LOCUS       sequence-375497-        4639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL70
FEATURES             Location/Qualifiers
     source          1..4639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1397..1496)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1608..1627)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1686..1705)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2279..2812)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3001..3006,3007..3023,3024..3029))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3686..4274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4175..4194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4409..4436)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4568..4611)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgcaa tatattattg acaatctgct actgaagcat
      181 gaaactgatt tgtcatttac agttgcagat gatgttaaaa agttctatga tggattgttg
      241 ctcctgttaa ctcatcttgt tgatactcta tctcagtgca gtgaatttgg aactactgca
      301 attgaggcca aatctgcgaa tagcaacaaa agcaatctaa ttcttcagtt tttgacggtg
      361 gctttcaagc ttatcggatg tgagagaagt ttgatggatc tacagaagca acacacagtc
      421 cataatgtct taatgcatgc tgaagtgact gccaacaaga tagcacaaat catcaaaaga
      481 agaagcactg aggaaattgg gcttttatta tctgagattg agtctgtcaa ggtagagatc
      541 agaaaagtat gctttcaatt tctggatgca tcaccttaca acatgacaga tggcgaagac
      601 cttattagat tcttatcgaa acaccaggac tggctgctca actttgatgc cttttcaatt
      661 ccttttttga agaaacagat tccagaaatc aaaggcaaac tattttattt gggttctttt
      721 attgcagata ttgtacagca tcgtgatatg catcaagagc tcaaagatct tgtgaaacgt
      781 gttcaagata taaagtttgt ctgtctcttt cccatcaggg attctgcacc ttcttggtgt
      841 tacaggttat atctctctga tgtcaagcaa ttgcttaagt ttgtcgagac agaggtcgaa
      901 atgatttgtc tcaaagttcc agatttttca agtcatagct tccccaagat aaatgaacta
      961 ggatttcttg attgcttcct gggaaaattg gatgagatgt taagttctaa gcttgagtca
     1021 gtcatcgact taaaatgcca aattgagcca ttgaaggagg gcttattgtg tctaagaacg
     1081 ttgactgatc atttcccaga aatctacgat gagcatgatg aagtttatag tcttataaca
     1141 agagttaccg ccatggcata caaggcagag tatgttatgg actcgtgttt gacctattct
     1201 tatccactct ggtacaaagt ttgttggatt tctgaagctg ttgagaatat taagcttgta
     1261 aatgaagttg ttagggaaac atgtgaaaga aaaaaggtag atgtcgcagt gctcaaaatt
     1321 agaaagacct caattcatct tgtgccgtct ttatcatcta atactccagg atcagatgaa
     1381 gaaatggaga gtttcgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1441 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1501 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1561 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1621 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1681 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1741 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1801 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1861 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1921 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1981 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2041 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2101 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2161 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2221 ccttattttt gacgagggga aattaggccg ggaagccgat ctcggcttga acgaattgtt
     2281 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
     2341 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
     2401 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
     2461 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
     2521 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
     2581 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
     2641 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
     2701 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
     2761 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg
     2821 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     2881 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     2941 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     3001 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     3061 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     3121 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     3181 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     3241 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     3301 gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacta
     3361 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     3421 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3481 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3541 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3601 ggcgcaagct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3661 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3721 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3781 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3841 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3901 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3961 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4021 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4081 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4141 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4201 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4261 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4321 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4381 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4441 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4501 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4561 acaacagata aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tttgatgcct
     4621 ggcagttccc tactctcgc
//
