaggc aaagcgaggc cgcaagtctt tttttcaagc
     1861 taacatgcga aaattttaga cagaaaagta agatatacct ttccaaatga cgattttgct
     1921 agttgtattt ataactcaag ttaaatttag cgaattcgct cattgaatct ttttttggtc
     1981 aagtaatgca aaagctacca gacaaaacat tgaacctgcg attgtagatg tctggcatct
     2041 gatttaccca tggatttcca cggatttttt gttcatataa aataattttt aatttttgct
     2101 ataatgtagt aatttttagt cgaaaatgta ttcccaacca ttatctctgc gcctccgaac
     2161 gctcgttttc atggttaaat cgaacctttt aaaaaccact gaagttgctc cctttaccat
     2221 ttggttcata tgaataaatg ctttctacaa ctaattatat agtaatgaat agacggagaa
     2281 gaacattttg atctattttg gtgctgcgaa aatcattggg agccaagata tggcctctga
     2341 agcaaataag aacgttgagg ggggtgctat agaagttgga cgcactgtat ttccaattat
     2401 tttttaagaa tttcgatttt ataaacagcc tttgctccgc ctatttgagc tcatgatgtt
     2461 tctcttttgt tttcaagatt gttgattcct tcctattgtc ctattgtgat ttttaaattt
     2521 ttaaattgct cattaacttg atttttattt agtacatatc tctaatatgt tgtttcacat
     2581 tgcaccaacc ggtctcaatt tttgaaatta tttattgtta tgtttgaata ttttaaacaa
     2641 atttaaaaat ttagaaaaat tgctgagctg ttgcctaaac tcataagtgt ttcaaaattt
     2701 gctacgattt tgcatgtact ttttaatatt ttatatgctt tttcgtattc caaaacaaac
     2761 aatttagttt actttacaaa ttatgaacaa aaaaaatttg atgtaacgga gagaaaatta
     2821 ttaaacgtta catgtatttt ttgatcgtat cgagaaaaat gcaaaaaata tcaaattgta
     2881 atttttccgc acatctccgc aacacacaat tcccgggatt ggccaaagga cccaaaggta
     2941 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gctagcatgc
     3001 cgatcaccat caacaacttc cgttactccg acccggttaa caacgacacc atcatcatga
     3061 tggaaccgcc gtactgcaaa ggtctagaca tctactacaa agccttcaaa atcaccgacc
     3121 gtatctggat cgttccggaa cgttacgaat ttggtactaa accggaagac ttcaacccgc
     3181 cgtcctccct gatcgaaggt gcttccgaat actacgaccc gaactacctg cgtaccgact
     3241 ccgacaaaga ccgtttcctg cagaccatgg ttaaactgtt caaccgtatc aaaaacaacg
     3301 ttgctggtga agctctgctg gacaaaatca tcaacgctat cccgtacctg ggtaactcct
     3361 actccctgct ggacaaattc gacaccaact ccaactccgt ttccttcaac ctgctggaac
     3421 aggatccgtc cggtgctacc accaaatccg ctatgctgac caacctgatc atcttcggtc
     3481 cgggtccggt tctgaacaaa aacgaagttc gtggtatcgt tctgcgtgtt gacaacaaaa
     3541 actacttccc gtgccgtgac ggtttcggtt ccatcatgca gatggctttc tgcccggaat
     3601 acgtaccgac cttcgacaac gttatcgaaa acatcacctc cctgaccatc ggtaaatcca
     3661 aatacttcca ggacccggct ctgctgctga tgcacgaact gatccacgtt ctgcacggtc
     3721 tgtacggcat gcaggtttcc tcccacgaaa tcatcccgtc caaacaggaa atctacatgc
     3781 agcacaccta cccgatctcc gctgaagagc tcttcacctt cggtggtcag gacgctaacc
     3841 tgatctccat cgatatcaaa aacgacctgt acgaaaaaac cctgaacgac tacaaagcta
     3901 tcgctaacaa actgtcccag gttacctcct gcaacgaccc gaacatcgac atcgactcct
     3961 acaaacagat ctatcagcag aagtatcagt ttgataagga ttccaacggc cagtatatag
     4021 tgaacgagga taagtttcag atactgtata actccataat gtatggcttt actgagatag
     4081 agctgggcaa gaagtttaac ataaagactc gcttaagcta tttttccatg aaccacgatc
     4141 ctgtgaagat acctaacctg ctggatgata ctatatataa cgatactgag ggctttaaca
     4201 tagagtccaa ggatctgaag tccgagtata agggccagaa catgagggtg aacactaacg
     4261 cctttaggaa cgtcgacggt tccggtctgg tttccaaact gatcggtctg tgcaaaaaaa
     4321 tcatcccgcc gaccaacatc cgtgaaaacc tgtacaaccg taccgctagg gtaccggtag
     4381 aaaaaatgct cgagatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     4441 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     4501 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     4561 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     4621 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     4681 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     4741 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     4801 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     4861 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     4921 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     4981 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     5041 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     5101 agtaagaatt ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
     5161 aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata
     5221 tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     5281 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac
     5341 ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
     5401 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
     5461 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
     5521 ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
     5581 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
     5641 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
     5701 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt
     5761 gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
     5821 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
     5881 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
     5941 atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt
     6001 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     6061 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     6121 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     6181 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaatta
     6241 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6301 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6361 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6421 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6481 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6541 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6601 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6661 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6721 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6781 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6841 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6901 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6961 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7021 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7081 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7141 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7201 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7261 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7321 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7381 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7441 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7501 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7561 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7621 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7681 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7741 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7801 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7861 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7921 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7981 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8041 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8101 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8161 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8221 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8281 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8341 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8401 gtat
//
