LOCUS       sequence-375470-        7214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses c-Fos in transfected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Mitchell JA, Lye SJ
  TITLE     Differential activation of the connexin 43 promoter by dimers of 
            activator protein-1 transcription factors in myometrial cells.
  JOURNAL   Endocrinology. 2005 Apr;146(4):2048-54. doi: 10.1210/en.2004-1066. 
            Epub 2004 Dec 23.
  PUBMED    15618352
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1210/en.2004-1066"; journalName: "Endocrinology"; date: 
            "2005-04"; volume: "146"; issue: "4"; pages: "2048-54"
COMMENT     Sequence Label: c-Fos
FEATURES             Location/Qualifiers
     source          1..7214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(129..146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(300..888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(380..399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1059..1850,1851..1919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1682..1701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1920..2024)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2099..2118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2290..2669
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2670..2873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2824..2844
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2918..2937
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2918..2936
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4860..4877)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4866..5090
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5136..5564
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5223..5242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5433..5454
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5573..5593)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5578..5907
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5758..5893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5820..5839
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5974..6768
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6028..6047)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6638..6657
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6942..7063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6979..6998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7033..7052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7112..7128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7112..7128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7125..7147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7136..7152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7160..7166,7167..7184,7185..7190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(7205..7214,1..12)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
       61 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      721 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      961 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1021 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1081 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1141 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1201 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1261 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1321 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1381 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1441 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1501 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1561 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1621 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     1681 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     1741 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     1801 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1861 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     1921 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     1981 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2041 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     2101 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     2161 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     2221 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     2281 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     2341 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     2401 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2461 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2521 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2581 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2641 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2701 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2761 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2821 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     2881 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
     2941 cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt
     3001 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgaggcgca gcgaacgagc
     3061 agtgaccgtg ctcctaccca gctctgcttc acagcgccca cctgtctccg cccctcggcc
     3121 cctcgcccgg ctttgcctaa ccgccacgat gatgttctcg ggcttcaacg cagactacga
     3181 ggcgtcatcc tcccgctgca gcagcgcgtc cccggccggg gatagcctct cttactacca
     3241 ctcacccgca gactccttct ccagcatggg ctcgcctgtc aacgcgcagg acttctgcac
     3301 ggacctggcc gtctccagtg ccaacttcat tcccacggtc actgccatct cgaccagtcc
     3361 ggacctgcag tggctggtgc agcccgccct cgtctcctcc gtggccccat cgcagaccag
     3421 agcccctcac cctttcggag tccccgcccc ctccgctggg gcttactcca gggctggcgt
     3481 tgtgaagacc atgacaggag gccgagcgca gagcattggc aggaggggca aggtggaaca
     3541 gttatctcca gaagaagaag agaaaaggag aatccgaagg gaaaggaata agatggctgc
     3601 agccaaatgc cgcaaccgga ggagggagct gactgataca ctccaagcgg agacagacca
     3661 actagaagat gagaagtctg ctttgcagac cgagattgcc aacctgctga aggagaagga
     3721 aaaactagag ttcatcctgg cagctcaccg acctgcctgc aagatccctg atgacctggg
     3781 cttcccagaa gagatgtctg tggcttccct tgatctgact gggggcctgc cagaggttgc
     3841 caccccggag tctgaggagg ccttcaccct gcctctcctc aatgaccctg agcccaagcc
     3901 ctcagtggaa cctgtcaaga gcatcagcag catggagctg aagaccgagc cctttgatga
     3961 cttcctgttc ccagcatcat ccaggcccag tggctctgag acagcccgct ccgtgccaga
     4021 catggaccta tctgggtcct tctatgcagc agactgggag cctctgcaca gtggctccct
     4081 ggggatgggg cccatggcca cagagctgga gcccctgtgc actccggtgg tcacctgtac
     4141 tcccagctgc actgcttaca cgtcttcctt cgtcttcacc taccccgagg ctgactcctt
     4201 ccccagctgt gcagctgccc accgcaaggg cagcagcagc aatgagcctt cctctgactc
     4261 gctcagctca cccacgctgc tggccctgtg agggggcagg gaaggggagg cagccggcac
     4321 ccacaagtgc cactgcccga gctggtgcat tacagagagg agaaacacat cttccctaga
     4381 gggttcctgt agacctaggg aggaccttat ctgtgcgtga aacacaccag gctgtgggcc
     4441 tcaaggactt gaaagcatcc atgtgtggac tcaagtcctt acctcttccg gagatgtagc
     4501 aaaacgcatg gagtgtgtat tgttcccagt gacacttcag agagctggta gttagtagca
     4561 tgttgagcca ggcctgggtc tgtgtctctt ttctctttct ccttagtctt ctcatagcat
     4621 taactaatct attgggttca ttattggaat taacctggtg ctggatattt tcaaattgta
     4681 tctagtgcag ctgattttaa caataactac tgtgttcctg gcaatagtgt gttctgatta
     4741 gaaatgacca atattatact aagaaaagat acgactttat tttctggtag atagaaataa
     4801 atagctatat ccatgtactg tagtttttct tcaaccgcgc gtttaaaccc gctgatcagc
     4861 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4921 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4981 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5041 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5101 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     5161 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5221 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5281 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5341 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5401 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5461 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5521 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     5581 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5641 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5701 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5761 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5821 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5881 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5941 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6001 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6061 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6121 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     6181 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6241 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6301 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6361 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6421 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6481 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     6541 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6601 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6661 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6721 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     6781 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6841 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6901 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7081 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7201 cctaatgagt gagc
//
