LOCUS       sequence-375501-        5218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL71
FEATURES             Location/Qualifiers
     source          1..5218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1976..2075)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2187..2206)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2265..2284)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2858..3391)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3580..3585,3586..3602,3603..3608))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4265..4853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4754..4773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4988..5015)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5107..5193)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgatg gaagatgaat gcaattcatt ctatacattc
      181 ttagttgaat atctgttttc agaggaactt gataccctta gcgttctgct gcgtattgaa
      241 gaggactggg gctcattccg tatgtatttg gaggaattca gatcaaaggt gtctgatttc
      301 gttgaaactt cacaggaatg tagagaggga tgtgattcat tcattatact tttagaaaca
      361 tatttgttgt cctcagagga ttatctgctg ttgatgcaag atgaatggag ttccttcgat
      421 ttcttttcag aagaacatat gtctttgttt gatagactga ggctccttaa aagggattcc
      481 aacttcctga gtatcattat cgaattgcat atcttcaaag atcaaccaga tgtcagctgg
      541 ggaaaagttc gagcattgtt ccacggtgct gcagatgaac tcataaagat gtacagtacg
      601 gaagtcagtc cgtggaatca ccaattttat gactatatct gccacttgca atatgagttt
      661 cagcagacta agttggaaat catgagggct aatttcccct ttcccaaaat atcatgtcaa
      721 cttttagcca atgaggatgg tattgtgatt cccggttctg taatgaaatt cattgacatt
      781 gttgctgata gtctcagtaa tttactgaaa tttgacaatc caagttcact actttgtatt
      841 gggggacgat gcatggttca aatagaaatg gttttgaagg ggttggattt cctctctagt
      901 tttgtctgct ttgtttcaga tagatgcata gagactcggg tccaacatgc tttgttcact
      961 catgttgtac aagtggcctg gcaaacaact atggctacgt ggttgtatct tccgagcaat
     1021 gaatacatgt atcaagacac agctccaaac ggtgagaatc ctttgctttc tgatcttctg
     1081 cagaggaaaa tccagcttat tcagccaagc atatgcaagt tctatattca tatcttgcaa
     1141 gctctaaatt tggttgagtc acaatggaat cccgttatca atgttaagta tgtattcgat
     1201 tgtgaagttg gttttctgga gtctcttcga cgcactttaa aaggattacc agtctctagt
     1261 aattgcattg caataaaggc agaacttcag gagacgctca acttcttcgg agatactcta
     1321 gtcaatctac caacacaggt cattgaactt catcttcagg atatagactt ttcaattgtt
     1381 gatgcaggac ttctggtttg ctcattaaat gatgataatg agaatcttga tttcacggga
     1441 aaaatccaaa gtatgcaaag tgtgatctac ctcatcgcta gaaagaaatt cctcctccag
     1501 ttcaacttac ctggaattga cagagtggac tctgctaatt tcgttttaga caacagggag
     1561 aagtttctga gcatgtattc aaattcagtt gattcggttc aaagccaact tccgaaaatt
     1621 cagaaagaac tccagttctt tcaagctgtt gtacaacagc aagacggact tcaacatttt
     1681 gcaacgaaga cttctcattt ggtgtatgag gtagaacata tggttgatac ctgtaagaaa
     1741 aaagatgttc cggactggtg tctttttatc tggatcttga atattggaga ggatataaga
     1801 atgctcatgg cagaagtagc agagatagca gagattccta atgagttact ctcatctcca
     1861 aacaagctaa cttcatttgt gcagttagtt ctcaagggat ttgttcggat ctttggtgtt
     1921 gcttcttcac aattagctag taaacaaagg atcaatgaag aaatcgtagg ctttgaccca
     1981 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2041 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc tctggcccgt
     2101 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     2161 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     2221 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     2281 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     2341 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     2401 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     2461 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     2521 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     2581 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     2641 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     2701 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     2761 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     2821 attaggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
     2881 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     2941 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     3001 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     3061 gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga
     3121 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     3181 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     3241 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     3301 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     3361 gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     3421 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
     3481 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     3541 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
     3601 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
     3661 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
     3721 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
     3781 gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg
     3841 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
     3901 gcccaggact ctagctatag ttctagtggt tggctactaa taggttgtat tgatgttgga
     3961 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     4021 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     4081 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     4141 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     4201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4381 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4921 ctgcgttatc ccctgattct gtggataacc gtattaccgc tagccaggaa gagtttgtag
     4981 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     5041 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     5101 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     5161 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgc
//
