LOCUS       sequence-375511-        4402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL189
FEATURES             Location/Qualifiers
     source          1..4402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1160..1259)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1371..1390)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1449..1468)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2042..2575)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2764..2769,2770..2786,2787..2792))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3449..4037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3938..3957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4172..4199)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4291..4377)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatggct atgcccaacc tccaaaggtt aaagatggaa
      181 ttcaatggta gcacagtgga gcaatacaac cttgaaaatg tgggcttcaa gcacttgtca
      241 ggtgtcaagg tcagcgcaaa aatttgcatt gccggtacta gtgaagctaa cagaatggct
      301 atagcctctg tactgaacaa tgccatcagg caaaatacaa gaatatcaag tgtaatcata
      361 cggttcatca acagggttat aaaagatctt ccgattgtcc ccacccctca cacggatcat
      421 atgcatgaaa atctgaggct ggataagagt gtcaggtcct ggcctaccca gtgcgatggc
      481 tgcaaggagc ttggtgccgg ccacaggttc aagtgcgagc aatgcaactc caaggtgtac
      541 tatgatatgt gctgcgcaac agcgcctcac accctgaaac accctctgtt cccaggctcc
      601 gtttttcggt tcctccgaaa gcctctggcc tctgaatgcg gtagggcctg tgatgcatgt
      661 ggtgacctca tgcacggctt cgtctaccac tgctttgagc gtggcctcga cctccaccca
      721 cgctgtgcga ggctgcctgt tcgcactgca aacgtaaagg gctatgtcat ggagcttcgc
      781 agggtgtcgg cttgcagcag atgttgcatt tgcatgtgtg gcaaggaggg ttatcgcaac
      841 aagttttggt cctaccgctc cagtcaggaa ggccaggaca ttaacgtgca tatggcgtgt
      901 ttaaaagact tggcatcaaa atcacatgaa accctaacta aactgtatga gatcctaatg
      961 ggtgaatcac agtggacgcc aacagaggtt ttaggcgaag atacatccga ccaattagtt
     1021 gtcagcagtg cggaggatgg tgatcgtacg ccactgagac aagatgaaat tacagaggag
     1081 tttcagcaca aggatgaagt atgtaatgtt ctagagcatg gacgggagga actccggaag
     1141 aaagaaaccg ataccaggga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     1201 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1261 catccagctg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1321 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1381 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1441 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1501 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1561 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1621 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1681 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1741 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1801 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1861 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1921 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1981 taaccttatt tttgacgagg ggaaattagg ccgggaagcc gatctcggct tgaacgaatt
     2041 gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact
     2101 ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag
     2161 caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc
     2221 tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa
     2281 acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa
     2341 gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt
     2401 gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg
     2461 atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta
     2521 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg
     2581 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     2641 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2701 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     2761 tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac
     2821 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     2881 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     2941 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     3001 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     3061 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
     3121 ctaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3181 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3241 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3301 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3361 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4081 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4141 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     4201 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     4261 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     4321 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     4381 cctggcagtt ccctactctc gc
//
