LOCUS       sequence-375477-       14353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL
  TITLE     Multi-color RNA imaging with CRISPR-Cas13b systems in living cells.
  JOURNAL   Cell Insight. 2022 Jun 15;1(4):100044. doi: 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug.
  PUBMED    37192858
REFERENCE   2  (bases 1 to 14353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Insight."; date: "2022-06-15"; volume: "1(4):100044. doi"; pages: " 
            10.1016/j.cellin.2022.100044. eCollection 2022 Aug"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS
FEATURES             Location/Qualifiers
     source          1..14353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3471..3491
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7311..8024
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8043..8756
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8775..9488
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9495..9515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    9532..10105
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9699..9716)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9926..9945
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10125..10724
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10125..10144)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10621..10641
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10743..11331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10796..10816)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11214..11225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11406..11639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11758..11776)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11844..11948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11949..12017,12018..12809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12167..12186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12980..13568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13469..13488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13722..13739
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13979..14000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14015..14020,14021..14038,14039..14045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14053..14069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14058..14080
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14077..14093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14077..14093
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14143..14162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg cccaaaaaga
     3481 agaggaaagt gatggccttt gataagccta gacccaaacg gaggcaggcc aggtctgatt
     3541 tttacatcac tgacaaggct atcatggggg gctactttaa tctggcccag ctgaatttct
     3601 ttaagaccct gatggagatc ttcacaaaag ctggcatcga tgtgagcaaa atcaagcagg
     3661 ataacagccc taagtacctg atgattttga ttaagaaact gacccatgat gatgaaaaga
     3721 ttgatgacaa gaagtgggcc gatgccctgg atctgagcaa cgaatgtctc ctgaagctgc
     3781 agcagctgct ctataaacat ttccccttcc tgggaccagt gatgggcgga gaggcttcct
     3841 acaatatcta caagctgaag gacggccacc ctgaagtgaa ggtggccaat gacgtcatga
     3901 gaggcatgaa gctggaggac tgcctggctg tgctgaggca tttcgcactc tgcctgaatg
     3961 actgtgccaa cttttataca gcctttaacc catataactc tattgacgcc cagaaggagc
     4021 agtatttgag ccaaaataag gtggctgttt ggctggaaaa ggtgaaggat gcaagcagga
     4081 gaatcgtgaa gcagaacaat cagctgacct ctcaggacat ggaatttctg accggcatcg
     4141 accacatgaa gccccaggac aaggtggatg acttcggcaa catcatgacc gacaagaaag
     4201 gctacaccat taaggagtac gtggagtatg aggattatta ttttcgcatc aggggaaaac
     4261 gctatctggt ggatgcagcc ggcgccaaac tggccgatca ggaaccacga aatgccctct
     4321 ctgacttcgg cgtggtgttc ctgtgcaccc tgtttctcga aaatgatcag agcagaagaa
     4381 tgctcgacga gctgagactg ttcgagactg gtccttacga cggcagcagg gacggagatg
     4441 agttcaagaa cgacatcctg agggagattc tgctgttcta cagagcccgc atcccccgcg
     4501 gaaagaggct tgatcctatg gatgacacta cactgctcgc catggacatg ctcaacgagc
     4561 tgagaaagtg cccaatgcct ctgtacgacg tgctgtctag ggagggccag cgcttcttcg
     4621 aggacgaggt gaaacaccct aacgacctca ctcccgaggt tgccaagcgc ctcaggagtt
     4681 ccgataggtt cccatacctg gccctgcgat acattgatct gaataagtgt ttcgataaca
     4741 tccgcttcca ggtgcagtta gggaagttca ggtacaaatt ttacgataag accactattg
     4801 acggtgagca ggtggttaga ggcctccaga aggaagtgaa cgcctacggc cggttccagg
     4861 atgtggagca ctacagacag gaaaagtatg cagatatgct gcagcagact gagctggtgg
     4921 aaactggcga ggaggatatc accattgcca atttcatccc cgatactccc cagtctagcc
     4981 cctacctgag tgataggacc gctagttaca atattcacaa caaccgcatc ggcctccttt
     5041 ggaacatgcc cggagagcac gaagttctga ccggcgatga gaaaatgtac ctgcctgacc
     5101 tgaatgtgga cgacaacggc aaggccgatg tgttcctgcc cgcccctaaa gcctctctgt
     5161 ccgtcaggga cctgcccgct cttgtgtttt atctgtatct ccaaaatcag caccccgacc
     5221 tgacaccagc tgaggccatt atccagcaga agtataatgc cctggtgaga tttttcgagg
     5281 atgtcagcac aggcaggctc ctgcccgtga aaggaattgc tgagctggag gccgccatcg
     5341 acaagtatgg acacctgacc gtgcacgaaa ttccagaaaa gctgcgcgac tacctcgccg
     5401 gagtggccgg aactgaagat gacgacgact gcgccgatcg acttgactgc tacgcaatgg
     5461 gcattctgga aaagagatat agacaggtgg ccgccaggat cgaccagctg aaggaggcca
     5521 gaaagatggt tggcgacaaa atgaacaggt atggcaaaaa gtcctacatc gacgtgagac
     5581 ccaggagact ggcagagcag ctggcacagt ctatcgtgga gtggcagcca gccactgata
     5641 atggacgcca caaaatgacc gggaagaact tccagcggct gatcgccttt ctggctcgct
     5701 atatcgacga ggtgcctctc caggagctga aagacctgat gacaagggcc cgcctgctgg
     5761 aggggccagt ggcccatccc tttctgcagc aagtgttgga caagaatccc ctgaacatcg
     5821 aggtgctgta tgatctctat tttaaagagg aaaaaagata cctggacaag tacatcgata
     5881 ttgagatcaa caaacagggc gaggaggtat attggctgaa ggctgacgcc gatctgtcta
     5941 aaatcccctt cctgaatagc tctaggctga ggttccacaa acgggatagc gccagcttcc
     6001 agcgccttgc cgccaggtac ctccaggtgg acggacgcca gtccactatt ctgctcccag
     6061 atggactttt cacccctcat attctgagtg ccctgaagtc cactctgtac caggaaaacc
     6121 atgacctgca ggatcacctt caggatcagg agctgaacca gaatgcctct tatcttatgt
     6181 ccgtgtatct ggagcatgtg ctgcatgact ctgcccagcc tttttacctg tcaaacagca
     6241 atgattcccc cttcgcccgg agatatgatc tcttcgctat cgtgcagaat aagaagagag
     6301 gcgctgaact gcttccaggc cctctgaccc cagtggagat caataggcgc ctgacagcta
     6361 agacaccaga cggctccgca aagctcattg aacgtgacat cgttgccctc ctggaggaca
     6421 tggcccgcaa gaccgagcag agaattcaca agctgcccca ggatctctat ctgaaaaaga
     6481 agaagcaggc ccaggaggag cggattaagc tgcagcgccg cctgaaacac aagatcacag
     6541 atgtgaagaa gaacgagcgc gccattcgga gatacaaaac tcaggatatg gcaatcttcc
     6601 tgatggccca gcagatgctc gccaaccaac tgtcttggca gaatcaggaa ggagaggtgc
     6661 ccctgaagct ggccaacgtg tgcaatgacg gcttcctgag ccagaccatg aaggtgagct
     6721 ttccttacga agtgccactg aaagaggaca agactaagaa agtgacaatt gatattgtgc
     6781 aggacaatat gtccctgaag aactacggcc tgctgtataa gctgtaccac gacgagcgcg
     6841 tggtgacact gctgcagaac atcgtggacc agcagcagat ttctttcgcc gagctgactg
     6901 cggagctgag cctctatgac cagaacagga gcaaggcctt ctccaacttt cagcagtttg
     6961 agaaactggc ctttgaaacc aatagggaag cactgtcaaa ccccgagcat agaggattct
     7021 tcctcgaaaa ggtgaataag aaaaaccagg tcatcaggta cgagcggagg aacaattttg
     7081 atgctctcct ggacctgctg gatgccatcg aggcccacca gctggaccct cgcgatagac
     7141 agtggctgac atctgtggcc aacgcctttt gtgccaacag ctattacaag attgacatgc
     7201 agcagatcga gaaaaagctg cctaccatca tccagcaaat cgtggaaatt gtgcagagga
     7261 tcacagataa agccagccac cccagcaata ggggaggtgg atccggaagt atgcgtaaag
     7321 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
     7381 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
     7441 tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg gtaacgacgc
     7501 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
     7561 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
     7621 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
     7681 agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag ctggaataca
     7741 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
     7801 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
     7861 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
     7921 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
     7981 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatctggc ggccgctctg
     8041 gcatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg
     8101 gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg
     8161 gtaaactgac gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc
     8221 tggtaacgac gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc
     8281 agcatgactt cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct
     8341 ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg
     8401 taaaccgcat tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata
     8461 agctggaata caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg
     8521 gcattaaagc gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg
     8581 atcactacca gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact
     8641 atctgagcac gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc
     8701 tgctggagtt cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaaggta
     8761 gcatcgatgg tagcatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
     8821 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
     8881 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac
     8941 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
     9001 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
     9061 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
     9121 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata
     9181 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata
     9241 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg
     9301 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
     9361 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
     9421 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
     9481 tgtacaaact cgagccaaag aaaaagcgga aagtgtaatc tagaggatct gcccctctcc
     9541 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     9601 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     9661 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     9721 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     9781 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     9841 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     9901 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca atgggctgaa
     9961 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
    10021 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
    10081 tttcctttga aaaacacgat gataaggatc caccggagct taccatgacc gagtacaagc
    10141 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
    10201 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
    10261 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
    10321 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
    10381 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
    10441 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
    10501 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
    10561 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
    10621 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
    10681 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgccca tcgatagatc
    10741 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    10801 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    10861 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    10921 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    10981 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    11041 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    11101 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    11161 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    11221 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    11281 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
    11341 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    11401 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
    11461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    11521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    11581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    11641 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
    11701 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
    11761 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    11821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    11881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    12001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    12061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    12121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    12181 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    12241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12481 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    12721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    12781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    13021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    13081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    13141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    13201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    13261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    13321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    13381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    13441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    13501 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    13561 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    13621 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    13681 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    13741 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    13801 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    13861 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    13921 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    13981 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    14041 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    14101 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    14161 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    14221 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    14281 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    14341 caaacttagt agt
//
