LOCUS       sequence-375431-        6518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses c-Jun in transfected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Mitchell JA, Lye SJ
  TITLE     Differential activation of the connexin 43 promoter by dimers of 
            activator protein-1 transcription factors in myometrial cells.
  JOURNAL   Endocrinology. 2005 Apr;146(4):2048-54. doi: 10.1210/en.2004-1066. 
            Epub 2004 Dec 23.
  PUBMED    15618352
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1210/en.2004-1066"; journalName: "Endocrinology"; date: 
            "2005-04"; volume: "146"; issue: "4"; pages: "2048-54"
COMMENT     Sequence Label: c-Jun
FEATURES             Location/Qualifiers
     source          1..6518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..95)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        80..409
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      260..395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     322..341
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             476..1270
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(530..549)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1140..1159
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1444..1565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1481..1500)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1535..1554
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1614..1630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1614..1630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1627..1649)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1638..1654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1662..1668,1669..1686,1687..1692))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1707..1728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1845..1862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2016..2604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2096..2115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2775..3566,3567..3635))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3398..3417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3636..3740)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3815..3834)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4006..4385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4386..4589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4540..4560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4634..4653
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4634..4652
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4789..4800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5880..5897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5886..6110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(6156..6518,1..66)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6243..6262)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6453..6474
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
       61 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      121 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      241 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      301 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      361 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      421 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
      481 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      541 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      601 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
      661 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      721 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      781 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      841 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      901 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      961 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     1021 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     1081 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     1141 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     1201 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     1261 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1321 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1381 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1441 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1561 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     1621 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1681 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1741 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1801 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1861 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1921 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1981 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2041 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2101 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2161 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2221 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2281 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2341 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2401 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2461 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2521 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2641 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2701 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2761 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2821 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2881 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2941 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3001 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3061 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3121 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3181 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3241 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3301 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3361 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3421 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3481 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3541 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3601 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3661 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3721 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     3781 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3841 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     3901 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     3961 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     4021 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4081 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4141 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4201 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4261 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4321 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4381 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4441 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4501 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4561 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     4621 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaact
     4681 taagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattccaaa
     4741 ctaacctcac gtgaagtgac ggactgttct atgactgcaa agatggaaac gaccttctat
     4801 gacgatgccc tcaacgcctc gttcctcccg tccgagagcg gaccttatgg ctacagtaac
     4861 cccaagatcc tgaaacagag catgaccctg aacctggccg acccagtggg gagcctgaag
     4921 ccgcacctcc gcgccaagaa ctcggacctc ctcacctcgc ccgacgtggg gctgctcaag
     4981 ctggcgtcgc ccgagctgga gcgcctgata atccagtcca gcaacgggca catcaccacc
     5041 acgccgaccc ccacccagtt cctgtgcccc aagaacgtga cagatgagca ggagggcttc
     5101 gccgagggct tcgtgcgcgc cctggccgaa ctgcacagcc agaacacgct gcccagcgtc
     5161 acgtcggcgg cgcagccggt caacggggca ggcatggtgg ctcccgcggt agcctcggtg
     5221 gcagggggca gcggcagcgg cggcttcagc gccagcctgc acagcgagcc gccggtctac
     5281 gcaaacctca gcaacttcaa cccaggcgcg ctgagcagcg gcggcggggc gccctcctac
     5341 ggcgcggccg gcctggcctt tcccgcgcaa ccccagcagc agcagcagcc gccgcaccac
     5401 ctgccccagc agatgcccgt gcagcacccg cggctgcagg ccctgaagga ggagcctcag
     5461 acagtgcccg agatgcccgg cgagacaccg cccctgtccc ccatcgacat ggagtcccag
     5521 gagcggatca aggcggagag gaagcgcatg aggaaccgca tcgctgcctc caagtgccga
     5581 aaaaggaagc tggagagaat cgcccggctg gaggaaaaag tgaaaacctt gaaagctcag
     5641 aactcggagc tggcgtccac ggccaacatg ctcagggaac aggtggcaca gcttaaacag
     5701 aaagtcatga accacgttaa cagtgggtgc caactcatgc taacgcagca gttgcaaaca
     5761 ttttgaagag agaccgtcgg gggctgaggg gcaacgaaaa aaaaaaaagg aattctgcag
     5821 atatccatca cactggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     5881 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5941 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6001 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6061 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6121 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6181 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6241 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6301 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6361 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6421 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6481 actcaaccct atctcggtct attcttttga tttataag
//
