LOCUS       sequence-375506-        4372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL215
FEATURES             Location/Qualifiers
     source          1..4372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1130..1229)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1341..1360)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1419..1438)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2012..2545)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2734..2739,2740..2756,2757..2762))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3419..4007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3908..3927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4142..4169)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4261..4347)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggaa cctggaagca gcagtaggga gggggagcag
      181 caggagccag caaacgaacg actaaccgcc atcggaggag gaggaggagg aggaggagga
      241 agccctgtct cagatcagga actctcggat ggtgaggaag tgtctgatgg tgagtatcag
      301 gccggcgacg atttctctgg ctacgcggtc cggggccggg ggttcgtcga gaaggagcac
      361 atattcgaca aggtggtgac gcccagcgac gtcgggaatc tgggccggct ggtgatcccg
      421 tggcagcacg cggagtgcta cttcccgcgg gacgtacccg cgaacgagag ggagggcgtc
      481 gtcctcagat tcgaggacga tgccggcaat tcatggcgtt tcctctaccg cggcagcagc
      541 ttgacgttgg gatggagcca cttcttcagg aagaaccgcc tcgacgccgg ggacatggtc
      601 tccttctacc gtggcgccag cgaggccacc cgtgaccgcc tcttcatcca ctcgaagcgc
      661 cggatgcaca ttctccctac tctaggctac tcagaccctc aggttcatat caatcgcctc
      721 tttcagctgc tggttcgggt aagaaccatg gtttcatcct tcggtaaatc tgggtcgccg
      781 tcgccgccca attctccgct tcctacagtt caaaatttca cctacaaagc gctaaaagat
      841 tgcctccaag atctgcacta ctacagcctc ctgtccatga tggggagaaa ccacaaaagt
      901 cagtcaacat ccgcaaagat ccgtgatact attcctacgc ttgatgcttt gatacaatta
      961 cgaagcctgt cagatccagt ttttcgtatt cccgccgcag cgacgtcgca ttgcatcctc
     1021 ttatgccgtg gtgttttggg gatcatgggc ttcctatgct ctgatgagaa tatgaaggag
     1081 gatcaccgga tgcttcaggt tcctgctttt gatgatctga actactctga cccagctttc
     1141 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1201 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca
     1261 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     1321 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     1381 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     1441 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     1501 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     1561 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     1621 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     1681 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     1741 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     1801 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     1861 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     1921 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg
     1981 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     2041 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     2101 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     2161 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     2221 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     2281 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     2341 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     2401 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     2461 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     2521 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     2581 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     2641 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     2701 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     2761 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     2821 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
     2881 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
     2941 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
     3001 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
     3061 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc
     3121 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3181 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3241 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3301 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3361 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3421 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3481 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3541 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3601 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3661 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3721 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3781 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3841 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3901 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3961 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4021 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4081 tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc
     4141 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     4201 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     4261 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     4321 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gc
//
