LOCUS       sequence-375510-        4144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL187
FEATURES             Location/Qualifiers
     source          1..4144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(902..1001)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1113..1132)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1191..1210)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1784..2317)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2506..2511,2512..2528,2529..2534))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3191..3779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3680..3699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3914..3941)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4033..4119)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgtat cttcctgaat tggtcaacat ttttaatggc
      181 atgcatgttg gaccttttat agaagaaatg atattttatt attgcccaag actccaaagt
      241 ctttccaaat ctgagttgtc aagcaaatct ttgaagatca tcaaaggtga aagcatctgg
      301 tgggaagcat tgaaatggaa tgaagcagag tggggagatg ctggtcgacc caattatttt
      361 gagcgaattt tttcaccaat taatgaggag gctgacatga tgaaccaact agctgcactt
      421 caagagactc aactaaatga atatcacaat acgacatatc agggaatttc aagttctaaa
      481 cagtcgatag caaaaatatt agccccagtt tcatctgtgg gtaggggtag ttcaaacagt
      541 gaagcccaaa agcggaaagt cgttgctgag ccaaagatat ttcaagaggt aacaaaaaag
      601 ggttataaac gaagagaagt ttggacagca aagatacgtg tcaatgcgga agggtgttat
      661 gatgatggat atatctggag aaaatatggg aaaaggcaaa ttcttggagc taagtatcca
      721 agagaatatt acagatgcgc acataaattt gtacattggt gcccagcaag aaaacgagtt
      781 caaaggttag acgaaaaccc agcaacaatt gagataatct acgagcacag acatacatgc
      841 gccagagaac agaatttgat cattaagagt ggggatgagg caagtcaatc ctgcaagtta
      901 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
      961 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg
     1021 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     1081 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     1141 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1201 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     1261 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1321 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     1381 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     1441 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     1501 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     1561 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     1621 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     1681 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     1741 ggggaaatta ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     1801 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     1861 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     1921 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     1981 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     2041 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     2101 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     2161 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     2221 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     2281 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     2341 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     2401 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     2461 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     2521 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     2581 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
     2641 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
     2701 tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg
     2761 gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt
     2821 ttgttcgccc aggactctag ctatagttct agtggttggc tactaatagg ttgtattgat
     2881 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2941 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3001 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3061 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc caggaagagt
     3901 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     3961 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4021 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4081 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4141 tcgc
//
