LOCUS       sequence-375509-        4123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL226
FEATURES             Location/Qualifiers
     source          1..4123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(881..980)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1092..1111)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1170..1189)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1763..2296)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2485..2490,2491..2507,2508..2513))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3170..3758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3659..3678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3893..3920)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4012..4098)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggat tctggtaata ttggagggag tctccagtcg
      181 tcgagcggcg gcgacgacga gttcgattcg cggggaggtg gtggtggggt ggagggggcg
      241 ggggcggcag tggccgcggc gccggcgacg gcgaggaggt cgaggaagcg gacgagggcg
      301 tcgtggcgga cggtgaccac cgacccctcc aacttccgcg ccatggtgca ggagttcacc
      361 gggatcccct cgccgccgtt cgtcgcgggc gtcggggcac ccgccgcctc gctccgcacg
      421 cgcttcgacc acctcttccc ctcgccggcg tccgccctcc gctccgccgc cgccggcgat
      481 ccggcatcct cgctgccgcc gtacctcctc cgccccatcg cgcagaagct ccccacggct
      541 tcggcgcaga acagcaccat gcagcagact cgtgatccca gcgcacttcc gcattcatcc
      601 agcttcgtca catggacgcc ggacgaattt cattcacccg gtttcaactc atcgaaacag
      661 gatgaacggt ttgtgctcga gaacactctc cgtggtatgt acgaacttat taagctcgcg
      721 gagtgttggg ttcgcaagga ttattccatt gctaatctcc tccaagaagc caaggataca
      781 gtgtactgtg ctgaagatct cttggacgag ctcaattact atgagctcca ggacggagtt
      841 gggttcagtg cgaatagatc ttcatgccca gagttttctg acccagcttt cttgtacaaa
      901 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      961 caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg
     1021 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1081 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     1141 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1201 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1261 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     1321 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     1381 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     1441 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     1501 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     1561 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     1621 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     1681 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattag gccgggaagc
     1741 cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca
     1801 cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg
     1861 cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga
     1921 gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga
     1981 tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg
     2041 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt
     2101 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa
     2161 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga
     2221 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt
     2281 tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     2341 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     2401 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     2461 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
     2521 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     2581 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     2641 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt
     2701 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa
     2761 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc
     2821 tatagttcta gtggttggct actaataggt tgtattgatg ttggacgagt cggaatcgca
     2881 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2941 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     3001 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     3061 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     3121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3301 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3841 attctgtgga taaccgtatt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3901 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3961 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4021 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4081 agcctttcgt tttatttgat gcctggcagt tccctactct cgc
//
