LOCUS       sequence-375494-        5097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses luciferase under control of Fos promoter in transfected 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Shchuka VM, Khader N, Dorogin A, Shynlova O, Mitchell JA
  TITLE     MYB and ELF3 differentially modulate labor-inducing gene expression 
            in myometrial cells.
  JOURNAL   PLoS One. 2023 Jan 3;18(1):e0271081. doi: 
            10.1371/journal.pone.0271081. eCollection 2023.
  PUBMED    36595497
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-01-3"; volume: "18(1):e0271081. doi"; pages: " 
            10.1371/journal.pone.0271081. eCollection 2023"
COMMENT     Sequence Label: pGL4.23-Fosprom
FEATURES             Location/Qualifiers
     source          1..5097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..76
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    324..445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(361..380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     415..434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(489..2141)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2193..2204
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    3096..3187
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3128..3147)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3201..3249
                     /note="synthetic polyadenylation signal"
     CDS             join(3354..3422,3423..4214)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4414..5002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4903..4922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
       61 cgagtcagtg agcgaggaag cggaagagcg ctgccggcac ctgtcctacg agttgcatga
      121 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
      181 tgactgggtt gaaggctctc aagggcatcg gtcgacggat ccttatcgat tttaccacat
      241 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      301 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      421 tgtccaaact catcaatgta tcttatcatg tctgctcgaa gcggccggcc gccccgactc
      481 tagaattatt acacggcgat cttgccgccc ttcttggcct taatgagaat ctcgcggatc
      541 ttgcgggcgt ccaacttgcc ggtcagtcct ttaggcacct cgtccacgaa cacaacacca
      601 ccgcgcagct tcttggcggt tgtaacctgg ctggccacat agtccacgat ctccttctcg
      661 gtcatggttt taccgtgttc cagcacgacg actgcggcgg gcagctcgcc ggcatcgtcg
      721 tcgggcaggc cggcgacccc ggcgtcgaag atgttggggt gttgcagcag gatgctctcc
      781 agttcggctg gggctacctg gtagcccttg tatttgatca ggctcttcag ccggtccacg
      841 atgaagaagt gctcgtcctc gtcccagtag gcgatgtcgc cgctgtgcag ccagccgtcc
      901 ttgtcgatga gagcgtttgt agcctcgggg ttgttaacgt agccgctcat gatcatgggg
      961 ccacggacgc acagctcgcc gcgctggttc acacccagtg tcttaccggt gtccaagtcc
     1021 accaccttag cctcgaagaa gggcaccacc ttgcctactg cgccaggctt gtcgtcccct
     1081 tcgggggtga tcagaatggc gctggttgtt tctgtcaggc cgtagccctg gcggatgcct
     1141 ggtaggtgga agcgtttggc cacggcctca cctacctcct tgctgagcgg cgccccgccg
     1201 ctggcgatct cgtgcaagtt gcttaggtcg tacttgtcga tgagagtgct cttagcgaag
     1261 aagctaaata gtgtgggcac cagcagggca gattgaatct tatagtcttg caagctgcgc
     1321 aagaatagct cctcctcgaa gcggtacatg agcacgaccc gaaagccgca gatcaagtag
     1381 cccagcgtgg tgaacatgcc gaagccgtgg tgaaatggca ccacgctgag gatagcggtg
     1441 tcggggatga tctggttgcc gaagatgggg tcgcgggcat gactgaatcg gacacaagcg
     1501 gtgcggtgcg gtagggctac gcccttgggc aatccggtac tgccactact gttcatgatc
     1561 agggcgatgg ttttgtcccg gtcgaagctc tcgggcacga agtcgtactc gttgaagccg
     1621 ggtggcaaat gggaagtcac gaaggtgtac atgctttgga agccctggta gtcggtcttg
     1681 ctatccatga tgatgatctt ttgtatgatc ggtagcttct tttgcacgtt gaggatcttt
     1741 tgcagccctt tcttgctcac gaatacgacg gtgggctggc tgatgcccat gctgttcagc
     1801 agctcgcgct cgttgtagat gtcgttagct ggggccacag ccacaccgat gaacagggca
     1861 cccaacacgg gcatgaagaa ctgcaagcta ttctcgctgc acaccacgat ccgatggttt
     1921 gtattcagcc catagcgctt catagcttct gccagccgaa cgctcatctc gaagtactcg
     1981 gcgtaggtaa tgtccacctc gatatgtgcg tcggtaaagg cgatggtgcc gggcaccagg
     2041 gcgtagcgct tcatggcttt gtgcagctgc tcgccggcgg tcccgtcttc gagtgggtag
     2101 aatggcgctg ggcccttctt aatgtttttg gcatcttcca tggggtcgaa gtttggggaa
     2161 agcccggcaa ggggtccagg ggtagacact ggtgggagct gcagagcaga gctgggtggg
     2221 agcgcggtca ctgctcgttc gcggaaccgc cggctctatc cagtcttctc agttgctcgc
     2281 tgcagtcgcg gttggagtag taggcgcctc agctggcgcc tttatagaag cgctgtgaat
     2341 ggatggactt cctacgtcac tgggcggaac tggaaggggg gggaagcgcg ccccaaggcc
     2401 ctctgcgggc gctctgtcgt caactctacg ccccaggctt tcaacccccg tcttggcata
     2461 catctttcac ctatgagtgt tcacatttgg gatcttaggg ggtctcctag accttccgcg
     2521 tgtaggattt cggagatggt ccccccccca gaacaacagg gaccggccgt ggaaacctgc
     2581 tgacgcagat gtcctaatat ggacatcctg tgtaaaggag ggagggattg acgggaacag
     2641 ctcgccggct gcagccaact ctgagggtgc agcgcagggg gggaaccggg gccgcggccg
     2701 ggggagggga ggcgggaaag gcagagaagg cgagcgaaca ttcgcacctg attcaatgtg
     2761 gacccggttc ccagggtcaa gggatgagac ccgggtcatt gtccagcaat ctggaacttt
     2821 tttttttttt ttgagtctct ggtattagga gaagcaagta cgcagcctta aaggctcgaa
     2881 gcctctgttc ctaaaggaaa gcttgcccac atcagccccc catcccaaat cagtgcagag
     2941 aagaaaaagg gggggtcagc ttctcagccc ctctctccag ctccttgcct ggagaaactt
     3001 taggactgag gttggaagca gcaagtgaag gattgtaaat aagtagatct tgatatcctc
     3061 gaggctagcg agctcaggta ccggccagtt aggccagaga aatgttctgg cacctgcact
     3121 tgcactgggg acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc
     3181 gtatgttagt actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga
     3241 tattttattg cggcctgtcc aatacctccc gtaccttaat attacttact tatccttgag
     3301 agacgtacta gtaaccctga taaatgcttc aataatattg aaaaaggacg agtatgagca
     3361 tccaacattt tcgtgtcgca ctcattccct tctttgcggc attttgcttg cctgtttttg
     3421 cacaccccga aacgctggtg aaagtaaaag atgctgaaga tcaactgggt gcaagagtgg
     3481 gctatatcga actggatctc aatagcggca agatccttga gtctttccgc cccgaagaac
     3541 gattcccgat gatgagcact ttcaaagtac tgctatgtgg cgcggtgttg tcccgtatag
     3601 acgccgggca agagcagctt ggtcgccgta tacactactc acaaaacgac ttggttgagt
     3661 actcgccggt cacggaaaag catcttacgg atggcatgac ggtaagagaa ttgtgtagtg
     3721 ctgccattac catgagcgac aacaccgcgg ccaacttact tctgacaacg atcggaggcc
     3781 ctaaggagct gactgcattt cttcataata tgggtgatca tgtgacccgg cttgaccgct
     3841 gggaaccaga gttgaacgaa gccataccga acgacgagcg tgataccacg atgccagtag
     3901 caatggccac aactcttcgg aaactactca ctggcgaact tcttactcta gcatcacgac
     3961 agcagctcat agactggatg gaggcggaca aagtagcagg accacttctt cgctcggccc
     4021 tccctgctgg ctggttcatt gctgacaaat cgggggccgg tgaacgcggc tctcgcggca
     4081 tcattgctgc gctggggcct gatggtaagc cctcacgaat cgtagtgatc tacacgacgg
     4141 ggagtcaggc cactatggac gaacgaaata ggcagatcgc tgagatcggt gcctcactga
     4201 tcaagcactg gtaaccactg cagtggttta gcatttgcgg ccgctgtcag accaagttta
     4261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataa
//
