LOCUS       sequence-375500-        4105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL202
FEATURES             Location/Qualifiers
     source          1..4105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(863..962)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1074..1093)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1152..1171)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1745..2278)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2467..2472,2473..2489,2490..2495))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3152..3740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3641..3660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3875..3902)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3994..4080)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgggg gaacagtatg gtgatattct taccggcatt
      181 gaacacctgt tgaaccttca agagatcatt ggacaaattg gagctgctgc gggtgcagag
      241 gaatctgaca agagagccgc tgagtttgcg ttaagtgatg ccactaagaa gcattcgagg
      301 atttctagat tcaacatacg atggattaat tgtgttgaag aagatgtcag ggtttccaag
      361 atttcagatt tacaaggaat atcttcaagt tcagatgaag aaaagcaaaa ggctaatgag
      421 ccaagtggga cagatattac gattgaggac gagtcgccat tttctcacga gccaaattat
      481 gatgttccac gggcatacaa ggcagagata gaaagcgaag aagttcctca cgcggaatta
      541 gcagatgcct ctcgcacaag cgattcaatc gacaatcgtc gacggcaacg ccggtcaatc
      601 gagccttctt tctcccgcga tcgcctcagc cagccgagga agcactcggt cgcgtccgcc
      661 gtgccaaagt tcggcgagtg ggactcgccg ctccgcccgg cagacaacta cacaagcatc
      721 tttaacaggg cgagggaaga aaagcgtagt caggtagaga agccgtcggt gtttgacaag
      781 gatgacctac tggttagaag aaccagaaat cgcaacgacg gccacgcctc cacgaaatgc
      841 tcctgctttg gatggtgcaa agacccagct ttcttgtaca aagttggcat tataagaaag
      901 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      961 tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1021 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     1081 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     1141 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1201 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1261 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     1321 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     1381 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     1441 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     1501 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     1561 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     1621 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     1681 tgataacctt atttttgacg aggggaaatt aggccgggaa gccgatctcg gcttgaacga
     1741 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
     1801 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
     1861 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
     1921 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
     1981 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
     2041 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat
     2101 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
     2161 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
     2221 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg
     2281 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     2341 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     2401 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     2461 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
     2521 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg
     2581 ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg
     2641 ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct
     2701 cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt
     2761 tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc tagtggttgg
     2821 ctactaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2881 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2941 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3001 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3061 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3841 ttaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3901 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3961 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     4021 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     4081 atgcctggca gttccctact ctcgc
//
