LOCUS       sequence-375476-        6289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL156
FEATURES             Location/Qualifiers
     source          1..6289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3047..3146)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3258..3277)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3336..3355)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(3929..4462)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4651..4656,4657..4673,4674..4679))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      5336..5924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5825..5844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6059..6086)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6178..6264)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggca acacatgcaa ctacaccagc aagtgccaga
      181 ttatcgaatg ttcgatcact cttctatttt ggactgttta actgcatgcc ctcattcatg
      241 gtgtttaagc ttctccgggt tttaatcctt aatttttggg gtgatcctgg aaatacgagt
      301 ttcaacctta caggaatttg tgaactggtt tggttgagat atctgcaggt ctcatgtaat
      361 gtcaccgtaa agctaccaga tcagattgaa tcgatgggac acttggaaac actggaaata
      421 aaggcaaaag tatatgctgt tccactggac attgttcgac tttcgagctt gtcacatctc
      481 catcttcgag gcgggacaat acctaatggg attgggtgca tgagatctct acgtacactc
      541 aagtattttg acctcgtcaa taagtctgta gacaatttac ggggccttgg cgagctagca
      601 aacctgcggg atctccatct cacatattca tcatccctgc ctagtgaaca tttgaagaga
      661 aatctgatag ctctggcctc ttcacttggg aaactttgta acctcaaatc tctcactttg
      721 gcttctgaaa ctgctgccac agttgttccc tttgatggct ctattagcat gtcttctact
      781 cccgcctttc tcgagagact tgagttgtta ccatccattt gcatcttttc cagactgccc
      841 aagtggatct ttcagttcca aaaactctgc attttgaaag ttgctgttag agaactgctg
      901 gcgaatgata ttgatagcat cacaggatta ccatccctca cagttctctc attatgtgtc
      961 catacagctc ctggccaaag gatcgtcttc aacgacgggg ctttcccagt tctcaagtat
     1021 ttcaagttta ggtgtggtgt cctttccatg tctttcatgg ctggggccat gcccaatctt
     1081 cgtaggctca agttaggttt caacacccac gtcggggaga agtacggcaa tatgcttgcc
     1141 ggaattgagc accttttaaa tctccaggac attgctggaa aaattggggt tgccaccgaa
     1201 tctgacagga aggctgcaga gtctgcgatc aagggcgcca ttagcaagca tccacagagt
     1261 cctatgctta acgtacaatg ggtggatctg gttgaggaag aatatcatgc ttcagagaaa
     1321 cagcatggga ggcaagaaaa aggcttatca ggtgaaaacc acgcggtttt agagaaagct
     1381 aaagatacga agaaacatgc tgatagcggg gtgtctcaac ttcccgatct tccatcaact
     1441 gtttcttcaa gtcgcctgaa gtcagaaaac atagctcaag ctagtaaaca tcatgtcctg
     1501 gagagcatgt tatgtgatga aagcgcccag ccagaaagtc ttgaggtatc agtcttggag
     1561 gacatcacga acaatttctc tgatgatcaa gagatcagta aaaataggtt cgtagtagtt
     1621 tacaagggag agcttcaaaa tgggagtgtt gttgttgtca agaggtttac aattgcaatt
     1681 gattacaacc tatttctcga tgcggttgac tgcctgatga gtgttaagca caacaacata
     1741 gtgcgatttc taggttactg tgctaactca caagagaatg cggcaaagga aggaagcaaa
     1801 agtgtgtctg tagtgacgca agagaggttg tgctgcttcg agtatctacg caatggaaat
     1861 cttcagatgc atcttactga tgaatcttgt ggatttgact ggcaaatgtg gtatcaaata
     1921 attgaaggaa tttgtcaggg tgtgcattat cttcataaca aaagtattac tcctttagac
     1981 ctcaatccag ccaatataat gtttgatgac aaaatggtac caaaaatagt gcattatgct
     2041 tatacaagat tcttgggtga agccacaagt cctgtcctta caaatatcgg cgactcattg
     2101 gcatacatgg caccagaata cttcggtttt ggagaaatca catccaagtc tgacatatac
     2161 agtttggggg ttataattat gcagatagtg actggagaaa ataaaaagga cttcccaagc
     2221 atacaaagtg tacttgaaag ttggagaagt aggttggaat cagatggatc gcaggggcac
     2281 acatcactgg aaacatgtta ccttcaagta aatttatgct atctgatagg gataagatgc
     2341 atagagcatg acccgggaaa aagacccaat acacgggata tcatcgatag ccttgataca
     2401 ttggaaatta tggaatgttt agttaaacat gacgtaggta attcatctct agaacagatg
     2461 ttggtctgtt ctaaagttga agaaccaaaa gcattaccag ctcccccatc agtgtcttgt
     2521 aagttgaaaa tggcgatgat ggtggatcct cctaatggca cgggaaacca tgggaagcac
     2581 tactacacga tgtggcaaac catgtttgag atcgacacca agtacatgcc gatcaagccc
     2641 atcggaagag gagcttatgg gatagtttgc tcctctacaa atcaggagaa caacgagaag
     2701 gttgcgataa aaaagataaa caatgtcttt gacaaccgcg tggatgcact gaggacgctg
     2761 cgggagttga aactccttcg gcacttgcgt catgagaatg ttattgcttt gaaggacata
     2821 atgatgcctg tacataggag gagcttcaag gatgtttact tggtctttga acttatggat
     2881 actgatctgc atcagataat caagtcatct caaccgcttt ccaatgacca ctgccaatat
     2941 ttcctttttc agctactccg aggtctgaag taccttcatt cagcagggat actccataga
     3001 gacctgaagc cagggaacct tctggttaat gcaaactgtg atctggaccc agctttcttg
     3061 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     3121 atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa
     3181 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     3241 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     3301 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     3361 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     3421 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     3481 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     3541 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     3601 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     3661 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     3721 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     3781 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     3841 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaggccg
     3901 ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt
     3961 gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt
     4021 aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat
     4081 tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga
     4141 tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa
     4201 caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc
     4261 cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac
     4321 cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc
     4381 gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa
     4441 catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     4501 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     4561 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     4621 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac
     4681 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
     4741 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
     4801 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
     4861 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
     4921 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccaggac
     4981 tctagctata gttctagtgg ttggctacta ataggttgta ttgatgttgg acgagtcgga
     5041 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     5101 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     5161 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     5221 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     5281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6001 cccctgattc tgtggataac cgtattaccg ctagccagga agagtttgta gaaacgcaaa
     6061 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     6121 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     6181 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     6241 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgc
//
