LOCUS       sequence-375478-        5382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL64
FEATURES             Location/Qualifiers
     source          1..5382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2140..2239)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2351..2370)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2429..2448)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(3022..3555)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3744..3749,3750..3766,3767..3772))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4429..5017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4918..4937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5152..5179)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5271..5357)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggat ttttgtatag ctcatggagg tggtcgaaga
      181 tgcaaccatg aagattgcac acgatctgct tggggaagaa cagaattctg tgtcaagcac
      241 ggtggaggag cgagatgcaa aacatacggc tgcggaaaaa gcgctagtgg tcctttgcca
      301 ttctgccgag cccatggtgg tggtaaaaaa tgcagccatg aagattgcac aggatttgct
      361 aggggaagat caggactctg tctcatgcac ggtgggggaa agagatgcca aagagagaac
      421 tgcactaaaa gcgctgaagg tctttcggga ctctgcatat cccatggtgg tggtcggcga
      481 tgtcaatcta ttggatgcac aaaaggagcg aaagggagca aaatgttctg caaagcatgc
      541 ataactaaaa ggcctctaac gattgatgga ggaggaaata tgggaggggt aacaacaggt
      601 gatgccttga actatctcaa agctgtgaag gacaagtttg aagacagtga gaaatatgac
      661 actttccttg aagtcttgaa tgactgtaaa catcagggag ttgacactag tggcgtcata
      721 gccagattaa aagatttgtt caagggccat gacgacttac ttttgggttt taatacctac
      781 ttgtcaaagg agtaccaaat aaccattctg cccgaggatg atttccctat cgattttctt
      841 gacaaggttg agggacctta tgaaatgaca tatcagcaag ctcaaacagt tcaagccaat
      901 gccaatatgc aacctcaaac tgagtaccct tcttcctctg cggttcaatc attttcatcg
      961 ggtcaacctc agatccccac ctcagctccg gattcttcac tactagctaa aagtaatacc
     1021 tcaggtataa ctatcatcga gcacatgtca caacagcctc taaatgttga caaacaagtt
     1081 aatgatggct ataactggca aaagtatggg caaaagaaag ttaaaggcag caagtttcct
     1141 ctaagctatt acaagtgcac atatctagga tgtccttcca agaggaaggt tgagagatct
     1201 cttgatggac aagtagcaga aatcgtctac aaagatcgac acaatcacga acctcctaac
     1261 caaggaaaag atggtagcac cacatatcta agtgggagtt cgacacacat caattgcatg
     1321 agctctgaat tgacagcatc acagtttagc tccaacaaga ctaagataga gcaacaggaa
     1381 gcagcaagtc tagctacgac aatagagtac atgtctgagg caagtgacaa tgaagaagac
     1441 agtaatggag aaactagtga gggagagaaa gatgaagacg agcctgaacc aaagagaaga
     1501 attacagaag ttcaggtttc ggaactagct gatgcttcag atagaaccgt gagagagcct
     1561 agggttattt tccaaacaac gagtgaagtt gataatttag atgatggata taggtggcgg
     1621 aaatatggac agaaagttgt taaagggaat ccttatccaa ggttttcctc ctctaaagat
     1681 tatgatgtcg taatcagata cggaagagca gatataagca atgaggattt cattagccat
     1741 cttcgtgctt ccctctgccg gagagggatt tctgtctatg aaaaatttaa tgaagtggat
     1801 gcacttccaa aatgtagggt tttgattata gtattaacaa gcacatatgt cccttcgaac
     1861 ctcttaaaca ttcttgaaca ccaacataca gaggatcgag tggtttatcc aattttctac
     1921 agactatcac catatgattt tgtctgtaac agcaagaatt atgagagatt ttatctccaa
     1981 gatgagccaa aaaaatggca agctgctttg aaggaaataa ctcagatgcc tggctacaca
     2041 ttgacagata agtctgaatc tgaacttata gatgagattg taagagatgc tttaaaggtg
     2101 ctatgttctg ctgataaggt gaacatgatt gggatggaga cccagctttc ttgtacaaag
     2161 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2221 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga
     2281 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     2341 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2401 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2461 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2521 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2581 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2641 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2701 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2761 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2821 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2881 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2941 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg ccgggaagcc
     3001 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     3061 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3121 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3181 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3241 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3301 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3361 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3421 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3481 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3541 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3601 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3661 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3721 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3781 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3841 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3901 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3961 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     4021 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     4081 atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc ggaatcgcag
     4141 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     4201 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     4261 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     4321 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     4381 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4441 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4501 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4561 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4621 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4801 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5041 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5101 ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
     5161 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
     5221 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     5281 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
     5341 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gc
//
