LOCUS       sequence-37543-e        6449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Yang C, Tan W, Whittle C, Qiu L, Cao L, Akbarian S, Xu Z
  TITLE     The C-terminal TDP-43 fragments have a high aggregation propensity 
            and harm neurons by a dominant-negative mechanism.
  JOURNAL   PLoS One. 2010  . 5(12):e15878.
  PUBMED    21209826
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(12):e15878."
COMMENT     Sequence Label: miR-TDP43a
FEATURES             Location/Qualifiers
     source          1..6449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11..688
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(191..211)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     603..626
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           961..975
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(1642..1661)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1707..1762
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1761..1780)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2134..2156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2145..2161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2169..2175,2176..2193,2194..2199))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2214..2235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2294..2489
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2340..2475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2495..2629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2532..2551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2586..2605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2697..2714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2868..3456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2948..2967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3627..4418,4419..4487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4250..4269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4488..4592)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4623..5002
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5004..5281
                     /label=chicken beta-actin promoter
     intron          5282..6298
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6222..6244
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6306..6325
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(6370..6403)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgtcgccacc atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg
       61 catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      121 ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
      181 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
      241 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      301 gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg
      361 cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca
      421 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
      481 gaagggcgag acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
      541 caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc
      601 caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac
      661 cgagggccgc caccacctgt tcctgtaatt aattaacagg taagtgcgat cgctaatgcg
      721 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
      781 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
      841 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
      901 ccgttctgtt ggtacctgct gttgacagtg agcgaaaggt gtttgtttcg gacgttgtct
      961 gtgaagccac agatgggaca acgtccaaca aacaccttct gcctactgcc tcggacttca
     1021 agggctcgag aattcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     1081 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     1141 ctggcggccg caacaacgcg ttcctgacca ttcatcctct ttctttttcc tgcaggcttg
     1201 tggaagaaat gggaccctgc tgctgctgga gacggccact gctccctgag tgtcccgtcc
     1261 ctggggctcc tgcttcccca tcccggagtt ctcctgctcc ccacctcctg tcgtcctgcc
     1321 tggcctccag cccgagtcgt catccccgga gtccctatac agaggtcctg ccatggaacc
     1381 ttcccctccc cgtgcgctct ggggactgag cccatgacac caaacctgcc ccttggctgc
     1441 tctcggactc cctaccccaa ccccagggac tgcaggttgt gccctgtggc tgcctgcacc
     1501 ccaggaaagg agggggctca ggccatccag ccaccaccta cagcccagtg gggtcgagga
     1561 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     1621 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     1681 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     1741 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     1801 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     1861 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     1921 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     1981 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     2041 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     2101 gcccaagctt ggcgtaatca tggtcatagc tttcctgtgt gaaattgtta tccgctcaca
     2161 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     2221 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2281 tgccagcgga tccgcatctc ttttagtcag caaccatagt cccgccccta actccgccca
     2341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2461 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     2521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2581 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     2641 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3061 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3301 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3421 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3541 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3601 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3661 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3721 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3781 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3841 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3901 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3961 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4021 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4081 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4141 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4201 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4261 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4321 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4381 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4441 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4501 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4561 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
     4621 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     4681 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4741 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4801 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4861 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4921 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4981 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     5041 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     5101 gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     5161 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     5221 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     5281 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     5341 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     5401 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     5461 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     5521 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     5581 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     5641 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     5701 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     5761 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     5821 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     5881 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     5941 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     6001 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     6061 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     6121 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     6181 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     6241 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     6301 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgatcg
     6361 agggacctaa taacttcgta tagcatacat tatacgaagt tatattaagg gttccgcaag
     6421 cttcctagac tagtcgacgg tatcgcagc
//
