LOCUS       sequence-375489-        3817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL200
FEATURES             Location/Qualifiers
     source          1..3817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(575..674)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(786..805)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(864..883)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1457..1990)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2179..2184,2185..2201,2202..2207))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2864..3452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3353..3372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3587..3614)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3706..3792)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgcaa gggctaaccg aagaggaagt tgcagaggca
      181 tactttaatc agctcgcaag aaggaactta atacgccctg tggagcatgg cagcaatggg
      241 aaggtaaaag cctttcaagt tcatgacatg gttcttgaat acatcatgtc gaagtcaatc
      301 gaagagaatt ttattactgt ggttggtgga cactggcaga tgactgcgcc aagcaataaa
      361 gtccgtcgac tatcgttgca aagcagtgga tccaagcatg gaaattctac aaaaggcctg
      421 aacttggctc aagtgcgatc actgacggtg tttgggaacc tgaaccatgt tccattccat
      481 tcattcaact atgggataat acaggtgctg gatcttgagg gttggaaggg tttgaaagag
      541 agacatgtga cggagatatg tcaaatgctt gtagacccag ctttcttgta caaagttggc
      601 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      661 aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta
      721 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
      781 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
      841 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
      901 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
      961 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1021 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1081 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1141 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1201 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1261 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1321 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1381 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaggccggg aagccgatct
     1441 cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt
     1501 cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca
     1561 cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg
     1621 tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac
     1681 tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt
     1741 ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg
     1801 agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa
     1861 gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca
     1921 tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     1981 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     2041 tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc
     2101 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     2161 atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc
     2221 ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca
     2281 tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg
     2341 gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag
     2401 atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt
     2461 cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt
     2521 tctagtggtt ggctactaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2581 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2641 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2701 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2761 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttttcgt
     2821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3541 tggataaccg tattaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3601 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3661 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3721 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3781 tcgttttatt tgatgcctgg cagttcccta ctctcgc
//
