LOCUS       sequence-375429-        3991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL217
FEATURES             Location/Qualifiers
     source          1..3991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(749..848)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(960..979)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1038..1057)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1631..2164)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2353..2358,2359..2375,2376..2381))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3038..3626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3527..3546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3761..3788)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3880..3966)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggag gatgccaata tagaggatgc gttattgctt
      181 gcatcggata ttgaggatgg ggattctgct ttgcttcagg cggagatgga ggatcccatc
      241 actgtatcac taggccccat ggatcgcctc ctccgacggc tccattcatt caatgccgct
      301 gaatacaatt tgccggtggg attcactgag gataagatcc agaatctcaa agaagatctc
      361 cagcgattat gcgtttccct caaagatttg tctgaggaac aggaacctag cctgacggct
      421 aagtgcggga tgaaggaggc gcgggagctt tgttatgatg cggaggactt acttgacgat
      481 gcgacgctct gtgatattgg cgccagtgct cgcagtcgca gatcaagctc ccgtcgtctt
      541 agcatcatct cttggacccc ccgtaagctg aagcgtcgcc cccagatctc aacagattta
      601 tcagaattaa tggctcgtgt ggtggatgcg aatgatagaa gcgcaagttt cctgcttgag
      661 cataaaaccg tcaaacctgc ctttggacaa accggtgcca gccaacttcc gccgctaatc
      721 caactgcctc tcgaagtgct tgaggaagac ccagctttct tgtacaaagt tggcattata
      781 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      841 attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      901 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      961 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     1021 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     1081 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     1141 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     1201 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     1261 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1321 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1381 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     1441 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     1501 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     1561 ctcacttgat aaccttattt ttgacgaggg gaaattaggc cgggaagccg atctcggctt
     1621 gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta
     1681 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
     1741 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa
     1801 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
     1861 gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
     1921 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
     1981 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
     2041 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
     2101 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta
     2161 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     2221 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     2281 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     2341 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc
     2401 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg
     2461 tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc
     2521 ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga
     2581 agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc
     2641 ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt
     2701 ggttggctac taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2761 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2821 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2881 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2941 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagttt tcgttccact
     3001 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3061 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3721 accgtattac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     3781 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     3841 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     3901 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     3961 tatttgatgc ctggcagttc cctactctcg c
//
