LOCUS       sequence-375491-        6434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Fra-2 in transfected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Mitchell JA, Lye SJ
  TITLE     Differential activation of the connexin 43 promoter by dimers of 
            activator protein-1 transcription factors in myometrial cells.
  JOURNAL   Endocrinology. 2005 Apr;146(4):2048-54. doi: 10.1210/en.2004-1066. 
            Epub 2004 Dec 23.
  PUBMED    15618352
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1210/en.2004-1066"; journalName: "Endocrinology"; date: 
            "2005-04"; volume: "146"; issue: "4"; pages: "2048-54"
COMMENT     Sequence Label: Fra-2
FEATURES             Location/Qualifiers
     source          1..6434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..47
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(166..186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        171..500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      351..486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     413..432
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             567..1361
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(621..640)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1231..1250
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1535..1656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1572..1591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1626..1645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1705..1721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1705..1721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1718..1740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1729..1745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1753..1759,1760..1777,1778..1783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1798..1819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1936..1953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2107..2695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2187..2206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2866..3657,3658..3726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3489..3508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3727..3831)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3906..3925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4097..4476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4477..4680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4631..4651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4725..4744
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4725..4743
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5887..5904)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5893..6117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(6163..6434,1..157)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6250..6269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
       61 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
      121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
      181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
      361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
      421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
      481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
      541 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
      601 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
      661 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
      721 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
      781 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
      841 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
      901 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
      961 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     1021 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     1081 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     1141 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     1201 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     1261 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     1321 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     1381 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     1441 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     1501 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     1561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     1681 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1741 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1801 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1861 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3841 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     3901 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     3961 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     4021 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     4081 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     4141 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     4201 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     4261 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     4321 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     4381 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     4441 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     4501 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     4561 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     4621 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     4681 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
     4741 agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc ggatccaaaa
     4801 ccaccctgtt tcctctccgg cccccaccgc ggatcatgta ccaggattat cccgggaact
     4861 ttgacacctc gtcccggggc agcagcggct ctcctgcgca cgccgagtcc tactccagcg
     4921 gtggcggcgg ccagcagaag ttccgggtag atatgcctgg ctcgggcagt gccttcatcc
     4981 ccacaatcaa cgccatcacc accagccagg acctgcagtg gatggtacag cccacagtga
     5041 tcacctccat gtccaatccc tatccacgct cacatcccta cagtcccctg ccaggcctgg
     5101 cttcagtccc tgggcacatg gctctcccca gacctggagt gatcaagacc atcggtacca
     5161 ccgtgggccg cagaaggaga gatgagcagc tgtctcctga ggaggaggag aagcgtcgaa
     5221 tccggaggga gagaaacaag ctagctgcag ccaagtgtcg gaaccgtcgc cgggagctga
     5281 cagagaagct gcaggcggag accgaggagc tggaagagga gaagtctggg ctgcagaaag
     5341 agattgctga gctgcagaag gagaaggaga agctagagtt catgttggtg gctcacggcc
     5401 ccgtgtgcaa aatcagcccc gaggaacgcc gatcgccccc cacctccggg ctgcagtcct
     5461 tgcgcggtac gggcagtgcc gttggccccg tggtggtgaa gcaggagcct cccgaagagg
     5521 acagcccctc ttcctcagca gggatggaca agacccagcg ctctgtcatc aagcccatca
     5581 gcatcgccgg gggtggtttc tacggggaag agcctctgca cacccccatc gtggtgacct
     5641 ccacgcctgc catcactccc ggcacttcaa accttgtctt cacctacccc aatgtcctgg
     5701 agcaggagtc gccttcgtcg ccctcagagt cctgctccaa ggctcaccgc agaagcagta
     5761 gcagtgggga ccagtcatca gactccttga actcccccac acttctagcc ctgtaagaat
     5821 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
     5881 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5941 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6001 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6061 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     6121 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     6181 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6241 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6301 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6361 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6421 tttttcgccc tttg
//
